ESRRG 抗体 (C-Term)
-
- 抗原 See all ESRRG 抗体
- ESRRG (Estrogen-Related Receptor gamma (ESRRG))
-
抗原表位
- C-Term
-
适用
- 人, 小鼠, 大鼠, 犬, 兔, Cow, 马, 猴, 小鸡, Pig, Hamster
-
宿主
- 兔
-
克隆类型
- 多克隆
-
标记
- This ESRRG antibody is un-conjugated
-
应用范围
- Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Chromatin Immunoprecipitation (ChIP)
- 品牌
- IHC-plus™
- 特异性
- Human ESRRG / ERR3. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
- 预测反应
- Percent identity with other species by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bovine, Horse, Rabbit, Pig, Opossum, Turkey, Chicken, Platypus, Lizard (100%) Bat, Xenopus, Zebrafish (93%) Stickleback, Medaka, Pufferfish (87%).
- 纯化方法
- Immunoaffinity purified
- 免疫原
-
Synthetic 15 amino acid peptide from C-terminus of human ESRRG / ERR3. Percent identity with other species by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bovine, Horse, Rabbit, Pig, Opossum, Turkey, Chicken, Platypus, Lizard (100%), Bat, Xenopus, Zebrafish (93%), Stickleback, Medaka, Pufferfish (87%).
Type of Immunogen: Synthetic peptide - Top Product
- Discover our top product ESRRG Primary Antibody
-
anti-Estrogen-Related Receptor gamma (ESRRG) (AA 24-263) antibody
ESRRG 适用: 人 WB, IHC, IHC (p) 宿主: 小鼠 Monoclonal 6G1 unconjugated
anti-Estrogen-Related Receptor gamma (ESRRG) (Middle Region) antibodyESRRG 适用: 人, 小鼠, 大鼠, 犬, 兔, Cow, 马, 斑马鱼, 豚鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Estrogen-Related Receptor gamma (ESRRG) antibodyESRRG 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Estrogen-Related Receptor gamma (ESRRG) antibodyESRRG 适用: 人 WB, IHC (wm) 宿主: 兔 Polyclonal unconjugated
anti-Estrogen-Related Receptor gamma (ESRRG) (N-Term) antibodyESRRG 适用: 人, 小鼠, 大鼠, 犬, 兔, Cow, 马, 猴, Bat, Hamster ELISA, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Estrogen-Related Receptor gamma (ESRRG) (N-Term) antibodyESRRG 适用: 人, 小鼠, 大鼠, 犬, 兔, Cow, 马, 斑马鱼, 猴, Bat, 小鸡, 非洲爪蟾, Hamster IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Estrogen-Related Receptor gamma (ESRRG) (AA 190-239) antibodyESRRG 适用: 人, 小鼠, 大鼠, 犬, 兔, Cow, 马, 斑马鱼, 豚鼠, 猴, Bat, 小鸡, 非洲爪蟾, Pig WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Estrogen-Related Receptor gamma (ESRRG) (Internal Region) antibodyVerified ESRRG 适用: 人 WB, ELISA 宿主: 山羊 Polyclonal unconjugated
anti-Estrogen-Related Receptor gamma (ESRRG) (AA 24-263) antibodyESRRG 适用: 人 WB, IF 宿主: 小鼠 Monoclonal 1D10 unconjugated
anti-Estrogen-Related Receptor gamma (ESRRG) (AA 302-458) antibodyESRRG 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
-
- 应用备注
- Approved: ChrIP, IHC, IHC-P (9 - 17 μg/mL), WB
- 说明
-
Target Species of Antibody: Human
- 实验流程
-
The IHC-pro Immunohistochemistry Protocol
Tissue Preparation
Formalin fixation and embedding in paraffin wax
Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.
Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.
Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.
Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.
Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip - 限制
- 仅限研究用
-
- 状态
- Liquid
- 浓度
- Lot specific
- 缓冲液
- PBS, less than 0.1 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
- 有效期
- 12 months
-
- 抗原
- ESRRG (Estrogen-Related Receptor gamma (ESRRG))
- 别名
- ESRRG / ERR gamma (ESRRG 产品)
- 别名
- ERR3 antibody, ERRgamma antibody, NR3B3 antibody, ESRRG antibody, USH2A antibody, DKFZp459J0417 antibody, err3 antibody, nr3b3 antibody, LOC100219115 antibody, errg antibody, errgamma antibody, esrrg antibody, errb/g antibody, esrrgl antibody, errbeta/gamma antibody, Errg antibody, mKIAA0832 antibody, estrogen related receptor gamma antibody, estrogen-related receptor gamma antibody, estrogen-related receptor gamma a antibody, estrogen-related receptor gamma b antibody, ESRRG antibody, esrrg antibody, Esrrg antibody, esrrga antibody, esrrgb antibody
- 背景
-
Name/Gene ID: ESRRG
Subfamily: NR3 Steroid receptor
Family: NHR
Synonyms: ESRRG, ERR Gamma, ERR gamma-2, ERR3, Err-gamma, NR3B3, ERRG2, ERRgamma, KIAA0832 - 基因ID
- 2104
- 途径
- Nuclear Receptor Transcription Pathway, Retinoic Acid Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway
-
