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- 抗原 See all FSHR 抗体
- FSHR (Follicle Stimulating Hormone Receptor (FSHR))
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抗原表位
- N-Term
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适用
- 人, 猴
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This FSHR antibody is un-conjugated
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应用范围
- Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 品牌
- IHC-plus™
- 特异性
- Human FSHR. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
- 预测反应
- Percent identity with other species by BLAST analysis: Human, Gorilla, Monkey, Marmoset (100%) Gibbon, Dog, Bat, Elephant, Rabbit, Horse, Pig, Guinea pig (94%) Bovine, Cat, Goat (88%) Mouse, Rat, Sheep (82%).
- 纯化方法
- Immunoaffinity purified
- 免疫原
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Synthetic 17 amino acid peptide from N-terminal extracellular domain of human FSHR. Percent identity with other species by BLAST analysis: Human, Gorilla, Monkey, Marmoset (100%), Gibbon, Dog, Bat, Elephant, Rabbit, Horse, Pig, Guinea pig (94%), Bovine, Cat, Goat (88%), Mouse, Rat, Sheep (82%).
Type of Immunogen: Synthetic peptide - Top Product
- Discover our top product FSHR Primary Antibody
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anti-Follicle Stimulating Hormone Receptor (FSHR) (AA 201-300) antibody
FSHR 适用: 人, 小鼠, 大鼠 WB, ELISA, FACS, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Follicle Stimulating Hormone Receptor (FSHR) (AA 631-695) antibodyFSHR 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Follicle Stimulating Hormone Receptor (FSHR) (AA 18-366) antibodyFSHR 适用: 人 ELISA, FACS 宿主: 小鼠 Monoclonal 3D5G9 unconjugated
anti-Follicle Stimulating Hormone Receptor (FSHR) (AA 21-100) antibodyFSHR 适用: 人, 小鼠, 大鼠 WB, FACS, IF (cc), IF (p), IHC (p), ICC, IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Follicle Stimulating Hormone Receptor (FSHR) (AA 18-120) antibodyFSHR 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
anti-Follicle Stimulating Hormone Receptor (FSHR) (N-Term) antibodyFSHR 适用: 人, 大鼠, 猴, Cow, Pig, Bat, 山羊, 马, 兔, 绵羊 IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Follicle Stimulating Hormone Receptor (FSHR) antibodyFSHR 适用: 人 IHC, StM 宿主: 小鼠 Monoclonal FSHR-1400 unconjugated
anti-Follicle Stimulating Hormone Receptor (FSHR) antibodyFSHR 适用: 人 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-Follicle Stimulating Hormone Receptor (FSHR) (AA 278-327) antibodyFSHR 适用: 人 IHC, ELISA, IF, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Follicle Stimulating Hormone Receptor (FSHR) (AA 414-438), (Middle Region) antibodyFSHR 适用: 人, 小鼠 WB 宿主: 兔 Polyclonal unconjugated
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- 应用备注
- Approved: IHC, IHC-P (10 - 20 μg/mL)
- 说明
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Target Species of Antibody: Human
- 实验流程
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The IHC-pro Immunohistochemistry Protocol
Tissue Preparation
Formalin fixation and embedding in paraffin wax
Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.
Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.
Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.
Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.
Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip - 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- Lot specific
- 缓冲液
- PBS, less than 0.1 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
- 有效期
- 12 months
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- 抗原
- FSHR (Follicle Stimulating Hormone Receptor (FSHR))
- 别名
- FSH Receptor / FSHR (FSHR 产品)
- 别名
- FSHRO antibody, LGR1 antibody, ODG1 antibody, FSH-R antibody, eFSHR antibody, FSHRE antibody, fshr antibody, FSHR antibody, fshr-A antibody, GTHR-I antibody, zfGTHR-I antibody, LOC100304505 antibody, follicle stimulating hormone receptor antibody, follicle stimulating hormone receptor L homeolog antibody, follicle-stimulating hormone receptor antibody, FSHR antibody, fshr antibody, Fshr antibody, fshr.L antibody, Bm1_17785 antibody, LOC100304505 antibody
- 背景
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Name/Gene ID: FSHR
Subfamily: Glycoprotein hormone
Family: GPCR
Synonyms: FSHR, Follitropin receptor, FSHRO, Ovarian dysgenesis 1, FSH receptor, FSH-R, LGR1, ODG1 - 基因ID
- 2492
- 途径
- Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Regulation of Hormone Metabolic Process, Platelet-derived growth Factor Receptor Signaling
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