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- 抗原 See all GPR150 抗体
- GPR150 (G Protein-Coupled Receptor 150 (GPR150))
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抗原表位
- C-Term
- 适用
- 人, 猴
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This GPR150 antibody is un-conjugated
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应用范围
- Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC)
- 品牌
- IHC-plus™
- 特异性
- Human GPR150. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
- 预测反应
- Percent identity with other species by BLAST analysis: Human, Gorilla, Monkey (100%) Mouse, Rat, Hamster, Panda, Bovine, Bat, Pig (89%).
- 纯化方法
- Immunoaffinity purified
- 免疫原
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Synthetic 18 amino acid peptide from C-Terminus of human GPR150. Percent identity with other species by BLAST analysis: Human, Gorilla, Monkey (100%), Mouse, Rat, Hamster, Panda, Bovine, Bat, Pig (89%).
Type of Immunogen: Synthetic peptide
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anti-G Protein-Coupled Receptor 150 (GPR150) (N-Term) antibody
GPR150 适用: 人 IHC (p), IHC 宿主: 兔 Polyclonal unconjugated
anti-G Protein-Coupled Receptor 150 (GPR150) (N-Term) antibodyGPR150 适用: 人 WB, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-G Protein-Coupled Receptor 150 (GPR150) (AA 345-394) antibodyGPR150 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-G Protein-Coupled Receptor 150 (GPR150) (AA 361-410) antibodyGPR150 适用: 人 WB, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-G Protein-Coupled Receptor 150 (GPR150) (AA 366-395), (C-Term) antibodyGPR150 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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Approved: IHC, IHC-P (5 μg/mL)
Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after proteinase K antigen retrieval. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. - 说明
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Target Species of Antibody: Human
- 实验流程
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The IHC-pro Immunohistochemistry Protocol
Tissue Preparation
Formalin fixation and embedding in paraffin wax
Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.
Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.
Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.
Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.
Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip - 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- Lot specific
- 缓冲液
- PBS, less than 0.1 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
- 有效期
- 12 months
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- 抗原
- GPR150 (G Protein-Coupled Receptor 150 (GPR150))
- 别名
- GPR150 (GPR150 产品)
- 别名
- RGD1564729 antibody, PGR11 antibody, C030001A19Rik antibody, G protein-coupled receptor 150 antibody, GPR150 antibody, Gpr150 antibody
- 背景
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Name/Gene ID: GPR150
Subfamily: Orphan-A
Family: GPCR
Synonyms: GPR150, G protein-coupled receptor 150, PGR11, GPRv40 - 基因ID
- 285601
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