GLP1R 抗体 (N-Term)
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- 抗原 See all GLP1R 抗体
- GLP1R (Glucagon-Like Peptide 1 Receptor (GLP1R))
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抗原表位
- N-Term
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适用
- 人, 小鼠
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This GLP1R antibody is un-conjugated
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应用范围
- Immunohistochemistry (IHC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))
- 品牌
- IHC-plus™
- 特异性
- Human GLP1R. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
- 纯化方法
- Immunoaffinity purified
- 免疫原
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Synthetic 16 amino acid peptide from N-terminal extracellular domain of human GLP1R. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey (100%), Marmoset, Mouse, Rat, Sheep, Bovine, Hamster, Elephant, Rabbit, Pig (94%).
Type of Immunogen: Synthetic peptide - Top Product
- Discover our top product GLP1R Primary Antibody
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anti-Glucagon-Like Peptide 1 Receptor (GLP1R) (AA 101-200) antibody
GLP1R 适用: 人, 小鼠, 大鼠 WB, ELISA, FACS, IP, ICC, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Glucagon-Like Peptide 1 Receptor (GLP1R) (AA 25-145) antibodyGLP1R 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Glucagon-Like Peptide 1 Receptor (GLP1R) (Internal Region) antibodyVerified GLP1R 适用: 人, 大鼠, Pig WB, ELISA, IF, FACS 宿主: 山羊 Polyclonal unconjugated
anti-Glucagon-Like Peptide 1 Receptor (GLP1R) (AA 24-145) antibodyGLP1R 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Glucagon-Like Peptide 1 Receptor (GLP1R) (AA 22-116) antibodyGLP1R 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Glucagon-Like Peptide 1 Receptor (GLP1R) (2nd Extracellular Loop), (AA 288-301) antibodyGLP1R 适用: 人, 小鼠, 大鼠 WB, IHC, IF, ICC, LCI 宿主: 兔 Polyclonal unconjugated
anti-Glucagon-Like Peptide 1 Receptor (GLP1R) (Cytoplasmic Domain) antibodyGLP1R 适用: 人, 小鼠, 犬, 兔, 猴, Pig, Bat, Hamster, 马 IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Glucagon-Like Peptide 1 Receptor (GLP1R) (Center) antibodyGLP1R 适用: 人 WB, IHC, IF, IC 宿主: 兔 Polyclonal unconjugated
anti-Glucagon-Like Peptide 1 Receptor (GLP1R) (AA 101-200) antibody (PE)GLP1R 适用: 人, 小鼠, 大鼠 WB, FACS 宿主: 兔 Polyclonal PE
anti-Glucagon-Like Peptide 1 Receptor (GLP1R) (AA 24-139) antibodyGLP1R 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
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- 应用备注
- Approved: IF, IHC, IHC-Fr, IHC-P (3 - 4 μg/mL)
- 说明
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Target Species of Antibody: Human
- 实验流程
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The IHC-pro Immunohistochemistry Protocol
Tissue Preparation
Formalin fixation and embedding in paraffin wax
Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.
Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.
Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.
Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.
Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip - 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- Lot specific
- 缓冲液
- PBS, less than 0.1 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
- 有效期
- 12 months
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- 抗原
- GLP1R (Glucagon-Like Peptide 1 Receptor (GLP1R))
- 别名
- GLP1R/GLP-1R/GLP-1 Receptor (GLP1R 产品)
- 别名
- GLP1R antibody, LOC100228311 antibody, GLP-1R antibody, GLP1Rc antibody, Glip antibody, RATGL1RCP antibody, glucagon like peptide 1 receptor antibody, glucagon-like peptide 1 receptor antibody, GLP1R antibody, Glp1r antibody
- 背景
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Name/Gene ID: GLP1R
Subfamily: Glucagon
Family: GPCR
Synonyms: GLP1R, GLP-1-R, GLP-1 receptor, GLP-1R, GLP-I receptor, Glp1 receptor - 基因ID
- 2740
- 途径
- Positive Regulation of Peptide Hormone Secretion, Hormone Transport, cAMP Metabolic Process, Feeding Behaviour
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