CCKBR 抗体 (Extracellular Domain)
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- 抗原 See all CCKBR 抗体
- CCKBR (Cholecystokinin B Receptor (CCKBR))
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抗原表位
- Extracellular Domain
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适用
- 人
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This CCKBR antibody is un-conjugated
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应用范围
- Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 品牌
- IHC-plus™
- 特异性
- Human CCKBR. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
- 预测反应
- Percent identity with other species by BLAST analysis: Human (100%) Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Hamster, Elephant, Bat, Bovine, Dog, Horse, Rabbit (95%) Rat (90%) Panda (85%).
- 纯化方法
- Immunoaffinity purified
- 免疫原
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Synthetic 20 amino acid peptide from 3rd extracellular domain of human CCKBR. Percent identity with other species by BLAST analysis: Human (100%), Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Hamster, Elephant, Bat, Bovine, Dog, Horse, Rabbit (95%), Rat (90%), Panda (85%).
Type of Immunogen: Synthetic peptide - Top Product
- Discover our top product CCKBR Primary Antibody
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anti-Cholecystokinin B Receptor (CCKBR) (Internal Region) antibody
Verified CCKBR 适用: 人, 小鼠 WB, ELISA, IHC 宿主: 山羊 Polyclonal unconjugated
anti-Cholecystokinin B Receptor (CCKBR) (AA 301-400) antibodyCCKBR 适用: 大鼠, 小鼠 WB, ELISA, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Cholecystokinin B Receptor (CCKBR) (AA 243-333) antibodyCCKBR 适用: 人 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Cholecystokinin B Receptor (CCKBR) (N-Term) antibodyCCKBR 适用: 人 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Cholecystokinin B Receptor (CCKBR) (C-Term) antibodyCCKBR 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Cholecystokinin B Receptor (CCKBR) (Cytoplasmic Domain) antibodyCCKBR 适用: 人 IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Cholecystokinin B Receptor (CCKBR) antibodyCCKBR 适用: 人, 大鼠, 小鼠 WB, FACS, IHC (fro), ICC 宿主: 兔 Polyclonal unconjugated
anti-Cholecystokinin B Receptor (CCKBR) (AA 39-53), (Extracellular), (N-Term) antibodyCCKBR 适用: 人, 大鼠, 小鼠 WB, IHC, FACS 宿主: 兔 Polyclonal unconjugated
anti-Cholecystokinin B Receptor (CCKBR) (N-Term) antibodyCCKBR 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Cholecystokinin B Receptor (CCKBR) (N-Term) antibodyCCKBR 适用: 人, 大鼠, 小鼠, 猴 WB, ELISA 宿主: 兔 Polyclonal unconjugated
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- 应用备注
- Approved: IHC, IHC-P (11 μg/mL)
- 说明
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Target Species of Antibody: Human
- 实验流程
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The IHC-pro Immunohistochemistry Protocol
Tissue Preparation
Formalin fixation and embedding in paraffin wax
Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.
Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.
Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.
Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.
Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip - 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- Lot specific
- 缓冲液
- PBS, less than 0.1 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
- 有效期
- 12 months
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- 抗原
- CCKBR (Cholecystokinin B Receptor (CCKBR))
- 别名
- CCKBR / Cckb (CCKBR 产品)
- 别名
- CCKBR antibody, LOC100232835 antibody, CCK-B antibody, CCK2R antibody, GASR antibody, Cck2r antibody, Cholrec antibody, CCKR-2 antibody, CCK-BR antibody, CCK2-R antibody, CCK-CHR antibody, cholecystokinin B receptor antibody, cholecystokinin receptor-like antibody, CCKBR antibody, LOC100232835 antibody, Cckbr antibody
- 背景
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Name/Gene ID: CCKBR
Subfamily: Cholecystokinin
Family: GPCR
Synonyms: CCKBR, CCKB, Cholecystokinin-2 receptor, CCK-BR, CCK2 receptor, CCK2-R, CCKB-R, Cholecystokinin-B, GASR, CCK-B, CCK-B receptor, CCK2R, Cckb receptor, CCKRB, Cholecystokinin B receptor, Gastrin receptor - 基因ID
- 887
- 途径
- Positive Regulation of Peptide Hormone Secretion, Feeding Behaviour
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