DPP9 抗体 (Internal Region)
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- 抗原 See all DPP9 抗体
- DPP9 (Dipeptidyl-Peptidase 9 (DPP9))
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抗原表位
- Internal Region
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适用
- 人, 小鼠, Hamster
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This DPP9 antibody is un-conjugated
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应用范围
- Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 品牌
- IHC-plus™
- 特异性
- Human DPP9. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
- 预测反应
- Percent identity with other species by BLAST analysis: Human, Gorilla, Monkey, Mouse, Hamster (100%) Marmoset, Elephant, Bovine, Bat (93%) Panda, Horse (86%).
- 纯化方法
- Immunoaffinity purified
- 免疫原
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Synthetic 14 amino acid peptide from internal region of human DPP9. Percent identity with other species by BLAST analysis: Human, Gorilla, Monkey, Mouse, Hamster (100%), Marmoset, Elephant, Bovine, Bat (93%), Panda, Horse (86%).
Type of Immunogen: Synthetic peptide - Top Product
- Discover our top product DPP9 Primary Antibody
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anti-Dipeptidyl-Peptidase 9 (DPP9) antibody
DPP9 适用: 人, 猴, 犬 WB, IHC, FACS, IHC (p) 宿主: 小鼠 Monoclonal 4F1 unconjugated
anti-Dipeptidyl-Peptidase 9 (DPP9) (N-Term) antibodyDPP9 适用: 人, 猴, Cow IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Dipeptidyl-Peptidase 9 (DPP9) antibodyDPP9 适用: 人, 小鼠, 猴, 大鼠 WB 宿主: 小鼠 Monoclonal 4C10 unconjugated
anti-Dipeptidyl-Peptidase 9 (DPP9) (AA 289-437) antibodyDPP9 适用: 人 IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Dipeptidyl-Peptidase 9 (DPP9) (AA 1-863) antibodyDPP9 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-Dipeptidyl-Peptidase 9 (DPP9) antibodyDPP9 适用: 人, 犬 WB, IHC, FACS, IHC (p) 宿主: 小鼠 Monoclonal 6A12 unconjugated
anti-Dipeptidyl-Peptidase 9 (DPP9) antibodyDPP9 适用: 人 WB, IHC, FACS, IHC (p), IF 宿主: 小鼠 Monoclonal 4H8 unconjugated
anti-Dipeptidyl-Peptidase 9 (DPP9) antibodyDPP9 适用: 人, 小鼠 WB, IHC, FACS, IHC (p) 宿主: 小鼠 Monoclonal 2E3 unconjugated
anti-Dipeptidyl-Peptidase 9 (DPP9) antibodyDPP9 适用: 人, 猴, 犬, 大鼠 WB, IHC, FACS, IHC (p) 宿主: 小鼠 Monoclonal 1G9 unconjugated
anti-Dipeptidyl-Peptidase 9 (DPP9) (AA 1-100) antibodyDPP9 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
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- 应用备注
- Approved: IHC, IHC-P (10 μg/mL)
- 说明
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Target Species of Antibody: Human
- 实验流程
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The IHC-pro Immunohistochemistry Protocol
Tissue Preparation
Formalin fixation and embedding in paraffin wax
Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.
Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.
Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.
Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.
Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip - 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- Lot specific
- 缓冲液
- PBS, less than 0.1 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
- 有效期
- 12 months
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- 抗原
- DPP9 (Dipeptidyl-Peptidase 9 (DPP9))
- 别名
- DPP9 (DPP9 产品)
- 别名
- DP9 antibody, DPLP9 antibody, DPRP-2 antibody, DPRP2 antibody, 6430584G11Rik antibody, A330078I11 antibody, DPP IX antibody, im:7145318 antibody, zgc:152900 antibody, dpp9 antibody, dipeptidyl peptidase 9 antibody, dipeptidylpeptidase 9 antibody, dipeptidyl-peptidase 9 antibody, dipeptidyl-peptidase 9 L homeolog antibody, DPP9 antibody, Dpp9 antibody, dpp9 antibody, dpp9.L antibody
- 背景
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Name/Gene ID: DPP9
Subfamily: Serine S9B
Family: Protease
Synonyms: DPP9, Dipeptidyl-peptidase 9, DPRP-2, Dipeptidyl peptidase IX, DP9, DPP IX, DPLP9, Dipeptidyl peptidase 9, Dipeptidylpeptidase 9, DPRP2 - 基因ID
- 91039
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