DUSP22 抗体 (N-Term)
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- 抗原 See all DUSP22 抗体
- DUSP22 (Dual Specificity Phosphatase 22 (DUSP22))
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抗原表位
- N-Term
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适用
- 人, 兔, Cow, 马, 猴
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This DUSP22 antibody is un-conjugated
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应用范围
- Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 品牌
- IHC-plus™
- 特异性
- Human DUSP22. BLAST analysis of the peptide immunogen showed no homology with other human proteins, except DUSP15 (59 % ).
- 预测反应
- Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Bovine, Horse, Rabbit (100%) Mouse, Rat (94%) Elephant, Chicken (88%) Xenopus (82%).
- 纯化方法
- Immunoaffinity purified
- 免疫原
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Synthetic 17 amino acid peptide from near N-terminus of human DUSP22. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Bovine, Horse, Rabbit (100%), Mouse, Rat (94%), Elephant, Chicken (88%), Xenopus (82%).
Type of Immunogen: Synthetic peptide - Top Product
- Discover our top product DUSP22 Primary Antibody
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anti-Dual Specificity Phosphatase 22 (DUSP22) (AA 1-184) antibody
DUSP22 适用: 人 WB, IF, IP 宿主: 兔 Polyclonal unconjugated
anti-Dual Specificity Phosphatase 22 (DUSP22) (AA 112-184) antibodyDUSP22 适用: 人 WB, ELISA, RNAi 宿主: 小鼠 Monoclonal 3D3 unconjugated
anti-Dual Specificity Phosphatase 22 (DUSP22) (AA 1-184) antibodyDUSP22 适用: 人 WB, IF 宿主: 小鼠 Polyclonal unconjugated
anti-Dual Specificity Phosphatase 22 (DUSP22) (AA 1-184) antibodyDUSP22 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Dual Specificity Phosphatase 22 (DUSP22) (Internal Region) antibodyDUSP22 适用: 人, 小鼠 WB, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Dual Specificity Phosphatase 22 (DUSP22) antibodyDUSP22 适用: 人, 小鼠 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
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- 应用备注
- Approved: IHC, IHC-P (2 - 22 μg/mL)
- 说明
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Target Species of Antibody: Human
- 实验流程
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The IHC-pro Immunohistochemistry Protocol
Tissue Preparation
Formalin fixation and embedding in paraffin wax
Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.
Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.
Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.
Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.
Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip - 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- Lot specific
- 缓冲液
- PBS, less than 0.1 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
- 有效期
- 12 months
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- 抗原
- DUSP22 (Dual Specificity Phosphatase 22 (DUSP22))
- 别名
- DUSP22 / JSP 1 (DUSP22 产品)
- 别名
- dusp22 antibody, wu:fa11e09 antibody, zgc:92816 antibody, vhx antibody, jkap antibody, jsp1 antibody, mkpx antibody, DUSP22 antibody, ch73-170d1.1 antibody, zgc:112536 antibody, JKAP antibody, JSP1 antibody, LMWDSP2 antibody, MKPX antibody, VHX antibody, 1110028K04Rik antibody, AW212235 antibody, dual specificity phosphatase 22 antibody, dual specificity phosphatase 22a antibody, dual specificity phosphatase 22b antibody, DUSP22 antibody, dusp22a antibody, dusp22 antibody, dusp22b antibody, Dusp22 antibody
- 背景
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Name/Gene ID: DUSP22
Subfamily: LMW-DSP
Family: Protein Phosphatase
Synonyms: DUSP22, JSP 1, JKAP, JNK-stimulating phosphatase 1, LMW-DSP2, Map kinase phosphatase x, MKPX, VHX, JNK-stimulatory phosphatase-1, JSP-1, JSP1, LMWDSP2, MKP-X - 基因ID
- 56940
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