CXCR7 抗体 (Extracellular Domain)
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- 抗原 See all CXCR7 抗体
- CXCR7 (Chemokine (C-X-C Motif) Receptor 7 (CXCR7))
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抗原表位
- Extracellular Domain
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适用
- 人, 小鼠, 大鼠, 犬, Pig, Cow, 马, Bat, 兔, Hamster
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This CXCR7 antibody is un-conjugated
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应用范围
- Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 品牌
- IHC-plus™
- 特异性
- Human CXCR7. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
- 预测反应
- Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Mouse, Rat, Bovine, Bat, Dog, Hamster, Panda, Horse, Rabbit, Pig (100%) Marmoset, Elephant (95%) Opossum, Platypus (84%).
- 纯化方法
- Immunoaffinity purified
- 免疫原
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Synthetic 19 amino acid peptide from 2nd extracellular domain of human CXCR7. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Mouse, Rat, Bovine, Bat, Dog, Hamster, Panda, Horse, Rabbit, Pig (100%), Marmoset, Elephant (95%), Opossum, Platypus (84%).
Type of Immunogen: Synthetic peptide - Top Product
- Discover our top product CXCR7 Primary Antibody
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anti-Chemokine (C-X-C Motif) Receptor 7 (CXCR7) antibody
KO Validated CXCR7 适用: 人 WB, ELISA, FACS, IHC (p), IF, IHC (fro), IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Chemokine (C-X-C Motif) Receptor 7 (CXCR7) antibodyCXCR7 适用: 人, 小鼠, 大鼠 IHC 宿主: 兔 Polyclonal unconjugated
anti-Chemokine (C-X-C Motif) Receptor 7 (CXCR7) (Internal Region) antibodyCXCR7 适用: 人, 小鼠, 大鼠 WB, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Chemokine (C-X-C Motif) Receptor 7 (CXCR7) (AA 151-250) antibodyCXCR7 适用: 人, 大鼠 WB, ELISA, IHC (p), IF (cc), IF (p), IHC (fro), ICC 宿主: 兔 Polyclonal unconjugated
anti-Chemokine (C-X-C Motif) Receptor 7 (CXCR7) (C-Term) antibodyCXCR7 适用: 人, 小鼠, 大鼠 WB, IHC (p), IF, EIA 宿主: 兔 Polyclonal unconjugated
anti-Chemokine (C-X-C Motif) Receptor 7 (CXCR7) (Cytoplasmic Domain) antibodyCXCR7 适用: 人, 马 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Chemokine (C-X-C Motif) Receptor 7 (CXCR7) antibodyCXCR7 适用: 人 FACS 宿主: 小鼠 Monoclonal 10D1-J16 unconjugated
anti-Chemokine (C-X-C Motif) Receptor 7 (CXCR7) (AA 311-360) antibodyCXCR7 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IHC (p), IF 宿主: 兔 Polyclonal unconjugated
anti-Chemokine (C-X-C Motif) Receptor 7 (CXCR7) (AA 50-150) antibodyCXCR7 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Chemokine (C-X-C Motif) Receptor 7 (CXCR7) (AA 330-358), (C-Term) antibodyCXCR7 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal RB23520 unconjugated
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- 应用备注
- Approved: IHC, IHC-P (10 μg/mL)
- 说明
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Target Species of Antibody: Human
- 实验流程
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The IHC-pro Immunohistochemistry Protocol
Tissue Preparation
Formalin fixation and embedding in paraffin wax
Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.
Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.
Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.
Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.
Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip - 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- Lot specific
- 缓冲液
- PBS, less than 0.1 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
- 有效期
- 12 months
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- 抗原
- CXCR7 (Chemokine (C-X-C Motif) Receptor 7 (CXCR7))
- 别名
- ACKR3 / CXCR7 (CXCR7 产品)
- 别名
- CMKOR1 antibody, CXC-R7 antibody, CXCR-7 antibody, CXCR7 antibody, GPR159 antibody, RDC-1 antibody, RDC1 antibody, AW541270 antibody, Cmkor1 antibody, Cxcr7 antibody, Rdc1 antibody, ackr3 antibody, cxcr7 antibody, rdc1 antibody, xcxcr7 antibody, atypical chemokine receptor 3 antibody, ORF 74; similar to Kaposi's sarcoma-associated herpesvirus ORF 74 antibody, atypical chemokine receptor 3 L homeolog antibody, ACKR3 antibody, MmrVgp88 antibody, Ackr3 antibody, ackr3.L antibody
- 背景
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Name/Gene ID: ACKR3
Subfamily: Chemokine
Family: GPCR
Synonyms: ACKR3, Chemokine orphan receptor 1, CMKOR1, CXCR7, CXC-R7, GPRN1, RDC1, RDC-1, CXCR-7, G-protein coupled receptor 159, GPR159 - 基因ID
- 57007
- 途径
- Myometrial Relaxation and Contraction, Negative Regulation of intrinsic apoptotic Signaling
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