-
- 抗原 See all GALR3 抗体
- GALR3 (Galanin Receptor 3 (GALR3))
-
抗原表位
- C-Term
-
适用
- 人, 大鼠, 小鼠, 犬
-
宿主
- 兔
-
克隆类型
- 多克隆
-
标记
- This GALR3 antibody is un-conjugated
-
应用范围
- Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 品牌
- IHC-plus™
- 特异性
- Human GALR3. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
- 预测反应
- Percent identity with other species by BLAST analysis: Human, Gorilla, Marmoset, Mouse, Rat, Dog, Elephant (100%) Monkey, Rabbit, Pig (93%).
- 纯化方法
- Immunoaffinity purified
- 免疫原
-
Synthetic 15 amino acid peptide from C-terminus of human GALR3. Percent identity with other species by BLAST analysis: Human, Gorilla, Marmoset, Mouse, Rat, Dog, Elephant (100%), Monkey, Rabbit, Pig (93%).
Type of Immunogen: Synthetic peptide - Top Product
- Discover our top product GALR3 Primary Antibody
-
anti-Galanin Receptor 3 (GALR3) (Cytoplasmic Domain) antibody
GALR3 适用: 人, 大鼠 IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Galanin Receptor 3 (GALR3) (Extracellular Domain) antibodyGALR3 适用: 人, 大鼠, 小鼠, 猴, Pig, Cow IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Galanin Receptor 3 (GALR3) (C-Term) antibodyGALR3 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Galanin Receptor 3 (GALR3) (C-Term) antibodyVerified GALR3 适用: 人 WB, ELISA, IHC 宿主: 山羊 Polyclonal unconjugated
anti-Galanin Receptor 3 (GALR3) (2nd Extracellular Loop), (AA 159-172) antibodyGALR3 适用: 人, 大鼠, 小鼠 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
-
- 应用备注
- Approved: IHC, IHC-P (11 - 22 μg/mL)
- 说明
-
Target Species of Antibody: Human
- 实验流程
-
The IHC-pro Immunohistochemistry Protocol
Tissue Preparation
Formalin fixation and embedding in paraffin wax
Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.
Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.
Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.
Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.
Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip - 限制
- 仅限研究用
-
- 状态
- Liquid
- 浓度
- Lot specific
- 缓冲液
- PBS, less than 0.1 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
- 有效期
- 12 months
-
- 抗原
- GALR3 (Galanin Receptor 3 (GALR3))
- 别名
- GALR3 / Galanin Receptor 3 (GALR3 产品)
- 别名
- GALR3 antibody, Galnr3 antibody, galanin receptor 3 antibody, GALR3 antibody, LOC100037699 antibody, Galr3 antibody
- 背景
-
Name/Gene ID: GALR3
Subfamily: Galanin
Family: GPCR
Synonyms: GALR3, Gal receptor-3, Galanin r3 receptor, Gal-r3, Galanin receptor 3, Galanin receptor galr3, Galanin receptor subtype 3, Gal3 receptor, GAL3-R, Galanin 2 receptor homolog, Galanin receptor type 3, GALNR3, GALR-3 - 基因ID
- 8484
- 途径
- cAMP Metabolic Process, Feeding Behaviour
-
You are here:
