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- 抗原 See all CXCR3 抗体
- CXCR3 (Chemokine (C-X-C Motif) Receptor 3 (CXCR3))
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抗原表位
- C-Term
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适用
- 人, 猴
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This CXCR3 antibody is un-conjugated
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应用范围
- Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 品牌
- IHC-plus™
- 特异性
- Human CXCR3. BLAST analysis of the peptide immunogen showed no homology with other human proteins, except SRRM2 (59 % ).
- 预测反应
- Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset (100%) Goat, Panda (82%).
- 纯化方法
- Immunoaffinity purified
- 免疫原
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Synthetic 17 amino acid peptide from C-terminus of human CXCR3. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset (100%), Goat, Panda (82%).
Type of Immunogen: Synthetic peptide - Top Product
- Discover our top product CXCR3 Primary Antibody
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anti-Chemokine (C-X-C Motif) Receptor 3 (CXCR3) antibody
CXCR3 适用: 人 FACS, WB, ELISA, IHC 宿主: 小鼠 Monoclonal 5C10E6 unconjugated
anti-Chemokine (C-X-C Motif) Receptor 3 (CXCR3) (AA 301-367) antibodyCXCR3 适用: 人, 小鼠, 大鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-Chemokine (C-X-C Motif) Receptor 3 (CXCR3) (C-Term) antibodyVerified CXCR3 适用: 人 FACS, ELISA, IHC, IF 宿主: 山羊 Polyclonal unconjugated
anti-Chemokine (C-X-C Motif) Receptor 3 (CXCR3) (AA 1-368) antibodyCXCR3 适用: 人, 大鼠 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Chemokine (C-X-C Motif) Receptor 3 (CXCR3) (AA 4-50) antibodyCXCR3 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Chemokine (C-X-C Motif) Receptor 3 (CXCR3) (AA 1-100) antibodyCXCR3 适用: 人, 小鼠 FACS, WB, ELISA, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Chemokine (C-X-C Motif) Receptor 3 (CXCR3) (Internal Region) antibodyCXCR3 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Chemokine (C-X-C Motif) Receptor 3 (CXCR3) (AA 140-167) antibodyCXCR3 适用: 人 FACS, WB, IHC (p) 宿主: 兔 Polyclonal RB23384 unconjugated
anti-Chemokine (C-X-C Motif) Receptor 3 (CXCR3) (AA 147-175), (Middle Region) antibodyCXCR3 适用: 人 FACS, WB, IHC (p), EIA 宿主: 兔 Polyclonal unconjugated
anti-Chemokine (C-X-C Motif) Receptor 3 (CXCR3) antibody (FITC)GMP Grade CXCR3 适用: 人 FACS 宿主: 小鼠 Monoclonal 1C6 FITC
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- 应用备注
- Approved: IHC, IHC-P (10 - 20 μg/mL)
- 说明
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Target Species of Antibody: Human
- 实验流程
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The IHC-pro Immunohistochemistry Protocol
Tissue Preparation
Formalin fixation and embedding in paraffin wax
Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.
Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.
Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.
Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.
Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip - 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- Lot specific
- 缓冲液
- PBS, less than 0.1 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
- 有效期
- 12 months
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- 抗原
- CXCR3 (Chemokine (C-X-C Motif) Receptor 3 (CXCR3))
- 别名
- CXCR3 (CXCR3 产品)
- 别名
- CD182 antibody, CD183 antibody, CKR-L2 antibody, CMKAR3 antibody, GPR9 antibody, IP10-R antibody, Mig-R antibody, MigR antibody, Cd183 antibody, Cmkar3 antibody, CXCR3 antibody, cxcr3 antibody, Gpr9 antibody, CXC-R3 antibody, CXCR-3 antibody, zgc:92301 antibody, sb:eu378 antibody, C-X-C motif chemokine receptor 3 antibody, chemokine (C-X-C motif) receptor 3 antibody, chemokine (C-X-C motif) receptor 3, tandem duplicate 2 antibody, chemokine (C-X-C motif) receptor 3, tandem duplicate 1 antibody, CXCR3 antibody, Cxcr3 antibody, cxcr3 antibody, cxcr3.2 antibody, cxcr3.1 antibody
- 背景
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Name/Gene ID: CXCR3
Subfamily: Chemokine
Family: GPCR
Synonyms: CXCR3, C-x-c chemokine receptor 3, CD183 antigen, CKR-L2, CXCR-3, CMKAR3, CD182, CD183, Cxcr3a, GPR9, IP-10 receptor, IP10-R, Mig receptor, MigR, Mig-R, Chemokine (C-X-C) receptor 3, CXC-R3, Cxcr3b, G protein-coupled receptor 9, IP10 receptor - 基因ID
- 2833
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