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- 抗原 See all FZD10 抗体
- FZD10 (Frizzled Family Receptor 10 (FZD10))
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抗原表位
- N-Term
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适用
- 人, 猴
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This FZD10 antibody is un-conjugated
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应用范围
- Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 品牌
- IHC-plus™
- 特异性
- Human FZD10 / Frizzled 10. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
- 预测反应
- Percent identity with other species by BLAST analysis: Human, Monkey (100%) Gibbon, Hamster, Panda, Dog (93%) Marmoset, Mouse, Bovine, Horse, Pig (86%).
- 纯化方法
- Immunoaffinity purified
- 免疫原
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Synthetic 14 amino acid peptide from N-terminal extracellular domain of human FZD10 / Frizzled 10. Percent identity with other species by BLAST analysis: Human, Monkey (100%), Gibbon, Hamster, Panda, Dog (93%), Marmoset, Mouse, Bovine, Horse, Pig (86%).
Type of Immunogen: Synthetic peptide - Top Product
- Discover our top product FZD10 Primary Antibody
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anti-Frizzled Family Receptor 10 (FZD10) (AA 101-200) antibody
FZD10 适用: 人, 小鼠, 大鼠 WB, ELISA, IF (cc), IF (p), IHC (p), IHC (fro), ICC 宿主: 兔 Polyclonal unconjugated
anti-Frizzled Family Receptor 10 (FZD10) (N-Term) antibodyFZD10 适用: 人, 小鼠 WB, ELISA, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Frizzled Family Receptor 10 (FZD10) antibodyFZD10 适用: 人, 小鼠, 猴 WB, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Frizzled Family Receptor 10 (FZD10) (N-Term) antibodyFZD10 适用: 人, 小鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-Frizzled Family Receptor 10 (FZD10) antibodyFZD10 适用: 人, 小鼠, Pig, Cow, 犬, 马 IF 宿主: 兔 Polyclonal unconjugated
anti-Frizzled Family Receptor 10 (FZD10) (AA 177-212), (N-Term) antibodyFZD10 适用: 小鼠 WB 宿主: 兔 Polyclonal RB52084 unconjugated
anti-Frizzled Family Receptor 10 (FZD10) (Internal Region) antibodyFZD10 适用: 人, 小鼠, 猴 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Frizzled Family Receptor 10 (FZD10) (AA 36-85) antibodyFZD10 适用: 人, 小鼠, Pig, 猴, Cow, 犬, 马, 小鸡, 斑马鱼 WB 宿主: 兔 Polyclonal unconjugated
anti-Frizzled Family Receptor 10 (FZD10) antibodyFZD10 适用: 人 FACS 宿主: 兔 Chimeric unconjugated Fc fragment
anti-Frizzled Family Receptor 10 (FZD10) (AA 135-230) antibodyFZD10 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
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- 应用备注
- Approved: IHC, IHC-P (6 - 16 μg/mL)
- 说明
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Target Species of Antibody: Human
- 实验流程
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The IHC-pro Immunohistochemistry Protocol
Tissue Preparation
Formalin fixation and embedding in paraffin wax
Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.
Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.
Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.
Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.
Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip - 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- Lot specific
- 缓冲液
- PBS, less than 0.1 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
- 有效期
- 12 months
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- 抗原
- FZD10 (Frizzled Family Receptor 10 (FZD10))
- 别名
- FZD10 / Frizzled 10 (FZD10 产品)
- 别名
- CD350 antibody, FZ-10 antibody, Fz10 antibody, FzE7 antibody, hFz10 antibody, cFz-10 antibody, Fz-10 antibody, Xfr9 antibody, Xfz10 antibody, frizzled-10 antibody, frizzled10 antibody, fz10 antibody, fze7 antibody, hfz10 antibody, fk48e04 antibody, fz4 antibody, fzb antibody, wu:fk48e04 antibody, zg04 antibody, Xfz10A antibody, fzd10a antibody, Xfz10B antibody, Xfz9 antibody, fzd10b antibody, fzd9 antibody, frizzled class receptor 10 antibody, frizzled class receptor 10 L homeolog antibody, frizzled class receptor 10 S homeolog antibody, FZD10 antibody, fzd10 antibody, Fzd10 antibody, fzd10.L antibody, fzd10.S antibody
- 背景
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Name/Gene ID: FZD10
Subfamily: Frizzled
Family: GPCR
Synonyms: FZD10, CD350 antigen, CD350, FZ-10, Frizzled homolog 10, HFz10, Frizzled 10, Frizzled family receptor 10, Frizzled-10, FzE7, Fz10 - 基因ID
- 11211
- 途径
- WNT signaling
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