EDNRB 抗体 (N-Term)
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- 抗原 See all EDNRB 抗体
- EDNRB (Endothelin Receptor Type B (EDNRB))
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抗原表位
- N-Term
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适用
- 人, 犬
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This EDNRB antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 品牌
- IHC-plus™
- 特异性
- Human EDNRB. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
- 预测反应
- Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Dog (100%) Marmoset (83%).
- 纯化方法
- Immunoaffinity purified
- 免疫原
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Synthetic 18 amino acid peptide from N-terminal extracellular domain of human EDNRB. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Dog (100%), Marmoset (83%).
Type of Immunogen: Synthetic peptide - Top Product
- Discover our top product EDNRB Primary Antibody
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- 应用备注
- Approved: IHC, IHC-P (16 μg/mL), WB
- 说明
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Target Species of Antibody: Human
- 实验流程
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The IHC-pro Immunohistochemistry Protocol
Tissue Preparation
Formalin fixation and embedding in paraffin wax
Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.
Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.
Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.
Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.
Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip - 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- Lot specific
- 缓冲液
- PBS, less than 0.1 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
- 有效期
- 12 months
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- 抗原
- EDNRB (Endothelin Receptor Type B (EDNRB))
- 别名
- EDNRB / Endothelin B Receptor (EDNRB 产品)
- 别名
- ABCDS antibody, ET-B antibody, ET-BR antibody, ETB antibody, ETBR antibody, ETRB antibody, HSCR antibody, HSCR2 antibody, WS4A antibody, Ednra antibody, Etb antibody, ETR-b antibody, ETb antibody, Sox10m1 antibody, EDNRB antibody, abcds antibody, etb antibody, etrb antibody, hscr antibody, hscr2 antibody, endothelin receptor type B antibody, EDNRB antibody, Ednrb antibody, ednrb antibody
- 背景
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Name/Gene ID: EDNRB
Subfamily: Endothelin
Family: GPCR
Synonyms: EDNRB, ABCDS, Endothelin B receptor, Etb receptor, ET-B, Et-b receptor, Et-b-r, ET-RB, ETBR, ETRB, Etb-svr, WS4A, Endothelin receptor type B, ET-BR, ETB, HSCR, HSCR2 - 基因ID
- 1910
- 途径
- Cellular Response to Molecule of Bacterial Origin, cAMP Metabolic Process
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