FZD2 抗体 (N-Term)
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- 抗原 See all FZD2 抗体
- FZD2 (Frizzled Family Receptor 2 (FZD2))
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抗原表位
- N-Term
- 适用
- 人, 大鼠, 小鼠, 马, Cow, 犬, Hamster, Pig, 猴, 兔
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This FZD2 antibody is un-conjugated
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应用范围
- Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 品牌
- IHC-plus™
- 特异性
- Human Frizzled 2. BLAST analysis of the peptide immunogen showed no homology with other human proteins, except FZD7 (60 % ), DMAP1 (40 %).
- 预测反应
- Percent identity with other species by BLAST analysis: Human, Gorilla, Monkey, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bovine, Horse, Rabbit, Pig, Platypus (100%) Opossum (95%) Turkey, Lizard, Newt (80%).
- 纯化方法
- Immunoaffinity purified
- 免疫原
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Synthetic 20 amino acid peptide from N-terminal extracellular domain of human Frizzled 2. Percent identity with other species by BLAST analysis: Human, Gorilla, Monkey, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bovine, Horse, Rabbit, Pig, Platypus (100%), Opossum (95%), Turkey, Lizard, Newt (80%).
Type of Immunogen: Synthetic peptide - Top Product
- Discover our top product FZD2 Primary Antibody
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anti-Frizzled Family Receptor 2 (FZD2) (Internal Region) antibody
Verified FZD2 适用: 人 WB, ELISA, IF, IHC 宿主: 山羊 Polyclonal unconjugated
anti-Frizzled Family Receptor 2 (FZD2) (Extracellular Domain) antibodyFZD2 适用: 人, 大鼠, 小鼠, 马, Cow, 犬, Hamster, Pig IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Frizzled Family Receptor 2 (FZD2) (AA 192-245) antibodyFZD2 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 2C8 unconjugated
anti-Frizzled Family Receptor 2 (FZD2) (Internal Region) antibodyFZD2 适用: 人, 大鼠, 小鼠 WB, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Frizzled Family Receptor 2 (FZD2) (N-Term) antibodyFZD2 适用: 人, 大鼠, 小鼠, 马, Cow, 犬 WB 宿主: 兔 Polyclonal unconjugated
anti-Frizzled Family Receptor 2 (FZD2) antibodyFZD2 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Frizzled Family Receptor 2 (FZD2) (N-Term) antibodyFZD2 适用: 人, 大鼠, 小鼠, 马, Cow, 犬, 猴 WB 宿主: 兔 Polyclonal unconjugated
anti-Frizzled Family Receptor 2 (FZD2) (AA 201-250) antibodyFZD2 适用: 人, 大鼠, 小鼠 WB, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Frizzled Family Receptor 2 (FZD2) (AA 192-205), (Extracellular), (N-Term) antibodyFZD2 适用: 人, 大鼠 WB 宿主: 兔 Polyclonal unconjugated
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- 应用备注
- Approved: IHC, IHC-P (10 - 20 μg/mL)
- 说明
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Target Species of Antibody: Human
- 实验流程
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The IHC-pro Immunohistochemistry Protocol
Tissue Preparation
Formalin fixation and embedding in paraffin wax
Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.
Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.
Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.
Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.
Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip - 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- Lot specific
- 缓冲液
- PBS, less than 0.1 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
- 有效期
- 12 months
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- 抗原
- FZD2 (Frizzled Family Receptor 2 (FZD2))
- 别名
- FSD2 / Frizzled 2 (FZD2 产品)
- 别名
- BG01711 antibody, CG9739 antibody, D-Fz2 antibody, D-fz2 antibody, DFZ2 antibody, DFz-2 antibody, DFz2 antibody, Dfrz2 antibody, Dfz2 antibody, Dfz[[2]] antibody, Dm Fz2 antibody, Dmel\\CG9739 antibody, FZ2 antibody, Fz2 antibody, dFz2 antibody, dfz(2) antibody, dfz2 antibody, dfz2r antibody, frz2 antibody, fz-2 antibody, fz8 antibody, zfz2 antibody, zg08 antibody, cb383 antibody, wu:fb70g09 antibody, FZD2 antibody, fzE2 antibody, hFz2 antibody, Fz-10 antibody, Fzd10 antibody, cFz-2 antibody, frizzled2 antibody, fz2 antibody, xfz2 antibody, AL033370 antibody, AW456835 antibody, Fz10 antibody, Mfz10 antibody, Mfz10a antibody, frizzled 2 antibody, frizzled class receptor 2 antibody, frizzled class receptor 2 S homeolog antibody, fz2 antibody, fzd2 antibody, FZD2 antibody, Fzd2 antibody, fzd2.S antibody
- 背景
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Name/Gene ID: FZD2
Subfamily: Frizzled
Family: GPCR
Synonyms: FZD2, Dfrizzled2, Fz2, Frizzled family receptor 2, Frizzled-2, HFz2, Frizzled homolog 2, Fz-2, FzE2, Mfz10, Rfz2, Frizzled 2 - 基因ID
- 2535
- 途径
- WNT signaling
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