-
- 抗原 See all ADORA1 抗体
- ADORA1 (Adenosine A1 Receptor (ADORA1))
-
抗原表位
- C-Term
-
适用
- 人, 大鼠
-
宿主
- 兔
-
克隆类型
- 多克隆
-
标记
- This ADORA1 antibody is un-conjugated
-
应用范围
- Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 品牌
- IHC-plus™
- 特异性
- Human Adenosine A1 Receptor. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
- 预测反应
- Percent identity with other species by BLAST analysis: Human, Gibbon, Monkey (100%) Orangutan, Marmoset, Elephant, Horse (95%) Rat, Pig, Guinea pig (90%) Bovine, Hamster, Panda (85%) Mouse, Dog (80%).
- 纯化方法
- Immunoaffinity purified
- 免疫原
-
Synthetic 20 amino acid peptide from C-terminus of human Adenosine A1 Receptor. Percent identity with other species by BLAST analysis: Human, Gibbon, Monkey (100%), Orangutan, Marmoset, Elephant, Horse (95%), Rat, Pig, Guinea pig (90%), Bovine, Hamster, Panda (85%), Mouse, Dog (80%).
Type of Immunogen: Synthetic peptide - Top Product
- Discover our top product ADORA1 Primary Antibody
-
anti-Adenosine A1 Receptor (ADORA1) antibody
ADORA1 适用: 人 WB, IHC 宿主: 兔 Monoclonal unconjugated
anti-Adenosine A1 Receptor (ADORA1) (AA 225-326) antibodyADORA1 适用: 人, 小鼠 WB, ELISA, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Adenosine A1 Receptor (ADORA1) (C-Term) antibody (Atto 390)ADORA1 适用: 人 WB, IHC, IF, ICC 宿主: 兔 Polyclonal Atto 390
anti-Adenosine A1 Receptor (ADORA1) (C-Term) antibodyADORA1 适用: 人 WB, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Adenosine A1 Receptor (ADORA1) (C-Term) antibody (Alkaline Phosphatase (AP))ADORA1 适用: 人 WB, IHC, IF, ICC 宿主: 兔 Polyclonal Alkaline Phosphatase (AP)
anti-Adenosine A1 Receptor (ADORA1) (C-Term) antibody (APC)ADORA1 适用: 人 WB, IHC, IF, ICC 宿主: 兔 Polyclonal APC
anti-Adenosine A1 Receptor (ADORA1) (3rd Intracellular Loop), (AA 213-229) antibodyADORA1 适用: 人, 大鼠, 小鼠 WB, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Adenosine A1 Receptor (ADORA1) antibodyADORA1 适用: 人 WB, ELISA, IHC 宿主: 兔 Monoclonal 10D12 unconjugated Recombinant Antibody
anti-Adenosine A1 Receptor (ADORA1) (C-Term) antibodyADORA1 适用: 人, 大鼠, Pig WB 宿主: 兔 Polyclonal unconjugated
anti-Adenosine A1 Receptor (ADORA1) (C-Term) antibodyVerified ADORA1 适用: 人 WB, ELISA, FACS 宿主: 山羊 Polyclonal unconjugated
-
- 应用备注
- Approved: IHC, IHC-P (7.2 μg/mL)
- 说明
-
Target Species of Antibody: Human
- 实验流程
-
The IHC-pro Immunohistochemistry Protocol
Tissue Preparation
Formalin fixation and embedding in paraffin wax
Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.
Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.
Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.
Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.
Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip - 限制
- 仅限研究用
-
- 状态
- Liquid
- 浓度
- Lot specific
- 缓冲液
- PBS, less than 0.1 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
- 有效期
- 12 months
-
- 抗原
- ADORA1 (Adenosine A1 Receptor (ADORA1))
- 别名
- ADORA1 / Adenosine A1 Receptor (ADORA1 产品)
- 别名
- adora1-A antibody, a1R antibody, ADORA1 antibody, LOC606743 antibody, LOC100222054 antibody, RDC7 antibody, A1-AR antibody, A1AR antibody, A1R antibody, AA1R antibody, AI848715 antibody, BB176431 antibody, Ri antibody, adenosine A1 receptor L homeolog antibody, adenosine A1 receptor antibody, adenosine receptor A1 antibody, adora1.L antibody, ADORA1 antibody, adora1 antibody, LOC100222054 antibody, Adora1 antibody
- 背景
-
Name/Gene ID: ADORA1
Subfamily: Adenosine
Family: GPCR
Synonyms: ADORA1, A1 adenosine receptor, A1ar, Adenosine A1 receptor, Adenosine receptor A1, ADO-A1 receptor, RDC7 - 基因ID
- 134
- 途径
- EGFR Signaling Pathway, Negative Regulation of Hormone Secretion, Synaptic Membrane
-
You are here:
