FZD9 抗体 (N-Term)
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- 抗原 See all FZD9 抗体
- FZD9 (Frizzled Family Receptor 9 (FZD9))
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抗原表位
- N-Term
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适用
- 人, 犬, Cow
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This FZD9 antibody is un-conjugated
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应用范围
- Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)
- 品牌
- IHC-plus™
- 特异性
- Human FZD9 / Frizzled 9. BLAST analysis of the peptide immunogen showed no homology with other human proteins, except FZD10 (67 % ).
- 预测反应
- Percent identity with other species by BLAST analysis: Human, Gibbon, Dog, Bovine (100%) Mouse, Rat, Hamster (93%) Rabbit, Opossum, Turkey, Chicken, Platypus, Zebrafish (87%) Stickleback, Pufferfish (80%).
- 纯化方法
- Immunoaffinity purified
- 免疫原
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Synthetic 15 amino acid peptide from N-terminal extracellular domain of human FZD9 / Frizzled 9. Percent identity with other species by BLAST analysis: Human, Gibbon, Dog, Bovine (100%), Mouse, Rat, Hamster (93%), Rabbit, Opossum, Turkey, Chicken, Platypus, Zebrafish (87%), Stickleback, Pufferfish (80%).
Type of Immunogen: Synthetic peptide - Top Product
- Discover our top product FZD9 Primary Antibody
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anti-Frizzled Family Receptor 9 (FZD9) (AA 1-50) antibody
FZD9 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Frizzled Family Receptor 9 (FZD9) (AA 185-270) antibodyFZD9 适用: 人 WB, ELISA, ICC, IF (cc) 宿主: 兔 Polyclonal unconjugated
anti-Frizzled Family Receptor 9 (FZD9) (AA 23-229) antibodyFZD9 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Frizzled Family Receptor 9 (FZD9) (C-Term) antibodyFZD9 适用: 人, 小鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Frizzled Family Receptor 9 (FZD9) (C-Term) antibodyFZD9 适用: 人, 小鼠, 大鼠 WB, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Frizzled Family Receptor 9 (FZD9) (AA 181-230) antibodyFZD9 适用: 人, 犬, Cow, 豚鼠, 斑马鱼 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Frizzled Family Receptor 9 (FZD9) (N-Term) antibodyFZD9 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Frizzled Family Receptor 9 (FZD9) antibodyFZD9 适用: 人, 犬 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Frizzled Family Receptor 9 (FZD9) (N-Term) antibodyFZD9 适用: 人, 小鼠, 大鼠, 犬, Cow, 兔, 豚鼠, 斑马鱼 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Frizzled Family Receptor 9 (FZD9) (C-Term) antibodyFZD9 适用: 人 WB, ELISA, IF 宿主: 兔 Polyclonal unconjugated
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- 应用备注
- Approved: ICC, IHC, IHC-P (13 μg/mL)
- 说明
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Target Species of Antibody: Human
- 实验流程
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The IHC-pro Immunohistochemistry Protocol
Tissue Preparation
Formalin fixation and embedding in paraffin wax
Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.
Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.
Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.
Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.
Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip - 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- Lot specific
- 缓冲液
- PBS, less than 0.1 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
- 有效期
- 12 months
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- 抗原
- FZD9 (Frizzled Family Receptor 9 (FZD9))
- 别名
- FZD9 / Frizzled 9 (FZD9 产品)
- 别名
- CD349 antibody, FZD3 antibody, mfz9 antibody, Fz-9 antibody, cFz-9 antibody, fz11 antibody, fzd9 antibody, fzx antibody, hm:zehl0603 antibody, zehl0603 antibody, zg11 antibody, frizzled class receptor 9 antibody, frizzled class receptor 9a antibody, frizzled class receptor 9b antibody, FZD9 antibody, Fzd9 antibody, fzd9a antibody, fzd9b antibody
- 背景
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Name/Gene ID: FZD9
Subfamily: Frizzled
Family: GPCR
Synonyms: FZD9, CD349, Frizzled homolog 9, Fz-9, Frizzled family receptor 9, Frizzled-9, Frizzled 9, Fz9, HFz9, CD349 antigen, Frizzled homolog fzd3, FzE6 - 基因ID
- 8326
- 途径
- WNT signaling
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