ESRRB 抗体 (Ligand Binding Domain)
-
- 抗原 See all ESRRB 抗体
- ESRRB (Estrogen-Related Receptor beta (ESRRB))
-
抗原表位
- Ligand Binding Domain
-
适用
- 人, 马, 犬, Cow, 兔, 猴, Pig, Hamster
-
宿主
- 兔
-
克隆类型
- 多克隆
-
标记
- This ESRRB antibody is un-conjugated
-
应用范围
- Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 品牌
- IHC-plus™
- 特异性
- Human ESRRB. BLAST analysis of the peptide immunogen showed no homology with other human proteins, except ESRRG (67 % ).
- 预测反应
- Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Hamster, Panda, Dog, Bovine, Horse, Rabbit, Pig, Opossum (100%) Mouse, Rat, Elephant, Turkey, Chicken, Lizard (93%) Bat (87%) Stickleback, Medaka, Pufferfish, Zebrafish (80%).
- 纯化方法
- Immunoaffinity purified
- 免疫原
-
Synthetic 15 amino acid peptide from ligand-binding domain of human ESRRB. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Hamster, Panda, Dog, Bovine, Horse, Rabbit, Pig, Opossum (100%), Mouse, Rat, Elephant, Turkey, Chicken, Lizard (93%), Bat (87%), Stickleback, Medaka, Pufferfish, Zebrafish (80%).
Type of Immunogen: Synthetic peptide - Top Product
- Discover our top product ESRRB Primary Antibody
-
anti-Estrogen-Related Receptor beta (ESRRB) (AA 275-305) antibody
ESRRB 适用: 人 WB, IHC (p), FACS 宿主: 兔 Polyclonal RB17738 unconjugated
anti-Estrogen-Related Receptor beta (ESRRB) (AA 166-438) antibodyESRRB 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Estrogen-Related Receptor beta (ESRRB) (Internal Region) antibodyESRRB 适用: 人, 马, 猴, Hamster IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Estrogen-Related Receptor beta (ESRRB) (N-Term) antibodyESRRB 适用: 人, 马, 猴 IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Estrogen-Related Receptor beta (ESRRB) (AA 283-312), (Middle Region) antibodyESRRB 适用: 人 WB, IHC (p), FACS, EIA 宿主: 兔 Polyclonal unconjugated
anti-Estrogen-Related Receptor beta (ESRRB) (AA 1-100) antibodyESRRB 适用: 小鼠 WB, ELISA, IHC (p), IF (cc), IF (p), ICC, IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Estrogen-Related Receptor beta (ESRRB) (AA 432-502) antibodyESRRB 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Estrogen-Related Receptor beta (ESRRB) (N-Term) antibodyESRRB 适用: 人, 小鼠, 大鼠, 马, 犬, Cow, 豚鼠, 猴, Pig, Bat WB 宿主: 兔 Polyclonal unconjugated
anti-Estrogen-Related Receptor beta (ESRRB) (AA 38-87) antibodyESRRB 适用: 人, 马, 犬, Cow, 豚鼠, 猴, Pig, Bat WB 宿主: 兔 Polyclonal unconjugated
anti-Estrogen-Related Receptor beta (ESRRB) (N-Term) antibodyESRRB 适用: 人, 小鼠, 大鼠, 马, 犬, Cow, 豚鼠, 兔 WB 宿主: 兔 Polyclonal unconjugated
-
- 应用备注
- Approved: IHC, IHC-P (3 μg/mL)
- 说明
-
Target Species of Antibody: Human
- 实验流程
-
The IHC-pro Immunohistochemistry Protocol
Tissue Preparation
Formalin fixation and embedding in paraffin wax
Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.
Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.
Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.
Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.
Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip - 限制
- 仅限研究用
-
- 状态
- Liquid
- 浓度
- Lot specific
- 缓冲液
- PBS, less than 0.1 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
- 有效期
- 12 months
-
- 抗原
- ESRRB (Estrogen-Related Receptor beta (ESRRB))
- 别名
- ESRRB / ERR beta (ESRRB 产品)
- 别名
- errb antibody, errbeta antibody, ESRRB antibody, Err2 antibody, Errb antibody, Estrrb antibody, Nr3b2 antibody, DFNB35 antibody, ERR2 antibody, ERRb antibody, ESRL2 antibody, NR3B2 antibody, estrogen-related receptor beta antibody, estrogen related receptor beta antibody, estrogen related receptor, beta antibody, esrrb antibody, ESRRB antibody, Esrrb antibody
- 背景
-
Name/Gene ID: ESRRB
Subfamily: NR3 Steroid receptor
Family: NHR
Synonyms: ESRRB, DFNB35, ERRb, ERRbeta-2, ESRL2, Estrogen receptor-like 2, ERR-beta, ERRB2, ERRbeta, NR3B2, Nuclear receptor ERRB2, Orphan nuclear receptor, ERR beta-2, ERR2, Estrogen-related receptor 2, Estrogen-related receptor beta, Steroid hormone receptor ERR2 - 基因ID
- 2103
- 途径
- Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway, Stem Cell Maintenance
-
