Cell Adhesion Molecule 4 抗体 (Internal Region)
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- 抗原 See all Cell Adhesion Molecule 4 (CADM4) 抗体
- Cell Adhesion Molecule 4 (CADM4)
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抗原表位
- Internal Region
- 适用
- 人, 小鼠, 大鼠, 犬, Cow, 马, 兔, 猴, Bat, Pig
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This Cell Adhesion Molecule 4 antibody is un-conjugated
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应用范围
- Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 品牌
- IHC-plus™
- 特异性
- Human CADM4 / NECL-4. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
- 预测反应
- Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Elephant, Panda, Bovine, Dog, Bat, Horse, Rabbit, Pig, Opossum, Platypus (100%).
- 纯化方法
- Immunoaffinity purified
- 免疫原
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Synthetic 14 amino acid peptide from internal region of human CADM4 / NECL-4. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Elephant, Panda, Bovine, Dog, Bat, Horse, Rabbit, Pig, Opossum, Platypus (100%).
Type of Immunogen: Synthetic peptide
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anti-Cell Adhesion Molecule 4 (CADM4) (C-Term) antibody
CADM4 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Cell Adhesion Molecule 4 (CADM4) (C-Term) antibodyVerified CADM4 适用: 人 WB, ELISA, IHC 宿主: 山羊 Polyclonal unconjugated
anti-Cell Adhesion Molecule 4 (CADM4) (AA 339-388) antibodyCADM4 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Cell Adhesion Molecule 4 (CADM4) (C-Term) antibodyCADM4 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Cell Adhesion Molecule 4 (CADM4) (C-Term) antibodyCADM4 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Cell Adhesion Molecule 4 (CADM4) (Internal Region) antibodyVerified CADM4 适用: 人 WB, ELISA, IHC 宿主: 山羊 Polyclonal unconjugated
anti-Cell Adhesion Molecule 4 (CADM4) (AA 376-388) antibodyCADM4 适用: 小鼠, 大鼠 WB, ICC 宿主: 兔 Polyclonal unconjugated
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- 应用备注
- Approved: IHC, IHC-P (10 - 15 μg/mL)
- 说明
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Target Species of Antibody: Human
- 实验流程
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The IHC-pro Immunohistochemistry Protocol
Tissue Preparation
Formalin fixation and embedding in paraffin wax
Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.
Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.
Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.
Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.
Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip - 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- Lot specific
- 缓冲液
- PBS, less than 0.1 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
- 有效期
- 12 months
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- 抗原
- Cell Adhesion Molecule 4 (CADM4)
- 别名
- IGSF4C / CADM4 (CADM4 产品)
- 别名
- CADM4 antibody, IGSF4C antibody, zgc:101704 antibody, NECL4 antibody, Necl-4 antibody, TSLL2 antibody, synCAM4 antibody, Igdf4c antibody, Igsf4c antibody, Tsll2 antibody, Upar antibody, cell adhesion molecule 4 antibody, cell adhesion molecule 4 L homeolog antibody, CADM4 antibody, cadm4 antibody, Cadm4 antibody, cadm4.L antibody
- 背景
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Name/Gene ID: CADM4
Synonyms: CADM4, Cell adhesion molecule 4, Nectin-like 4, Nectin-like protein 4, Necl-4, NECL4, IGSF4C, TSLC1-like 2, SynCAM4, TSLC1-like protein 2, TSLL2 - 基因ID
- 199731
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