ADRA1D 抗体 (Extracellular Domain)
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- 抗原 See all ADRA1D 抗体
- ADRA1D (Adrenergic Receptor, alpha 1d (ADRA1D))
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抗原表位
- Extracellular Domain
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适用
- 人, 犬, 马, 豚鼠, 猴, Pig
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This ADRA1D antibody is un-conjugated
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应用范围
- Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 品牌
- IHC-plus™
- 特异性
- Human ADRA1D. BLAST analysis of the peptide immunogen showed no homology with other human proteins, except ADRA1B (56 % ).
- 预测反应
- Percent identity with other species by BLAST analysis: Human, Gorilla, Monkey, Marmoset, Dog, Panda, Horse, Pig, Guinea pig (100%) Mouse, Rat, Bovine, Hamster, Rabbit, Opossum (94%) Bat (89%).
- 纯化方法
- Immunoaffinity purified
- 免疫原
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Synthetic 18 amino acid peptide from 2nd extracellular domain of human ADRA1D. Percent identity with other species by BLAST analysis: Human, Gorilla, Monkey, Marmoset, Dog, Panda, Horse, Pig, Guinea pig (100%), Mouse, Rat, Bovine, Hamster, Rabbit, Opossum (94%), Bat (89%).
Type of Immunogen: Synthetic peptide - Top Product
- Discover our top product ADRA1D Primary Antibody
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anti-Adrenergic Receptor, alpha 1d (ADRA1D) (AA 1-30), (N-Term) antibody
ADRA1D 适用: 人, 大鼠 WB, IHC (p), FACS 宿主: 兔 Polyclonal RB46798 unconjugated
anti-Adrenergic Receptor, alpha 1d (ADRA1D) (3rd Extracellular Loop), (AA 231-245), (Cys240) antibodyADRA1D 适用: 人, 大鼠, 小鼠 IHC, WB, IF, FACS, ICC, LCI 宿主: 兔 Polyclonal unconjugated
anti-Adrenergic Receptor, alpha 1d (ADRA1D) (AA 286-335) antibodyADRA1D 适用: 人, 大鼠, 小鼠, 犬, 马, Cow, 豚鼠, 兔, 猴, Pig, Bat WB 宿主: 兔 Polyclonal unconjugated
anti-Adrenergic Receptor, alpha 1d (ADRA1D) (C-Term) antibodyADRA1D 适用: 人, 大鼠, 小鼠 IHC, WB, IF, IC 宿主: 兔 Polyclonal unconjugated
anti-Adrenergic Receptor, alpha 1d (ADRA1D) (N-Term) antibodyADRA1D 适用: 人, 大鼠 WB, IHC (p), FACS 宿主: 兔 Polyclonal unconjugated
anti-Adrenergic Receptor, alpha 1d (ADRA1D) (C-Term) antibodyADRA1D 适用: 人 IHC, WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Adrenergic Receptor, alpha 1d (ADRA1D) (Cytoplasmic Domain) antibodyADRA1D 适用: 人, 犬, 马, Cow, 兔, 猴, Pig, Bat IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Adrenergic Receptor, alpha 1d (ADRA1D) antibodyADRA1D 适用: 人, 大鼠, 小鼠 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Adrenergic Receptor, alpha 1d (ADRA1D) (C-Term) antibodyADRA1D 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Adrenergic Receptor, alpha 1d (ADRA1D) (AA 451-500) antibodyADRA1D 适用: 人 IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
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- 应用备注
- Approved: IHC, IHC-P (2.5 μg/mL)
- 说明
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Target Species of Antibody: Human
- 实验流程
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The IHC-pro Immunohistochemistry Protocol
Tissue Preparation
Formalin fixation and embedding in paraffin wax
Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.
Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.
Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.
Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.
Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip - 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- Lot specific
- 缓冲液
- PBS, less than 0.1 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
- 有效期
- 12 months
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- 抗原
- ADRA1D (Adrenergic Receptor, alpha 1d (ADRA1D))
- 别名
- ADRA1D (ADRA1D 产品)
- 别名
- Adra-1 antibody, Adra1 antibody, Adra1a antibody, Gpcr8 antibody, Spr8 antibody, [a]1d antibody, alpha1D-AR antibody, Adrd1 antibody, ADRA1 antibody, ADRA1A antibody, ADRA1R antibody, ALPHA1 antibody, DAR antibody, dJ779E11.2 antibody, adrenergic receptor, alpha 1d antibody, adrenoceptor alpha 1D antibody, Adra1d antibody, ADRA1D antibody
- 背景
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Name/Gene ID: ADRA1D
Subfamily: Adrenergic
Family: GPCR
Synonyms: ADRA1D, Alpha 1d, Alpha-1D adrenergic receptor, ADRA1R, Alpha-1D adrenoceptor, Alpha-1D adrenoreceptor, ALPHA1, Alpha-1D-adrenergic receptor, Alpha-adrenergic receptor 1a, Alpha1delta-AR, DAR, ADRA1, Adrenoceptor alpha 1D, Alpha-1A adrenergic receptor, DJ779E11.2 - 基因ID
- 146
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