GRM2 抗体 (N-Term)
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- 抗原 See all GRM2 抗体
- GRM2 (Glutamate Receptor, Metabotropic 2 (GRM2))
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抗原表位
- N-Term
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适用
- 人, 大鼠, 小鼠, Cow, 犬, 兔, Hamster, Bat, Pig
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This GRM2 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)
- 品牌
- IHC-plus™
- 特异性
- Human GRM2 / MGLUR2. BLAST analysis of the peptide immunogen showed no homology with other human proteins, except GRM3 (42 % ).
- 预测反应
- Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Bovine, Dog, Bat, Rabbit, Pig (100%) Chimpanzee, Monkey, Horse, Platypus (95%).
- 纯化方法
- Immunoaffinity purified
- 免疫原
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Synthetic 19 amino acid peptide from N-terminal extracellular domain of human GRM2 / MGLUR2. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Bovine, Dog, Bat, Rabbit, Pig (100%), Chimpanzee, Monkey, Horse, Platypus (95%).
Type of Immunogen: Synthetic peptide - Top Product
- Discover our top product GRM2 Primary Antibody
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anti-Glutamate Receptor, Metabotropic 2 (GRM2) (AA 414-558) antibody
GRM2 适用: 人 ELISA, WB, IHC, ICC, FACS, Neut 宿主: 小鼠 Monoclonal 4A10B9 unconjugated
anti-Glutamate Receptor, Metabotropic 2 (GRM2) (N-Term) antibodyGRM2 适用: 人, 兔, 猴 IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Glutamate Receptor, Metabotropic 2 (GRM2) (AA 109-121), (Extracellular), (N-Term) antibodyGRM2 适用: 人, 大鼠, 小鼠 WB, IHC, IF, ICC, LCI 宿主: 兔 Polyclonal unconjugated
anti-Glutamate Receptor, Metabotropic 2 (GRM2) (AA 93-221) antibodyGRM2 适用: 人 ELISA, WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Glutamate Receptor, Metabotropic 2 (GRM2) (N-Term) antibodyGRM2 适用: 人, 大鼠, 犬, 兔, Hamster, Bat IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Glutamate Receptor, Metabotropic 2 (GRM2) (Internal Region) antibodyVerified GRM2 适用: 人 ELISA, WB, IHC 宿主: 山羊 Polyclonal unconjugated
anti-Glutamate Receptor, Metabotropic 2 (GRM2) (AA 801-872) antibodyGRM2 适用: 大鼠 ELISA, WB, IF (p), IHC (p), IF (cc), ICC, IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Glutamate Receptor, Metabotropic 2 (GRM2) (AA 681-715) antibodyGRM2 适用: 人 WB 宿主: 兔 Polyclonal RB57773 unconjugated
anti-Glutamate Receptor, Metabotropic 2 (GRM2) antibodyGRM2 适用: 人, 大鼠, 小鼠 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Glutamate Receptor, Metabotropic 2 (GRM2) (AA 414-506) antibodyGRM2 适用: 人 ELISA, WB 宿主: 小鼠 Polyclonal unconjugated
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- 应用备注
- Approved: ICC, IHC, IHC-P (5 - 10 μg/mL), WB
- 说明
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Target Species of Antibody: Human
- 实验流程
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The IHC-pro Immunohistochemistry Protocol
Tissue Preparation
Formalin fixation and embedding in paraffin wax
Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.
Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.
Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.
Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.
Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip - 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- Lot specific
- 缓冲液
- PBS, less than 0.1 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
- 有效期
- 12 months
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- 抗原
- GRM2 (Glutamate Receptor, Metabotropic 2 (GRM2))
- 别名
- GRM2 / MGLUR2 (GRM2 产品)
- 别名
- GLUR2 antibody, GPRC1B antibody, MGLUR2 antibody, mGlu2 antibody, 4930441L02Rik antibody, Gprc1b antibody, mGluR2 antibody, mGluR7 antibody, glutamate metabotropic receptor 2 antibody, glutamate receptor, metabotropic 2 antibody, GRM2 antibody, Grm2 antibody
- 背景
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Name/Gene ID: GRM2
Subfamily: Metabotropic glutamate
Family: GPCR
Synonyms: GRM2, Glutamate receptor homolog, GPRC1B, MGlu2, Metabotropic, GLUR2, MGLUR2 - 基因ID
- 2912
- 途径
- cAMP Metabolic Process, Dicarboxylic Acid Transport
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