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- 抗原 See all LPAR1 抗体
- LPAR1 (Lysophosphatidic Acid Receptor 1 (LPAR1))
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抗原表位
- N-Term
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适用
- 人, 猴
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This LPAR1 antibody is un-conjugated
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应用范围
- Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 品牌
- IHC-plus™
- 特异性
- Human EDG2. BLAST analysis of the peptide immunogen showed no homology with other human proteins, except DDX60 (53 % ), ACER3 (47 %).
- 预测反应
- Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Elephant (100%) Mouse, Rat, Sheep, Dog, Bovine, Bat, Hamster, Panda, Horse, Rabbit, Pig, Opossum, Turkey, Chicken, Platypus (93%) Xenopus, Pufferfish, Zebrafish (87%).
- 纯化方法
- Immunoaffinity purified
- 免疫原
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Synthetic 15 amino acid peptide from N-terminal extracellular domain of human EDG2. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Elephant (100%), Mouse, Rat, Sheep, Dog, Bovine, Bat, Hamster, Panda, Horse, Rabbit, Pig, Opossum, Turkey, Chicken, Platypus (93%), Xenopus, Pufferfish, Zebrafish (87%).
Type of Immunogen: Synthetic peptide - Top Product
- Discover our top product LPAR1 Primary Antibody
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anti-Lysophosphatidic Acid Receptor 1 (LPAR1) (AA 281-364) antibody
LPAR1 适用: 人, 小鼠 WB, ELISA, IF (p), IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Lysophosphatidic Acid Receptor 1 (LPAR1) antibodyLPAR1 适用: 人 WB, IF, FACS 宿主: 小鼠 Monoclonal 1G6 unconjugated
anti-Lysophosphatidic Acid Receptor 1 (LPAR1) (N-Term) antibodyLPAR1 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Lysophosphatidic Acid Receptor 1 (LPAR1) (AA 316-364) antibodyLPAR1 适用: 人 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Lysophosphatidic Acid Receptor 1 (LPAR1) (Cytoplasmic Domain) antibodyLPAR1 适用: 人, 小鼠, 大鼠, 猴, Bat, 小鸡, Cow, 犬, Hamster, 马, Pig, 绵羊, 非洲爪蟾, 兔 IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Lysophosphatidic Acid Receptor 1 (LPAR1) (N-Term) antibodyLPAR1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Lysophosphatidic Acid Receptor 1 (LPAR1) (AA 342-359) antibodyLPAR1 适用: 人, 小鼠, 大鼠, 猴, Bat, 小鸡, Cow, 犬, Hamster, 马, Pig, 绵羊, 非洲爪蟾 WB, IHC, IHC (p), ICC 宿主: 兔 Polyclonal unconjugated
anti-Lysophosphatidic Acid Receptor 1 (LPAR1) antibodyLPAR1 适用: 人 WB, ELISA 宿主: 兔 Monoclonal 4C3 unconjugated Recombinant Antibody
anti-Lysophosphatidic Acid Receptor 1 (LPAR1) (AA 5-54) antibodyLPAR1 适用: 人, 大鼠 WB, ELISA, IHC, IF, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Lysophosphatidic Acid Receptor 1 (LPAR1) antibodyLPAR1 适用: 人 WB 宿主: 兔 Monoclonal unconjugated
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- 应用备注
- Approved: IHC, IHC-P (1 - 2 μg/mL)
- 说明
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Target Species of Antibody: Human
- 实验流程
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The IHC-pro Immunohistochemistry Protocol
Tissue Preparation
Formalin fixation and embedding in paraffin wax
Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.
Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.
Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.
Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.
Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip - 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- Lot specific
- 缓冲液
- PBS, less than 0.1 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
- 有效期
- 12 months
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- 抗原
- LPAR1 (Lysophosphatidic Acid Receptor 1 (LPAR1))
- 别名
- LPAR1 / LPA1 / EDG2 (LPAR1 产品)
- 别名
- EDG2 antibody, GPR26 antibody, Gpcr26 antibody, LPA1 antibody, Mrec1.3 antibody, VZG1 antibody, edg-2 antibody, rec.1.3 antibody, vzg-1 antibody, Edg2 antibody, EDG-2 antibody, 5031439C20 antibody, AI326300 antibody, Kdt2 antibody, lpA1 antibody, edg2 antibody, si:dz22b13.1 antibody, si:dz46m7.1 antibody, zgc:92157 antibody, LPA receptor 1-A antibody, LPA-1-A antibody, gpr26 antibody, lpa1 antibody, lpa1r antibody, lpa1r1 antibody, lpar1 antibody, lpar1-a antibody, lpar1-b antibody, vzg1 antibody, xLPA1-1 antibody, LPA receptor 1-B antibody, LPA-1-B antibody, edg2-A antibody, xLPA1-2 antibody, xlpa1R antibody, lysophosphatidic acid receptor 1 antibody, lysophosphatidic acid receptor 1 S homeolog antibody, lysophosphatidic acid receptor 1 L homeolog antibody, LPAR1 antibody, Lpar1 antibody, lpar1 antibody, lpar1.S antibody, lpar1.L antibody
- 背景
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Name/Gene ID: LPAR1
Subfamily: Lysophospholipid/Lysosphingolipid
Family: GPCR
Synonyms: LPAR1, EDG-2, EDG2, Gpcr26, Rec.1.3, Vzg-1, Ventricular zone gene 1, LPA receptor 1, LPA-1, LPA1, Mrec1.3, VZG1 - 基因ID
- 1902
- 途径
- Myometrial Relaxation and Contraction, Smooth Muscle Cell Migration
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