CALCRL 抗体 (Cytoplasmic Domain)
(1 reference)-
- 抗原 See all CALCRL 抗体
- CALCRL (Calcitonin Receptor-Like (CALCRL))
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抗原表位
- Cytoplasmic Domain
- 适用
- 人, 大鼠, Cow, 犬, 马, 兔, 山羊, 猴, Hamster, Pig, 非洲爪蟾
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This CALCRL antibody is un-conjugated
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应用范围
- Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 品牌
- IHC-plus™
- 特异性
- Human CALCRL. BLAST analysis of the peptide immunogen showed no homology with other human proteins, except CALCR (56 % ).
- 预测反应
- Percent identity with other species by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Rat, Goat, Hamster, Panda, Dog, Bovine, Horse, Rabbit, Pig, Opossum, Xenopus (100%) Mouse, Elephant, Bat, Guinea pig, Stickleback, Pufferfish, Zebrafish (94%) Turkey, Chicken (81%).
- 纯化方法
- Immunoaffinity purified
- 免疫原
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Synthetic 16 amino acid peptide from 3rd cytoplasmic domain of human CALCRL. Percent identity with other species by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Rat, Goat, Hamster, Panda, Dog, Bovine, Horse, Rabbit, Pig, Opossum, Xenopus (100%), Mouse, Elephant, Bat, Guinea pig, Stickleback, Pufferfish, Zebrafish (94%), Turkey, Chicken (81%).
Type of Immunogen: Synthetic peptide - Top Product
- Discover our top product CALCRL Primary Antibody
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anti-Calcitonin Receptor-Like (CALCRL) (AA 522-536), (C-Term), (Intracellular) antibody
CALCRL 适用: 人, 小鼠, 大鼠 WB, IHC, FACS 宿主: 兔 Polyclonal unconjugated
anti-Calcitonin Receptor-Like (CALCRL) antibodyCALCRL 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Calcitonin Receptor-Like (CALCRL) (AA 23-132) antibodyCALCRL 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
anti-Calcitonin Receptor-Like (CALCRL) (AA 340-367) antibodyCALCRL 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal RB22070 unconjugated
anti-Calcitonin Receptor-Like (CALCRL) antibodyCALCRL 适用: 人, 小鼠, 大鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-Calcitonin Receptor-Like (CALCRL) (C-Term) antibodyCALCRL 适用: 人, 小鼠, 大鼠 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Calcitonin Receptor-Like (CALCRL) (AA 108-157) antibodyCALCRL 适用: 人, 小鼠, 大鼠, Cow, 犬, 马, 兔, 豚鼠, 猴 WB 宿主: 兔 Polyclonal unconjugated
anti-Calcitonin Receptor-Like (CALCRL) (AA 392-461) antibodyCALCRL 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Calcitonin Receptor-Like (CALCRL) (N-Term) antibodyCALCRL 适用: 人, 小鼠, 大鼠 WB 宿主: 兔 Polyclonal unconjugated
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- 应用备注
- Approved: IHC, IHC-P (34 μg/mL)
- 说明
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Target Species of Antibody: Human
- 实验流程
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The IHC-pro Immunohistochemistry Protocol
Tissue Preparation
Formalin fixation and embedding in paraffin wax
Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.
Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.
Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.
Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.
Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip - 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- Lot specific
- 缓冲液
- PBS, less than 0.1 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
- 有效期
- 12 months
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: "Excitatory Effects of Calcitonin Gene-Related Peptide (CGRP) on Superficial Sp5C Neurons in Mouse Medullary Slices." in: International journal of molecular sciences, Vol. 22, Issue 7, (2021) (PubMed).
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: "Excitatory Effects of Calcitonin Gene-Related Peptide (CGRP) on Superficial Sp5C Neurons in Mouse Medullary Slices." in: International journal of molecular sciences, Vol. 22, Issue 7, (2021) (PubMed).
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- 抗原
- CALCRL (Calcitonin Receptor-Like (CALCRL))
- 别名
- CALCRL / CGRP Receptor (CALCRL 产品)
- 别名
- CALCRL antibody, CGRPR antibody, CRLR antibody, AV071593 antibody, CLR antibody, Crlr antibody, RATCRLR antibody, RNCLR antibody, calcitonin receptor like receptor antibody, calcitonin receptor-like antibody, calcitonin receptor like receptor L homeolog antibody, CALCRL antibody, LOC100168906 antibody, calcrl antibody, Calcrl antibody, calcrl.L antibody
- 背景
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Name/Gene ID: CALCRL
Subfamily: Adrenomedullin
Family: GPCR
Synonyms: CALCRL, CGRP type 1 receptor, Cgrp1 receptor, CRLR, Calcitonin receptor-like, CGRP receptor, CGRPR - 基因ID
- 10203
- 途径
- cAMP Metabolic Process
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