Bestrophin 4 抗体 (Internal Region)
-
- 抗原 See all Bestrophin 4 (BEST4) 抗体
- Bestrophin 4 (BEST4)
-
抗原表位
- Internal Region
-
适用
- 人, 猴
-
宿主
- 兔
-
克隆类型
- 多克隆
-
标记
- This Bestrophin 4 antibody is un-conjugated
-
应用范围
- Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC)
- 品牌
- IHC-plus™
- 特异性
- Human BEST4. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
- 预测反应
- Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset (100%) Rabbit (93%) Hamster (87%) Pig (80%).
- 纯化方法
- Immunoaffinity purified
- 免疫原
-
Synthetic 15 amino acid peptide from internal region of human BEST4. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset (100%), Rabbit (93%), Hamster (87%), Pig (80%).
Type of Immunogen: Synthetic peptide
-
anti-Bestrophin 4 (BEST4) (Internal Region) antibody
BEST4 适用: 人, Cow, 兔, Pig, Bat IHC (p), IHC 宿主: 兔 Polyclonal unconjugated
anti-Bestrophin 4 (BEST4) (N-Term) antibodyBEST4 适用: 人, 犬, Cow, 兔, 豚鼠, 小鼠, Bat, 猴 WB 宿主: 兔 Polyclonal unconjugated
anti-Bestrophin 4 (BEST4) (N-Term) antibodyBEST4 适用: 人, 犬 WB 宿主: 兔 Polyclonal unconjugated
anti-Bestrophin 4 (BEST4) (N-Term) antibodyBEST4 适用: 人, 犬, Cow, 兔, 豚鼠, 小鼠, 马, 大鼠, 斑马鱼 WB 宿主: 兔 Polyclonal unconjugated
-
- 应用备注
- Approved: IHC, IHC-P (2.5 μg/mL)
- 说明
-
Target Species of Antibody: Human
- 实验流程
-
The IHC-pro Immunohistochemistry Protocol
Tissue Preparation
Formalin fixation and embedding in paraffin wax
Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.
Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.
Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.
Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.
Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip - 限制
- 仅限研究用
-
- 状态
- Liquid
- 浓度
- Lot specific
- 缓冲液
- PBS, less than 0.1 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
- 有效期
- 12 months
-
- 抗原
- Bestrophin 4 (BEST4)
- 别名
- BEST4 (BEST4 产品)
- 别名
- CG7259 antibody, CT22395 antibody, Dmel\\CG7259 antibody, dbest4 antibody, BEST4 antibody, VMD2L2 antibody, vmd2l2 antibody, Bestrophin 4 antibody, bestrophin 4 antibody, Best4 antibody, BEST4 antibody, best4 antibody
- 背景
-
Name/Gene ID: BEST4
Subfamily: Bestrophin
Family: Ion channel
Synonyms: BEST4, Bestrophin-4, Bestrophin 4, VMD2L2 - 基因ID
- 266675
-
