HTR2C 抗体 (Cytoplasmic Domain)
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- 抗原 See all HTR2C 抗体
- HTR2C (5-Hydroxytryptamine (serotonin) Receptor 2C (HTR2C))
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抗原表位
- Cytoplasmic Domain
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适用
- 人
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This HTR2C antibody is un-conjugated
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应用范围
- Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 品牌
- IHC-plus™
- 特异性
- Human 5HT2C Receptor. BLAST analysis of the peptide immunogen showed no homology with other human proteins, except ANKRD55 (50 % ).
- 预测反应
- Percent identity with other species by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Marmoset (100%) Monkey (95%) Elephant, Panda, Horse (80%).
- 纯化方法
- Immunoaffinity purified
- 免疫原
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Synthetic 20 amino acid peptide from 3rd cytoplasmic domain of human 5HT2C Receptor. Percent identity with other species by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Marmoset (100%), Monkey (95%), Elephant, Panda, Horse (80%).
Type of Immunogen: Synthetic peptide - Top Product
- Discover our top product HTR2C Primary Antibody
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anti-5-Hydroxytryptamine (serotonin) Receptor 2C (HTR2C) (N-Term) antibody
HTR2C 适用: 人, 小鼠, 大鼠, Pig, 马, 兔 WB 宿主: 兔 Polyclonal unconjugated
anti-5-Hydroxytryptamine (serotonin) Receptor 2C (HTR2C) (Internal Region) antibodyHTR2C 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-5-Hydroxytryptamine (serotonin) Receptor 2C (HTR2C) (AA 387-446) antibodyHTR2C 适用: 人 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-5-Hydroxytryptamine (serotonin) Receptor 2C (HTR2C) antibodyHTR2C 适用: 人 WB, ELISA, IHC 宿主: 兔 Monoclonal 9H8 unconjugated Recombinant Antibody
anti-5-Hydroxytryptamine (serotonin) Receptor 2C (HTR2C) (AA 1-52) antibodyHTR2C 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 1A8 unconjugated
anti-5-Hydroxytryptamine (serotonin) Receptor 2C (HTR2C) (C-Term) antibodyHTR2C 适用: 人 WB, IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-5-Hydroxytryptamine (serotonin) Receptor 2C (HTR2C) (AA 311-410) antibodyHTR2C 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-5-Hydroxytryptamine (serotonin) Receptor 2C (HTR2C) (AA 274-308) antibodyHTR2C 适用: 人 WB 宿主: 兔 Polyclonal RB52766 unconjugated
anti-5-Hydroxytryptamine (serotonin) Receptor 2C (HTR2C) (AA 1-52) antibodyHTR2C 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
anti-5-Hydroxytryptamine (serotonin) Receptor 2C (HTR2C) (AA 28-77) antibodyHTR2C 适用: 人, 小鼠, 大鼠, 犬, Pig, 马, 兔, 猴 WB 宿主: 兔 Polyclonal unconjugated
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- 应用备注
- Approved: IHC, IHC-P (5.6 μg/mL)
- 说明
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Target Species of Antibody: Human
- 实验流程
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The IHC-pro Immunohistochemistry Protocol
Tissue Preparation
Formalin fixation and embedding in paraffin wax
Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.
Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.
Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.
Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.
Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip - 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- Lot specific
- 缓冲液
- PBS, less than 0.1 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
- 有效期
- 12 months
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- 抗原
- HTR2C (5-Hydroxytryptamine (serotonin) Receptor 2C (HTR2C))
- 别名
- HTR2C / 5-HT2C Receptor (HTR2C 产品)
- 别名
- 5-HT2C antibody, 5-HTR2C antibody, HTR1C antibody, 5-HT2cR antibody, 5HT1c antibody, Htr1c antibody, SR1 antibody, 5HT-1C antibody, 5HT2C antibody, 5-HT-2C antibody, 5-ht2cr antibody, si:dkey-24g18.2 antibody, 5ht2c antibody, 5HTR2C antibody, 5-hydroxytryptamine receptor 2C antibody, 5-hydroxytryptamine (serotonin) receptor 2C antibody, 5-hydroxytryptamine (serotonin) receptor 2C, G protein-coupled S homeolog antibody, 5-hydroxytryptamine (serotonin) receptor 2C, G protein-coupled-like 1 antibody, 5-hydroxytryptamine (serotonin) receptor 2C, G protein-coupled antibody, HTR2C antibody, Htr2c antibody, htr2c.S antibody, htr2cl1 antibody, LOC407134 antibody
- 背景
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Name/Gene ID: HTR2C
Subfamily: Serotonin
Family: GPCR
Synonyms: HTR2C, 5-HT-1C, 5-HT1c receptor, 5HT-1C, 5-HT2C, 5-HT2c receptor, 5HT2C Receptor, 5-HT-2C, 5-HT1C, 5-HTR2C, HTR1C, Serotonin 5-HT-1c receptor, Serotonin receptor 2C, Serotonin 1c receptor, Serotonin 5-HT-2C receptor, Serotonin 2c receptor - 基因ID
- 3358
- 途径
- Inositol Metabolic Process, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour
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