DHRS7 抗体 (Internal Region)
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- 抗原 See all DHRS7 抗体
- DHRS7 (Dehydrogenase/reductase (SDR Family) Member 7 (DHRS7))
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抗原表位
- Internal Region
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适用
- 人, 小鼠, 马, Cow, 犬, 兔, Bat, 猴
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This DHRS7 antibody is un-conjugated
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应用范围
- Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 品牌
- IHC-plus™
- 特异性
- Human DHRS7. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
- 预测反应
- Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Mouse, Elephant, Panda, Dog, Bat, Bovine, Horse, Rabbit (100%) Rat, Hamster, Opossum (93%) Marmoset, Pig, Xenopus (87%) Sea anemone (80%).
- 纯化方法
- Immunoaffinity purified
- 免疫原
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Synthetic 15 amino acid peptide from internal region of human DHRS7. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Mouse, Elephant, Panda, Dog, Bat, Bovine, Horse, Rabbit (100%), Rat, Hamster, Opossum (93%), Marmoset, Pig, Xenopus (87%), Sea anemone (80%).
Type of Immunogen: Synthetic peptide - Top Product
- Discover our top product DHRS7 Primary Antibody
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anti-Dehydrogenase/reductase (SDR Family) Member 7 (DHRS7) (AA 29-339) antibody
DHRS7 适用: 人 ELISA, WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Dehydrogenase/reductase (SDR Family) Member 7 (DHRS7) (AA 1-339) antibodyDHRS7 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-Dehydrogenase/reductase (SDR Family) Member 7 (DHRS7) antibodyDHRS7 适用: 人 ELISA, WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Dehydrogenase/reductase (SDR Family) Member 7 (DHRS7) (Internal Region) antibodyDHRS7 适用: 人, 马, Bat IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Dehydrogenase/reductase (SDR Family) Member 7 (DHRS7) (AA 66-93), (N-Term) antibodyDHRS7 适用: 人 WB, FACS, IHC (p) 宿主: 兔 Polyclonal RB25722 unconjugated
anti-Dehydrogenase/reductase (SDR Family) Member 7 (DHRS7) (C-Term) antibodyDHRS7 适用: 人, 大鼠 ELISA, WB 宿主: 兔 Polyclonal unconjugated
anti-Dehydrogenase/reductase (SDR Family) Member 7 (DHRS7) (AA 51-100) antibodyDHRS7 适用: 人, 小鼠, 马, Cow, 犬, 兔, Bat, Pig, 猴 WB 宿主: 兔 Polyclonal unconjugated
anti-Dehydrogenase/reductase (SDR Family) Member 7 (DHRS7) (N-Term) antibodyDHRS7 适用: 人, 小鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-Dehydrogenase/reductase (SDR Family) Member 7 (DHRS7) (C-Term) antibodyDHRS7 适用: 人 IHC (p) 宿主: 兔 Polyclonal unconjugated
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- 应用备注
- Approved: IHC, IHC-P (10 μg/mL)
- 说明
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Target Species of Antibody: Human
- 实验流程
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The IHC-pro Immunohistochemistry Protocol
Tissue Preparation
Formalin fixation and embedding in paraffin wax
Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.
Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.
Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.
Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.
Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip - 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- Lot specific
- 缓冲液
- PBS, less than 0.1 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
- 有效期
- 12 months
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- 抗原
- DHRS7 (Dehydrogenase/reductase (SDR Family) Member 7 (DHRS7))
- 别名
- DHRS7 (DHRS7 产品)
- 别名
- DR-NR3 antibody, MGC80755 antibody, MGC107821 antibody, MGC197379 antibody, MGC197409 antibody, zgc:154087 antibody, SDR34C1 antibody, retDSR4 antibody, retSDR4 antibody, 2310016E22Rik antibody, 5730564L20Rik antibody, AW061210 antibody, Retdsr4 antibody, Retsdr4 antibody, dehydrogenase/reductase (SDR family) member 7-like 1 antibody, dehydrogenase/reductase 7 antibody, dehydrogenase/reductase (SDR family) member 7 L homeolog antibody, dehydrogenase/reductase (SDR family) member 7 antibody, Dhrs7l1 antibody, DHRS7 antibody, dhrs7.L antibody, dhrs7 antibody, Dhrs7 antibody
- 背景
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Name/Gene ID: DHRS7
Synonyms: DHRS7, CGI-86, DHRS7A, RetDSR4, RetSDR4, SDR34C1 - 基因ID
- 51635
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