Attractin 抗体 (Internal Region)
-
- 抗原 See all Attractin (ATRN) 抗体
- Attractin (ATRN)
-
抗原表位
- Internal Region
- 适用
- 人, 小鼠, 大鼠, Bat, 犬, Hamster, Pig, Cow, 马, 猴, 兔, 斑马鱼
-
宿主
- 兔
-
克隆类型
- 多克隆
-
标记
- This Attractin antibody is un-conjugated
-
应用范围
- Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 品牌
- IHC-plus™
- 特异性
- Human ATRN / Attractin. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
- 预测反应
- Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bat, Bovine, Horse, Rabbit, Pig, Zebrafish (100%) Opossum, Turkey, Chicken, Platypus, Xenopus (94%) Stickleback (88%).
- 纯化方法
- Immunoaffinity purified
- 免疫原
-
Synthetic 17 amino acid peptide from internal region of human ATRN / Attractin. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bat, Bovine, Horse, Rabbit, Pig, Zebrafish (100%), Opossum, Turkey, Chicken, Platypus, Xenopus (94%), Stickleback (88%).
Type of Immunogen: Synthetic peptide - Top Product
- Discover our top product ATRN Primary Antibody
-
anti-Attractin (ATRN) (AA 751-850) antibody
ATRN 适用: 人, 小鼠 ELISA, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Attractin (ATRN) (AA 84-350) antibodyATRN 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Attractin (ATRN) antibodyATRN 适用: 人, 小鼠, 大鼠 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Attractin (ATRN) (N-Term) antibodyATRN 适用: 人 ELISA, IHC, WB 宿主: 兔 Polyclonal unconjugated
anti-Attractin (ATRN) (AA 32-66), (N-Term) antibodyATRN 适用: 人 WB 宿主: 兔 Polyclonal RB48734 unconjugated
-
- 应用备注
- Approved: IHC, IHC-P (10 μg/mL)
- 说明
-
Target Species of Antibody: Human
- 实验流程
-
The IHC-pro Immunohistochemistry Protocol
Tissue Preparation
Formalin fixation and embedding in paraffin wax
Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.
Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.
Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.
Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.
Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip - 限制
- 仅限研究用
-
- 状态
- Liquid
- 浓度
- Lot specific
- 缓冲液
- PBS, less than 0.1 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
- 有效期
- 12 months
-
- 抗原
- Attractin (ATRN)
- 别名
- ATRN / Attractin (ATRN 产品)
- 别名
- DPPT-L antibody, MGCA antibody, AW558010 antibody, Mgca antibody, mKIAA0548 antibody, mahogany antibody, mg antibody, attractin antibody, ATRN antibody, Atrn antibody, ATT antibody
- 背景
-
Name/Gene ID: ATRN
Synonyms: ATRN, Attractin-2, Attractin, DPPT-L, KIAA0548, Mahogany protein, MGCA, Mahogany homolog - 基因ID
- 8455
-
