-
- 抗原 See all CD97 抗体
- CD97
-
抗原表位
- N-Term
-
适用
- 人
-
宿主
- 兔
-
克隆类型
- 多克隆
-
标记
- This CD97 antibody is un-conjugated
-
应用范围
- Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 品牌
- IHC-plus™
- 特异性
- Human CD97. BLAST analysis of the peptide immunogen showed no homology with other human proteins, except MAP3K12 (58 % ).
- 预测反应
- Percent identity with other species by BLAST analysis: Human (100%) Monkey (95%).
- 纯化方法
- Immunoaffinity purified
- 免疫原
-
Synthetic 19 amino acid peptide from N-terminal extracellular domain of human CD97. Percent identity with other species by BLAST analysis: Human (100%), Monkey (95%).
Type of Immunogen: Synthetic peptide - Top Product
- Discover our top product CD97 Primary Antibody
-
anti-CD97 (CD97) (AA 419-552) antibody
CD97 适用: 人 WB, IHC, ELISA, FACS, ICC, Neut 宿主: 小鼠 Monoclonal 6D7E6 unconjugated
anti-CD97 (CD97) (AA 741-835) antibodyCD97 适用: 人 ELISA, FACS, IF (p), IF (cc), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-CD97 (CD97) (AA 421-529) antibodyCD97 适用: 人 WB, ELISA, RNAi 宿主: 小鼠 Monoclonal 5D5 unconjugated
anti-CD97 (CD97) (AA 58-260) antibodyCD97 适用: 人 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal C3 unconjugated
anti-CD97 (CD97) (AA 240-435) antibodyCD97 适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-CD97 (CD97) antibodyCD97 适用: 人 IHC, ELISA, IF 宿主: 兔 Monoclonal 3A7 unconjugated Recombinant Antibody
anti-CD97 (CD97) antibody (FITC)CD97 适用: 人 FACS 宿主: 小鼠 Monoclonal MEM-180 FITC
anti-CD97 (CD97) (AA 32-173) antibodyCD97 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-CD97 (CD97) (AA 421-529) antibodyCD97 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
anti-CD97 (CD97) (AA 1-742) antibodyCD97 适用: 人 WB, FACS 宿主: 小鼠 Polyclonal unconjugated
-
- 应用备注
- Approved: ICC, IHC, IHC-P (5 μg/mL)
- 说明
-
Target Species of Antibody: Human
- 实验流程
-
The IHC-pro Immunohistochemistry Protocol
Tissue Preparation
Formalin fixation and embedding in paraffin wax
Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.
Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.
Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.
Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.
Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip - 限制
- 仅限研究用
-
- 状态
- Liquid
- 浓度
- Lot specific
- 缓冲液
- PBS, less than 0.1 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
- 有效期
- 12 months
-
- 抗原
- CD97
- 别名
- ADGRE5 / CD97 (CD97 产品)
- 别名
- AA409984 antibody, TM7LN1 antibody, adhesion G protein-coupled receptor E5 antibody, Adgre5 antibody, ADGRE5 antibody
- 背景
-
Name/Gene ID: ADGRE5
Subfamily: CD55
Family: GPCR
Synonyms: ADGRE5, CD97, CD97 Molecule, Leukocyte antigen CD97, TM7LN1, CD97 antigen - 基因ID
- 976
-
You are here:
