ADGRV1 / GPR98 抗体 (N-Term)
-
- 抗原 See all ADGRV1 / GPR98 (ADGRV1) products
- ADGRV1 / GPR98 (ADGRV1) (G-Protein Coupled Receptor 98 (ADGRV1))
- 抗原表位
- N-Term
-
适用
- 人
-
宿主
- 兔
-
克隆类型
- 多克隆
-
标记
- This ADGRV1 / GPR98 antibody is un-conjugated
-
应用范围
- Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 品牌
- IHC-plus™
- 特异性
- Human GPR98. BLAST analysis of the peptide immunogen showed no homology with other human proteins, except LAMC2 (53 % ).
- 预测反应
- Percent identity with other species by BLAST analysis: Human (100%) Gorilla, Gibbon, Monkey, Marmoset (95%) Elephant, Horse (89%) Dog, Panda (84%).
- 纯化方法
- Immunoaffinity purified
- 免疫原
-
Synthetic 19 amino acid peptide from N-terminal extracellular domain of human GPR98. Percent identity with other species by BLAST analysis: Human (100%), Gorilla, Gibbon, Monkey, Marmoset (95%), Elephant, Horse (89%), Dog, Panda (84%).
Type of Immunogen: Synthetic peptide
-
anti-G-Protein Coupled Receptor 98 (ADGRV1) (N-Term) antibody
ADGRV1 适用: 人, 马 IHC, IHC (p), WB 宿主: 兔 Polyclonal unconjugated
-
- 应用备注
-
Approved: IHC, IHC-P (15 μg/mL)
Usage: Proteinase K treatment is required for antigen retrieval. - 说明
-
Target Species of Antibody: Human
- 实验流程
-
The IHC-pro Immunohistochemistry Protocol
Tissue Preparation
Formalin fixation and embedding in paraffin wax
Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.
Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.
Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.
Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.
Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip - 限制
- 仅限研究用
-
- 状态
- Liquid
- 浓度
- Lot specific
- 缓冲液
- PBS, less than 0.1 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
- 有效期
- 12 months
-
- 抗原
- ADGRV1 / GPR98 (ADGRV1) (G-Protein Coupled Receptor 98 (ADGRV1))
- 别名
- ADGRV1 / GPR98 (ADGRV1 产品)
- 背景
-
Name/Gene ID: ADGRV1
Subfamily: Orphan-A
Family: GPCR
Synonyms: ADGRV1, FEB4, KIAA0686, G-protein coupled receptor 98, MASS1, VLGR1b, Usher syndrome type-2C protein, USH2B, USH2C, G protein-coupled receptor 98, GPR98, KIAA1943, VLGR1 - 基因ID
- 84059
-
