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- 抗原 See all DPP8 抗体
- DPP8 (Dipeptidyl-Peptidase 8 (DPP8))
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抗原表位
- Internal Region
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适用
- 人, 猴
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This DPP8 antibody is un-conjugated
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应用范围
- Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 品牌
- IHC-plus™
- 特异性
- Human DPP8. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
- 预测反应
- Percent identity with other species by BLAST analysis: Human, Gorilla, Monkey, Marmoset (100%) Mouse, Elephant, Panda, Dog, Bovine, Bat, Horse, Rabbit, Opossum (93%) Rat, Pig (86%).
- 纯化方法
- Immunoaffinity purified
- 免疫原
- Synthetic 14 amino acid peptide from internal region of human DPP8 (NP_569118.1). Percent identity with other species by BLAST analysis: Human, Gorilla, Monkey, Marmoset (100%), Mouse, Elephant, Panda, Dog, Bovine, Bat, Horse, Rabbit, Opossum (93%), Rat, Pig (86%).
- Top Product
- Discover our top product DPP8 Primary Antibody
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anti-Dipeptidyl-Peptidase 8 (DPP8) antibody
DPP8 适用: 人, 小鼠, 大鼠, 犬 WB, IHC, IHC (p) 宿主: 小鼠 Monoclonal 1D2 unconjugated
anti-Dipeptidyl-Peptidase 8 (DPP8) (AA 10-230) antibodyDPP8 适用: 人 IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Dipeptidyl-Peptidase 8 (DPP8) (N-Term) antibodyDPP8 适用: 人, 犬, 猴, 兔, Bat, Cow, 马, Pig IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Dipeptidyl-Peptidase 8 (DPP8) (N-Term) antibodyDPP8 适用: 人 WB, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Dipeptidyl-Peptidase 8 (DPP8) (AA 23-52), (N-Term) antibodyDPP8 适用: 人 WB, IHC (p), FACS 宿主: 兔 Polyclonal RB17830 unconjugated
anti-Dipeptidyl-Peptidase 8 (DPP8) (AA 846-876), (C-Term) antibodyDPP8 适用: 人 WB, IHC (p), FACS 宿主: 兔 Polyclonal RB19258 unconjugated
anti-Dipeptidyl-Peptidase 8 (DPP8) (AA 161-244) antibodyDPP8 适用: 人 ELISA 宿主: 小鼠 Monoclonal 1B10 unconjugated
anti-Dipeptidyl-Peptidase 8 (DPP8) (AA 1-898) antibodyDPP8 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-Dipeptidyl-Peptidase 8 (DPP8) (AA 349-662) antibodyDPP8 适用: 人, 小鼠 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Dipeptidyl-Peptidase 8 (DPP8) (AA 161-245) antibodyDPP8 适用: 人 IHC, ELISA, IHC (p) 宿主: 小鼠 Monoclonal unconjugated
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- 应用备注
- Approved: IHC, IHC-P (5 μg/mL)
- 说明
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Target Species of Antibody: Human
- 实验流程
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The IHC-pro Immunohistochemistry Protocol
Tissue Preparation
Formalin fixation and embedding in paraffin wax
Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.
Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.
Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.
Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.
Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip - 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- Lot specific
- 缓冲液
- PBS, less than 0.1 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
- 有效期
- 12 months
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- 抗原
- DPP8 (Dipeptidyl-Peptidase 8 (DPP8))
- 别名
- Dipeptidylpeptidase 8 / DPP8 (DPP8 产品)
- 背景
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Name/Gene ID: DPP8
Subfamily: Serine S9B
Family: Protease
Synonyms: DPP8, Dipeptidyl-peptidase 8, DPRP1, Dipeptidyl peptidase VIII, Dipeptidylpeptidase 8, DPRP-1, Dipeptidyl peptidase 8, MSTP141, Prolyl dipeptidase DPP8, DP8, DPP VIII - 基因ID
- 54878
- NCBI登录号
- NP_569118
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