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- 抗原 See all DPP8 抗体
- DPP8 (Dipeptidyl-Peptidase 8 (DPP8))
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抗原表位
- Internal Region
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适用
- 人, 猴
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This DPP8 antibody is un-conjugated
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应用范围
- Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 品牌
- IHC-plus™
- 特异性
- Human DPP8. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
- 预测反应
- Percent identity with other species by BLAST analysis: Human, Gorilla, Monkey, Marmoset (100%) Mouse, Elephant, Panda, Dog, Bovine, Bat, Horse, Rabbit, Opossum (93%) Rat, Pig (86%).
- 纯化方法
- Immunoaffinity purified
- 免疫原
- Synthetic 14 amino acid peptide from internal region of human DPP8 (NP_569118.1). Percent identity with other species by BLAST analysis: Human, Gorilla, Monkey, Marmoset (100%), Mouse, Elephant, Panda, Dog, Bovine, Bat, Horse, Rabbit, Opossum (93%), Rat, Pig (86%).
- Top Product
- Discover our top product DPP8 Primary Antibody
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anti-Dipeptidyl-Peptidase 8 (DPP8) antibody
DPP8 适用: 人, 小鼠, 大鼠, 犬 WB, IHC, IHC (p) 宿主: 小鼠 Monoclonal 1D2 unconjugated
anti-Dipeptidyl-Peptidase 8 (DPP8) (AA 10-230) antibodyDPP8 适用: 人 IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Dipeptidyl-Peptidase 8 (DPP8) (N-Term) antibodyDPP8 适用: 人, 猴, 犬, 兔, Pig, Bat, Cow, 马 IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Dipeptidyl-Peptidase 8 (DPP8) (N-Term) antibodyDPP8 适用: 人 WB, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Dipeptidyl-Peptidase 8 (DPP8) (AA 23-52), (N-Term) antibodyDPP8 适用: 人 WB, IHC (p), FACS 宿主: 兔 Polyclonal RB17830 unconjugated
anti-Dipeptidyl-Peptidase 8 (DPP8) (AA 846-876), (C-Term) antibodyDPP8 适用: 人 WB, IHC (p), FACS 宿主: 兔 Polyclonal RB19258 unconjugated
anti-Dipeptidyl-Peptidase 8 (DPP8) (AA 161-244) antibodyDPP8 适用: 人 ELISA 宿主: 小鼠 Monoclonal 1B10 unconjugated
anti-Dipeptidyl-Peptidase 8 (DPP8) (AA 1-898) antibodyDPP8 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-Dipeptidyl-Peptidase 8 (DPP8) (AA 349-662) antibodyDPP8 适用: 人, 小鼠 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Dipeptidyl-Peptidase 8 (DPP8) (AA 161-245) antibodyDPP8 适用: 人 IHC, ELISA, IHC (p) 宿主: 小鼠 Monoclonal unconjugated
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- 应用备注
- Approved: IHC, IHC-P (5 μg/mL)
- 说明
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Target Species of Antibody: Human
- 实验流程
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The IHC-pro Immunohistochemistry Protocol
Tissue Preparation
Formalin fixation and embedding in paraffin wax
Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.
Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.
Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.
Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.
Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip - 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- Lot specific
- 缓冲液
- PBS, less than 0.1 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
- 有效期
- 12 months
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- 抗原
- DPP8 (Dipeptidyl-Peptidase 8 (DPP8))
- 别名
- Dipeptidylpeptidase 8 / DPP8 (DPP8 产品)
- 别名
- DP8 antibody, DPRP1 antibody, MSTP141 antibody, dp8 antibody, dpp8b antibody, dprp1 antibody, MGC131071 antibody, DPP8 antibody, dpp8 antibody, dpp8a antibody, 2310004I03Rik antibody, 4932434F09Rik antibody, AI666706 antibody, DPP VIII antibody, dipeptidyl peptidase 8 antibody, dipeptidyl-peptidase 8 L homeolog antibody, dipeptidyl-peptidase 8 antibody, dipeptidyl-peptidase 8 S homeolog antibody, dipeptidylpeptidase 8 antibody, DPP8 antibody, dpp8.L antibody, dpp8 antibody, dpp8.S antibody, Dpp8 antibody
- 背景
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Name/Gene ID: DPP8
Subfamily: Serine S9B
Family: Protease
Synonyms: DPP8, Dipeptidyl-peptidase 8, DPRP1, Dipeptidyl peptidase VIII, Dipeptidylpeptidase 8, DPRP-1, Dipeptidyl peptidase 8, MSTP141, Prolyl dipeptidase DPP8, DP8, DPP VIII - 基因ID
- 54878
- NCBI登录号
- NP_569118
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