alpha 1 Adrenergic Receptor 抗体 (Cytoplasmic Domain)
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- 抗原 See all alpha 1 Adrenergic Receptor (ADRA1A) 抗体
- alpha 1 Adrenergic Receptor (ADRA1A) (Adrenoceptor alpha 1A (ADRA1A))
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抗原表位
- Cytoplasmic Domain
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适用
- 人, 大鼠, 小鼠, Cow, 犬, 兔, 马, 绵羊, 猴, Hamster, Pig
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This alpha 1 Adrenergic Receptor antibody is un-conjugated
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应用范围
- Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)
- 品牌
- IHC-plus™
- 特异性
- Human ADRA1A. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
- 预测反应
- Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Sheep, Bovine, Dog, Hamster, Elephant, Panda, Horse, Rabbit, Pig (100%) Bat, Guinea pig (94%) Opossum, Platypus (88%).
- 纯化方法
- Immunoaffinity purified
- 免疫原
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Synthetic 16 amino acid peptide from 3rd cytoplasmic domain of human ADRA1A. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Sheep, Bovine, Dog, Hamster, Elephant, Panda, Horse, Rabbit, Pig (100%), Bat, Guinea pig (94%), Opossum, Platypus (88%).
Type of Immunogen: Synthetic peptide - Top Product
- Discover our top product ADRA1A Primary Antibody
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anti-Adrenoceptor alpha 1A (ADRA1A) (AA 346-475) antibody
ADRA1A 适用: 人 WB, IF, IHC 宿主: 兔 Polyclonal unconjugated
anti-Adrenoceptor alpha 1A (ADRA1A) antibodyADRA1A 适用: 人 WB, IF, IHC (p), ICC 宿主: 兔 Polyclonal unconjugated
anti-Adrenoceptor alpha 1A (ADRA1A) (Internal Region) antibodyADRA1A 适用: 人, 大鼠, 小鼠 WB, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Adrenoceptor alpha 1A (ADRA1A) (AA 206-270) antibodyADRA1A 适用: 人, 大鼠, 小鼠, Cow, 兔, 猴 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Adrenoceptor alpha 1A (ADRA1A) (AA 6-22) antibodyADRA1A 适用: 人 WB, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Adrenoceptor alpha 1A (ADRA1A) (AA 1-27) antibodyADRA1A 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 5G8 unconjugated
anti-Adrenoceptor alpha 1A (ADRA1A) (AA 330-466) antibodyADRA1A 适用: 人 WB, IHC, ICC 宿主: 兔 Polyclonal unconjugated
anti-Adrenoceptor alpha 1A (ADRA1A) (C-Term) antibodyADRA1A 适用: 人, 大鼠, 小鼠 WB, IF, IHC, IC 宿主: 兔 Polyclonal unconjugated
anti-Adrenoceptor alpha 1A (ADRA1A) (2nd Extracellular Loop), (AA 171-183), (Cys176) antibodyADRA1A 适用: 人, 大鼠, 小鼠 WB, IF, IHC 宿主: 兔 Polyclonal unconjugated
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- 应用备注
- Approved: ICC, IHC, IHC-P (3 μg/mL)
- 说明
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Target Species of Antibody: Human
- 实验流程
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The IHC-pro Immunohistochemistry Protocol
Tissue Preparation
Formalin fixation and embedding in paraffin wax
Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.
Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.
Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.
Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.
Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip - 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- Lot specific
- 缓冲液
- PBS, less than 0.1 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
- 有效期
- 12 months
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- 抗原
- alpha 1 Adrenergic Receptor (ADRA1A) (Adrenoceptor alpha 1A (ADRA1A))
- 别名
- ADRA1A (ADRA1A 产品)
- 别名
- ADRA1C antibody, ADRA1L1 antibody, ALPHA1AAR antibody, Adra1c antibody, ALPHA-1A antibody, alpha-1A antibody, adrenoceptor alpha 1A antibody, adrenergic receptor, alpha 1a antibody, ADRA1A antibody, Adra1a antibody
- 背景
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Name/Gene ID: ADRA1A
Subfamily: Adrenergic
Family: GPCR
Synonyms: ADRA1A, ADRA1L1, Adrenergic alpha 1c receptor, Alpha 1c adrenoceptor, Alpha-adrenergic receptor 1c, ADRA1C, Adrenoceptor alpha 1A, Alpha-1A adrenoceptor, Alpha-1A adrenoreceptor, Alpha-1A adrenergic receptor, Alpha-1C adrenergic receptor, ALPHA1AAR, Alpha1C-AR - 基因ID
- 148
- 途径
- AMPK Signaling
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