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GPR4 抗体 (C-Term)

GPR4 适用: 人, 犬, 猴 IHC, IHC (p), ICC 宿主: 兔 Polyclonal unconjugated
产品编号 ABIN1048818
发货至: 中国
  • 抗原 See all GPR4 抗体
    GPR4 (G Protein-Coupled Receptor 4 (GPR4))
    抗原表位
    • 5
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    C-Term
    适用
    • 31
    • 14
    • 13
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    人, 犬, 猴
    宿主
    • 31
    克隆类型
    • 31
    多克隆
    标记
    • 20
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This GPR4 antibody is un-conjugated
    应用范围
    • 10
    • 8
    • 6
    • 5
    • 4
    • 2
    • 1
    • 1
    Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)
    品牌
    IHC-plus™
    特异性
    Human GPR4. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
    预测反应
    Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Dog, Panda (100%) Mouse, Rat, Bovine, Bat, Pig (95%) Rabbit (89%).
    纯化方法
    Immunoaffinity purified
    免疫原
    Synthetic 19 amino acid peptide from C-terminus of human GPR4. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Dog, Panda (100%), Mouse, Rat, Bovine, Bat, Pig (95%), Rabbit (89%).

    Type of Immunogen: Synthetic peptide
  • 应用备注
    Approved: ICC, IHC, IHC-P (24 μg/mL)
    说明

    Target Species of Antibody: Human

    实验流程

    The IHC-pro Immunohistochemistry Protocol

    Tissue Preparation
    Formalin fixation and embedding in paraffin wax

    Tissue Sectioning
    Make 4-µm sections and place on pre-cleaned and charged microscope slides.
    Heat in a tissue-drying oven for 45 minutes at 60°C
    Deparaffinization
    Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

    Rehydration
    Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
    Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
    Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
    Rinse slides in gentle running distilled water – 5 minutes at room temperature.

    Antigen retrieval
    Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
    Remove from heat and let stand at room temperature in buffer - 20 minutes
    Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

    Immunostaining
    Do not allow tissues to dry at any time during the staining procedure.
    Apply a universal protein block – 20 minutes at room temperature.
    Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
    Rinse slides in 1X TBST - 1 minute at room temperature.
    Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
    Rinse slides 1X TBST – 1 minute at room temperature.
    Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
    Rinse slides in 1X TBST - 1 minute at room temperature.
    Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
    Wash slides in distilled water – 1 minute at room temperature.

    Dehydrate
    This method should only be used if the chromogen substrate is alcohol insoluble.
    Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
    Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
    Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
    Wash slides in 3 changes of xylene – 1 minute each at room temperature.
    Apply coverslip

    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    Lot specific
    缓冲液
    PBS, less than 0.1 % sodium azide.
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    4 °C,-20 °C
    储存方法
    Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
    有效期
    12 months
  • 抗原
    GPR4 (G Protein-Coupled Receptor 4 (GPR4))
    别名
    GPR4 (GPR4 产品)
    别名
    G protein-coupled receptor 4 antibody, GPR4 antibody, Gpr4 antibody
    背景
    Name/Gene ID: GPR4
    Subfamily: Lysophospholipid/Lysosphingolipid
    Family: GPCR

    Synonyms: GPR4, G-protein coupled receptor 19, G-protein coupled receptor 4, Gpr6c, G protein-coupled receptor 4
    基因ID
    2828
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