Dopamine Receptor d1 抗体 (Extracellular Domain)
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- 抗原 See all Dopamine Receptor d1 (DRD1) 抗体
- Dopamine Receptor d1 (DRD1) (Dopamine Receptor D1 (DRD1))
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抗原表位
- Extracellular Domain
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适用
- 人
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This Dopamine Receptor d1 antibody is un-conjugated
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应用范围
- Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 品牌
- IHC-plus™
- 特异性
- Human DRD1. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
- 预测反应
- Percent identity with other species by BLAST analysis: Human, Gorilla, Monkey (100%) Gibbon, Marmoset (95%) Elephant (90%) Dog, Rabbit (85%) Horse (80%).
- 纯化方法
- Immunoaffinity purified
- 免疫原
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Synthetic 20 amino acid peptide from 2nd extracellular domain of human DRD1. Percent identity with other species by BLAST analysis: Human, Gorilla, Monkey (100%), Gibbon, Marmoset (95%), Elephant (90%), Dog, Rabbit (85%), Horse (80%).
Type of Immunogen: Synthetic peptide - Top Product
- Discover our top product DRD1 Primary Antibody
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anti-Dopamine Receptor D1 (DRD1) (AA 338-446) antibody
DRD1 适用: 人 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-Dopamine Receptor D1 (DRD1) (AA 101-200) antibodyDRD1 适用: 人, 大鼠, 小鼠 WB, ELISA, FACS, IF (cc), IF (p), ICC, IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Dopamine Receptor D1 (DRD1) (AA 11-100) antibodyDRD1 适用: 人, 大鼠 WB, ELISA, FACS, IF (cc), IF (p), ICC, IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Dopamine Receptor D1 (DRD1) (AA 338-446) antibodyDRD1 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Dopamine Receptor D1 (DRD1) (N-Term) antibodyDRD1 适用: 人, 大鼠, 小鼠 WB, ELISA, IHC, ICC, IF 宿主: 兔 Polyclonal unconjugated
anti-Dopamine Receptor D1 (DRD1) (C-Term) antibodyDRD1 适用: 人 WB, ICC, IF, IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Dopamine Receptor D1 (DRD1) (AA 338-446) antibodyDRD1 适用: 大鼠 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-Dopamine Receptor D1 (DRD1) (C-Term) antibodyDRD1 适用: 人, 猴 IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Dopamine Receptor D1 (DRD1) (C-Term) antibodyDRD1 适用: 人, 猴, 兔 ELISA, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Dopamine Receptor D1 (DRD1) (AA 372-385), (C-Term), (Intracellular) antibodyDRD1 适用: 大鼠, 小鼠 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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Approved: IHC, IHC-P (10 μg/mL)
Not recommended for: ICC, IHC-Fr - 说明
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Target Species of Antibody: Human
- 实验流程
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The IHC-pro Immunohistochemistry Protocol
Tissue Preparation
Formalin fixation and embedding in paraffin wax
Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.
Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.
Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.
Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.
Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip - 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- Lot specific
- 缓冲液
- PBS, less than 0.1 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
- 有效期
- 12 months
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- 抗原
- Dopamine Receptor d1 (DRD1) (Dopamine Receptor D1 (DRD1))
- 别名
- DRD1 / Dopamine Receptor D1 (DRD1 产品)
- 别名
- DRD1 antibody, Dop1 antibody, D1A antibody, DADR antibody, DRD1A antibody, drd1 antibody, C030036C15Rik antibody, Drd-1 antibody, Drd1 antibody, Gpcr15 antibody, D1a antibody, Drd1a antibody, dopamine receptor D1 antibody, dopamine receptor, D1 antibody, dopamine receptor D1b antibody, DRD1 antibody, Dop1 antibody, drd1b antibody, Drd1 antibody
- 背景
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Name/Gene ID: DRD1
Subfamily: Dopamine
Family: GPCR
Synonyms: DRD1, D(1A) dopamine receptor, D1a dopamine receptor, Dopamine d-1 receptor, Dopamine receptor d1a, DRD1A, DADR, Dopamine d1a receptor, Dopamine receptor D1, Dopamine 1 receptor, Dopamine type 1 receptor, D-1 dopamine receptor, D1 dopamine receptor, D1a receptor, Dopamine D1 receptor - 基因ID
- 1812
- 途径
- cAMP Metabolic Process, Inositol Metabolic Process, Protein targeting to Nucleus, Feeding Behaviour, Smooth Muscle Cell Migration, Regulation of long-term Neuronal Synaptic Plasticity
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