CHRM5 抗体 (Cytoplasmic Domain)
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- 抗原 See all CHRM5 抗体
- CHRM5 (Cholinergic Receptor, Muscarinic 5 (CHRM5))
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抗原表位
- Cytoplasmic Domain
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适用
- 人, 猴
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This CHRM5 antibody is un-conjugated
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应用范围
- Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 品牌
- IHC-plus™
- 特异性
- Human CHRM5. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
- 预测反应
- Percent identity with other species by BLAST analysis: Human, Gorilla, Monkey (100%) Gibbon (94%) Chimpanzee, Marmoset (88%) Dog, Bovine, Panda, Horse (81%).
- 纯化方法
- Immunoaffinity purified
- 免疫原
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Synthetic 16 amino acid peptide from 3rd cytoplasmic domain of human CHRM5. Percent identity with other species by BLAST analysis: Human, Gorilla, Monkey (100%), Gibbon (94%), Chimpanzee, Marmoset (88%), Dog, Bovine, Panda, Horse (81%).
Type of Immunogen: Synthetic peptide - Top Product
- Discover our top product CHRM5 Primary Antibody
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anti-Cholinergic Receptor, Muscarinic 5 (CHRM5) (C-Term) antibody
CHRM5 适用: 人, 小鼠, 大鼠 ELISA, WB, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Cholinergic Receptor, Muscarinic 5 (CHRM5) antibodyCHRM5 适用: 人 ELISA, FACS 宿主: 小鼠 Monoclonal 7E5H11 unconjugated
anti-Cholinergic Receptor, Muscarinic 5 (CHRM5) (AA 220-427) antibodyCHRM5 适用: 人 ELISA, WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Cholinergic Receptor, Muscarinic 5 (CHRM5) antibodyCHRM5 适用: 人, 小鼠, 大鼠 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Cholinergic Receptor, Muscarinic 5 (CHRM5) (AA 1-532) antibodyCHRM5 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-Cholinergic Receptor, Muscarinic 5 (CHRM5) (AA 230-430) antibodyCHRM5 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Cholinergic Receptor, Muscarinic 5 (CHRM5) antibodyCHRM5 适用: 人, 小鼠 WB, IF, IC 宿主: 兔 Polyclonal unconjugated
anti-Cholinergic Receptor, Muscarinic 5 (CHRM5) (AA 281-330) antibodyCHRM5 适用: 人 ELISA, WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Cholinergic Receptor, Muscarinic 5 (CHRM5) (AA 7-24), (Extracellular), (N-Term) antibodyCHRM5 适用: 人, 大鼠 WB 宿主: 兔 Polyclonal unconjugated
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- 应用备注
- Approved: IHC, IHC-P (6 μg/mL)
- 说明
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Target Species of Antibody: Human
- 实验流程
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The IHC-pro Immunohistochemistry Protocol
Tissue Preparation
Formalin fixation and embedding in paraffin wax
Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.
Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.
Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.
Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.
Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip - 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- Lot specific
- 缓冲液
- PBS, less than 0.1 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
- 有效期
- 12 months
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- 抗原
- CHRM5 (Cholinergic Receptor, Muscarinic 5 (CHRM5))
- 别名
- CHRM5 / M5 (CHRM5 产品)
- 别名
- HM5 antibody, chrm5 antibody, wu:fd11g11 antibody, si:ch211-150c22.1 antibody, AChR-M5 antibody, M5R antibody, m5 antibody, CHKM5MR antibody, zgc:194865 antibody, zgc:194868 antibody, cholinergic receptor muscarinic 5 antibody, cholinergic receptor, muscarinic 5 antibody, cholinergic receptor, muscarinic 5b antibody, cholinergic receptor, muscarinic 5a antibody, CHRM5 antibody, Chrm5 antibody, chrm5b antibody, chrm5a antibody
- 背景
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Name/Gene ID: CHRM5
Subfamily: Acetylcholine
Family: GPCR
Synonyms: CHRM5, M5 muscarinic receptor, Muscarinic m5 receptor, Muscarinic receptor M5, HM5 - 基因ID
- 1133
- 途径
- Synaptic Membrane
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