GABRA3 抗体 (N-Term)
-
- 抗原 See all GABRA3 抗体
- GABRA3 (gamma-aminobutyric Acid (GABA) A Receptor, alpha 3 (GABRA3))
-
抗原表位
- N-Term
-
适用
- 人, 大鼠, 小鼠, 犬, Cow, 兔, 马, 猴, Bat
-
宿主
- 兔
-
克隆类型
- 多克隆
-
标记
- This GABRA3 antibody is un-conjugated
-
应用范围
- Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 品牌
- IHC-plus™
- 特异性
- Human GABRA3. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
- 预测反应
- Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Elephant, Dog, Bat, Bovine, Horse, Rabbit (100%) Opossum, Platypus (87%).
- 纯化方法
- Immunoaffinity purified
- 免疫原
-
Synthetic 15 amino acid peptide from N-Terminus of human GABRA3. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Elephant, Dog, Bat, Bovine, Horse, Rabbit (100%), Opossum, Platypus (87%).
Type of Immunogen: Synthetic peptide - Top Product
- Discover our top product GABRA3 Primary Antibody
-
anti-gamma-aminobutyric Acid (GABA) A Receptor, alpha 3 (GABRA3) antibody
GABRA3 适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-gamma-aminobutyric Acid (GABA) A Receptor, alpha 3 (GABRA3) antibodyGABRA3 适用: 人, 大鼠, 小鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-gamma-aminobutyric Acid (GABA) A Receptor, alpha 3 (GABRA3) (N-Term) antibodyGABRA3 适用: 大鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-gamma-aminobutyric Acid (GABA) A Receptor, alpha 3 (GABRA3) (AA 29-43) antibodyGABRA3 适用: 大鼠, 小鼠 WB, IHC, ICC 宿主: 兔 Polyclonal unconjugated
anti-gamma-aminobutyric Acid (GABA) A Receptor, alpha 3 (GABRA3) (AA 480-492) antibodyGABRA3 适用: 人, 大鼠, 小鼠, Cow, 兔, 马, 猴, Bat, Hamster IHC, IHC (p) 宿主: 山羊 Polyclonal unconjugated
anti-gamma-aminobutyric Acid (GABA) A Receptor, alpha 3 (GABRA3) (C-Term) antibodyGABRA3 适用: 人, 兔, 猴 IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-gamma-aminobutyric Acid (GABA) A Receptor, alpha 3 (GABRA3) (Middle Region) antibodyGABRA3 适用: 人, 大鼠, 小鼠, 犬, Cow, 兔, 马, 豚鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-gamma-aminobutyric Acid (GABA) A Receptor, alpha 3 (GABRA3) (AA 29-43), (Extracellular), (N-Term) antibodyGABRA3 适用: 人, 大鼠, 小鼠 WB, IHC, ICC, IF, IP, LCI 宿主: 兔 Polyclonal unconjugated
anti-gamma-aminobutyric Acid (GABA) A Receptor, alpha 3 (GABRA3) antibodyGABRA3 适用: 大鼠 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-gamma-aminobutyric Acid (GABA) A Receptor, alpha 3 (GABRA3) (AA 28-61), (N-Term) antibodyGABRA3 适用: 人 WB 宿主: 兔 Polyclonal RB50699 unconjugated
-
- 应用备注
- Approved: IHC, IHC-P (5 μg/mL)
- 说明
-
Target Species of Antibody: Human
- 实验流程
-
The IHC-pro Immunohistochemistry Protocol
Tissue Preparation
Formalin fixation and embedding in paraffin wax
Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.
Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.
Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.
Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.
Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip - 限制
- 仅限研究用
-
- 状态
- Liquid
- 浓度
- Lot specific
- 缓冲液
- PBS, less than 0.1 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
- 有效期
- 12 months
-
- 抗原
- GABRA3 (gamma-aminobutyric Acid (GABA) A Receptor, alpha 3 (GABRA3))
- 别名
- GABRA3 (GABRA3 产品)
- 别名
- GABRA3 antibody, Gabra-3 antibody, si:ch211-113k9.1 antibody, gamma-aminobutyric acid type A receptor alpha3 subunit antibody, gamma-aminobutyric acid (GABA) A receptor, alpha 3 antibody, gamma-aminobutyric acid (GABA) A receptor, subunit alpha 3 antibody, gamma-aminobutyric acid receptor subunit alpha-3 antibody, GABRA3 antibody, gabra3 antibody, Gabra3 antibody, LOC100653496 antibody
- 背景
-
Name/Gene ID: GABRA3
Subfamily: GABA A receptor
Family: Ion Channel
Synonyms: GABRA3, GABA(A) receptor, alpha 3, GABA A receptor alpha 3 - 基因ID
- 2556
-
