Dopamine d2 Receptor 抗体 (Cytoplasmic Domain)
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- 抗原 See all Dopamine d2 Receptor (DRD2) 抗体
- Dopamine d2 Receptor (DRD2) (Dopamine Receptor D2 (DRD2))
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抗原表位
- Cytoplasmic Domain
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适用
- 人, 犬, Bat, Cow, 马, 兔
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This Dopamine d2 Receptor antibody is un-conjugated
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应用范围
- Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 品牌
- IHC-plus™
- 特异性
- Human DRD2. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
- 预测反应
- Percent identity with other species by BLAST analysis: Human, Chimpanzee, Gorilla, Ferret, Bat, Bovine, Dog, Elephant, Panda, Horse, Rabbit (100%) Gibbon, Monkey, Pig (94%) Marmoset (81%).
- 纯化方法
- Immunoaffinity purified
- 免疫原
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Synthetic 16 amino acid peptide from 3rd cytoplasmic domain of human DRD2. Percent identity with other species by BLAST analysis: Human, Chimpanzee, Gorilla, Ferret, Bat, Bovine, Dog, Elephant, Panda, Horse, Rabbit (100%), Gibbon, Monkey, Pig (94%), Marmoset (81%).
Type of Immunogen: Synthetic peptide - Top Product
- Discover our top product DRD2 Primary Antibody
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anti-Dopamine Receptor D2 (DRD2) (AA 194-421) antibody
DRD2 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Dopamine Receptor D2 (DRD2) (AA 201-300) antibodyDRD2 适用: 人, 大鼠, 小鼠 WB, ELISA, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Dopamine Receptor D2 (DRD2) (AA 1-110) antibodyDRD2 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 1B11 unconjugated
anti-Dopamine Receptor D2 (DRD2) (AA 307-336), (C-Term) antibodyDRD2 适用: 人, 小鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-Dopamine Receptor D2 (DRD2) (AA 301-400) antibodyDRD2 适用: 人, 大鼠, 小鼠 WB, ELISA, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Dopamine Receptor D2 (DRD2) (AA 201-300) antibody (AbBy Fluor® 488)DRD2 适用: 人, 大鼠, 小鼠 WB, IF (cc), IF (p) 宿主: 兔 Polyclonal AbBy Fluor® 488
anti-Dopamine Receptor D2 (DRD2) antibodyDRD2 适用: 犬 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Dopamine Receptor D2 (DRD2) (AA 214-373) antibodyDRD2 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Dopamine Receptor D2 (DRD2) (AA 11-26), (Extracellular), (N-Term) antibodyDRD2 适用: 人, 大鼠, 小鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-Dopamine Receptor D2 (DRD2) (AA 11-26), (Extracellular), (N-Term) antibody (FITC)DRD2 适用: 人, 大鼠, 小鼠 FACS 宿主: 兔 Polyclonal FITC
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- 应用备注
- Approved: IHC, IHC-P (10 - 20 μg/mL)
- 说明
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Target Species of Antibody: Human
- 实验流程
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The IHC-pro Immunohistochemistry Protocol
Tissue Preparation
Formalin fixation and embedding in paraffin wax
Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.
Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.
Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.
Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.
Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip - 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- Lot specific
- 缓冲液
- PBS, less than 0.1 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
- 有效期
- 12 months
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- 抗原
- Dopamine d2 Receptor (DRD2) (Dopamine Receptor D2 (DRD2))
- 别名
- DRD2 / Dopamine Receptor D2 (DRD2 产品)
- 别名
- D2DR antibody, D2R antibody, drd2b antibody, d2r2 antibody, Drd-2 antibody, d2dr antibody, d2r antibody, drd2 antibody, drd2-B antibody, DRD2 antibody, D2 antibody, CG18741 antibody, CG7569 antibody, DAMB antibody, DDR2 antibody, DOPR2 antibody, DOPR99B antibody, Dar2 antibody, Dmel\\CG18741 antibody, DopD1 antibody, DopR99B antibody, DopRII antibody, dopamine receptor D2 antibody, dopamine receptor D2 like antibody, dopamine receptor D2 L homeolog antibody, dopamine receptor D2 S homeolog antibody, Dopamine 1-like receptor 2 antibody, DRD2 antibody, drd2l antibody, drd2 antibody, Drd2 antibody, drd2.L antibody, drd2.S antibody, Dop1R2 antibody
- 背景
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Name/Gene ID: DRD2
Subfamily: Dopamine
Family: GPCR
Synonyms: DRD2, D2 dopamine receptor, D2R, Dopamine receptor D2 isoform, Dopamine 2 receptor, D(2) dopamine receptor, D2DR, Dopamine D2 receptor, Dopamine receptor D2 - 基因ID
- 1813
- 途径
- Positive Regulation of Peptide Hormone Secretion, Negative Regulation of Hormone Secretion, cAMP Metabolic Process, Inositol Metabolic Process, Regulation of G-Protein Coupled Receptor Protein Signaling, Feeding Behaviour, Negative Regulation of Transporter Activity, Regulation of long-term Neuronal Synaptic Plasticity
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