ADRA1B 抗体 (C-Term)
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- 抗原 See all ADRA1B 抗体
- ADRA1B (Adrenergic, alpha-1B-, Receptor (ADRA1B))
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抗原表位
- C-Term
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适用
- 人, 小鼠, 大鼠, 犬, Cow, Hamster, 猴
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This ADRA1B antibody is un-conjugated
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应用范围
- Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 品牌
- IHC-plus™
- 特异性
- Human ADRA1B. BLAST analysis of the peptide immunogen showed no homology with other human proteins, except PLEKHG5 (45 % ).
- 预测反应
- Percent identity with other species by BLAST analysis: Human, Gorilla, Monkey, Marmoset, Mouse, Rat, Hamster, Bovine, Dog (100%) Elephant (90%) Bat (85%) Opossum, Platypus (80%).
- 纯化方法
- Immunoaffinity purified
- 免疫原
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Synthetic 20 amino acid peptide from C-terminus of human ADRA1B. Percent identity with other species by BLAST analysis: Human, Gorilla, Monkey, Marmoset, Mouse, Rat, Hamster, Bovine, Dog (100%), Elephant (90%), Bat (85%), Opossum, Platypus (80%).
Type of Immunogen: Synthetic peptide - Top Product
- Discover our top product ADRA1B Primary Antibody
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anti-Adrenergic, alpha-1B-, Receptor (ADRA1B) antibody
ADRA1B 适用: 人, 小鼠, 大鼠 IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Adrenergic, alpha-1B-, Receptor (ADRA1B) antibodyADRA1B 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Adrenergic, alpha-1B-, Receptor (ADRA1B) (AA 21-35), (Extracellular), (N-Term) antibodyADRA1B 适用: 人, 小鼠, 大鼠 WB, IHC, IF, FACS, ICC, LCI 宿主: 兔 Polyclonal unconjugated
anti-Adrenergic, alpha-1B-, Receptor (ADRA1B) (Cytoplasmic Domain) antibodyADRA1B 适用: 人, 小鼠, 大鼠, Pig, 犬, Cow, Hamster, 马, 猴 ELISA, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Adrenergic, alpha-1B-, Receptor (ADRA1B) (AA 169-218) antibodyADRA1B 适用: 人, 小鼠, 大鼠, Pig, 犬, 豚鼠, 兔, Hamster, 马, 猴, Bat WB 宿主: 兔 Polyclonal unconjugated
anti-Adrenergic, alpha-1B-, Receptor (ADRA1B) (AA 388-437) antibodyADRA1B 适用: 人, 小鼠, 大鼠, Pig, 犬, Cow, 豚鼠, 兔, Hamster, 猴 WB 宿主: 兔 Polyclonal unconjugated
anti-Adrenergic, alpha-1B-, Receptor (ADRA1B) (AA 1-100) antibodyADRA1B 适用: 小鼠 WB, ELISA, IHC (p), IF (cc), IF (p), ICC, IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Adrenergic, alpha-1B-, Receptor (ADRA1B) (C-Term) antibodyADRA1B 适用: 人 WB, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Adrenergic, alpha-1B-, Receptor (ADRA1B) antibodyADRA1B 适用: 人, 小鼠, 大鼠 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Adrenergic, alpha-1B-, Receptor (ADRA1B) (Cytoplasmic Domain) antibodyADRA1B 适用: 人, 猴 IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
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- 应用备注
- Approved: IHC, IHC-P (11 μg/mL)
- 说明
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Target Species of Antibody: Human
- 实验流程
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The IHC-pro Immunohistochemistry Protocol
Tissue Preparation
Formalin fixation and embedding in paraffin wax
Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.
Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.
Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.
Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.
Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip - 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- Lot specific
- 缓冲液
- PBS, less than 0.1 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
- 有效期
- 12 months
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- 抗原
- ADRA1B (Adrenergic, alpha-1B-, Receptor (ADRA1B))
- 别名
- ADRA1B (ADRA1B 产品)
- 别名
- LOC100136712 antibody, [a]1b antibody, ADRA1 antibody, ALPHA1BAR antibody, RDC5 antibody, alpha-1B adrenoceptor antibody, adrenoceptor alpha 1B antibody, adrenergic receptor, alpha 1b antibody, LOC100136712 antibody, ADRA1B antibody, Adra1b antibody
- 背景
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Name/Gene ID: ADRA1B
Subfamily: Adrenergic
Family: GPCR
Synonyms: ADRA1B, Alpha 1b, Alpha-1B adrenergic receptor, Alpha-1b-adrenergic, Alpha-1B-adrenergic receptor, Alpha1b, Adrenoceptor alpha 1B, Alpha-1B adrenoreceptor, Alpha1B-AR, Alpha1beta-AR, ALPHA1BAR, ADRA1, Alpha-1B adrenoceptor, Alpha1b-adrenergic - 基因ID
- 147
- 途径
- AMPK Signaling, Carbohydrate Homeostasis, Regulation of Carbohydrate Metabolic Process
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