Apelin Receptor 抗体 (Cytoplasmic Domain)
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- 抗原 See all Apelin Receptor (APLNR) 抗体
- Apelin Receptor (APLNR)
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抗原表位
- Cytoplasmic Domain
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适用
- 人, 马, Hamster
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This Apelin Receptor antibody is un-conjugated
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应用范围
- Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 品牌
- IHC-plus™
- 特异性
- Human Apelin Receptor. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
- 预测反应
- Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Hamster, Elephant, Panda, Horse (100%) Orangutan, Marmoset, Mouse, Rat, Bat, Rabbit (94%).
- 纯化方法
- Immunoaffinity purified
- 免疫原
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Synthetic 16 amino acid peptide from 1st cytoplasmic domain of human Apelin Receptor. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Hamster, Elephant, Panda, Horse (100%), Orangutan, Marmoset, Mouse, Rat, Bat, Rabbit (94%).
Type of Immunogen: Synthetic peptide - Top Product
- Discover our top product APLNR Primary Antibody
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anti-Apelin Receptor (APLNR) (AA 52-66) antibody
APLNR 适用: 人, 大鼠, 小鼠 WB, IF (p), IHC (p), FACS 宿主: 兔 Polyclonal unconjugated
anti-Apelin Receptor (APLNR) (AA 309-380) antibodyAPLNR 适用: 人 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Apelin Receptor (APLNR) (C-Term) antibodyAPLNR 适用: 人, 小鼠, 兔, Hamster, 猴 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Apelin Receptor (APLNR) (Extracellular Domain) antibodyAPLNR 适用: 人, 猴 IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Apelin Receptor (APLNR) antibodyAPLNR 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-Apelin Receptor (APLNR) (AA 310-380) antibodyAPLNR 适用: 人 WB, ELISA, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Apelin Receptor (APLNR) (Cytoplasmic Domain) antibodyAPLNR 适用: 人, 小鼠 IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Apelin Receptor (APLNR) (AA 46-95) antibodyAPLNR 适用: 人, 马, Hamster, 豚鼠, Bat WB 宿主: 兔 Polyclonal unconjugated
anti-Apelin Receptor (APLNR) (N-Term) antibodyAPLNR 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Apelin Receptor (APLNR) (AA 141-190) antibodyAPLNR 适用: 人 WB, ELISA, IF 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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Approved: IHC, IHC-P (4 - 7 μg/mL)
Not recommended for: WB - 说明
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Target Species of Antibody: Human
- 实验流程
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The IHC-pro Immunohistochemistry Protocol
Tissue Preparation
Formalin fixation and embedding in paraffin wax
Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.
Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.
Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.
Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.
Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip - 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- Lot specific
- 缓冲液
- PBS, less than 0.1 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
- 有效期
- 12 months
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- 抗原
- Apelin Receptor (APLNR)
- 别名
- APLNR/ Apelin Receptor / APJ (APLNR 产品)
- 别名
- AGTRL1 antibody, APJ antibody, APJR antibody, HG11 antibody, Agtrl1 antibody, msr/apj antibody, Apj antibody, X-msr antibody, Xangio1 antibody, Xmsr antibody, agtrl1 antibody, angio1 antibody, apj antibody, apjr antibody, hg11 antibody, msr antibody, APLNR antibody, DKFZp469C1532 antibody, agtrl1-B antibody, agtrl1b antibody, Agtrl1a antibody, grn antibody, zgc:114063 antibody, APJa antibody, XAngio1 antibody, agtrl1-A antibody, agtrl1a antibody, aplnr-a antibody, aplnr-b antibody, x-MSR antibody, xAngio1 antibody, APLNR1 antibody, apelin receptor antibody, apelin receptor S homeolog antibody, apelin receptor b antibody, apelin receptor L homeolog antibody, APLNR antibody, Aplnr antibody, aplnr antibody, aplnr.S antibody, aplnrb antibody, aplnr.L antibody
- 背景
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Name/Gene ID: APLNR
Subfamily: Apelin
Family: GPCR
Synonyms: APLNR, AGTRL1, Angiotensin receptor-like 1, Angiotensin II receptor-like 1, Apelin receptor, APJ, APJ (apelin) receptor, APJR, G protein-coupled receptor APJ, HG11 orphan receptor, HG11, APJ receptor, G-protein coupled receptor APJ - 基因ID
- 187
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