HTR1F 抗体 (C-Term)
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- 抗原 See all HTR1F 抗体
- HTR1F (5-Hydroxytryptamine (serotonin) Receptor 1F (HTR1F))
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抗原表位
- C-Term
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适用
- 人, 大鼠, 小鼠, 猴, Cow, 兔, 犬, Bat, 豚鼠, 小鸡, Hamster
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This HTR1F antibody is un-conjugated
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应用范围
- Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC)
- 品牌
- IHC-plus™
- 特异性
- Human 5HT1F Receptor. BLAST analysis of the peptide immunogen showed no homology with other human proteins, except HTR1E (71 % ), HTR1D (59 %), HTR1B (59 %).
- 预测反应
- Percent identity with other species by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bovine, Bat, Rabbit, Guinea pig, Turkey, Chicken, Platypus (100%) Xenopus, Stickleback, Pufferfish (94%) Opossum, Lizard (88%) Horse (82%).
- 纯化方法
- Immunoaffinity purified
- 免疫原
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Synthetic 17 amino acid peptide from C-terminus of human 5HT1F Receptor. Percent identity with other species by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bovine, Bat, Rabbit, Guinea pig, Turkey, Chicken, Platypus (100%), Xenopus, Stickleback, Pufferfish (94%), Opossum, Lizard (88%), Horse (82%).
Type of Immunogen: Synthetic peptide - Top Product
- Discover our top product HTR1F Primary Antibody
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anti-5-Hydroxytryptamine (serotonin) Receptor 1F (HTR1F) (AA 1-100) antibody
HTR1F 适用: 人, 大鼠, 小鼠 WB, ELISA, IHC (p), IF (cc), IF (p), ICC, IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-5-Hydroxytryptamine (serotonin) Receptor 1F (HTR1F) (AA 213-248) antibodyHTR1F 适用: 人 WB 宿主: 兔 Polyclonal RB52189 unconjugated
anti-5-Hydroxytryptamine (serotonin) Receptor 1F (HTR1F) (AA 203-279) antibodyHTR1F 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
anti-5-Hydroxytryptamine (serotonin) Receptor 1F (HTR1F) (N-Term) antibodyHTR1F 适用: 人, 大鼠, 猴, Cow, 兔 IHC (p), IHC 宿主: 兔 Polyclonal unconjugated
anti-5-Hydroxytryptamine (serotonin) Receptor 1F (HTR1F) (Cytoplasmic Domain) antibodyHTR1F 适用: 人, 猴, Bat IHC (p), IHC 宿主: 兔 Polyclonal unconjugated
anti-5-Hydroxytryptamine (serotonin) Receptor 1F (HTR1F) (C-Term) antibodyHTR1F 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-5-Hydroxytryptamine (serotonin) Receptor 1F (HTR1F) (Internal Region) antibodyHTR1F 适用: 人, 大鼠, 小鼠, 猴 WB, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-5-Hydroxytryptamine (serotonin) Receptor 1F (HTR1F) (N-Term) antibodyHTR1F 适用: 人, 大鼠, 猴, Cow, Pig, 兔, Bat, 豚鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-5-Hydroxytryptamine (serotonin) Receptor 1F (HTR1F) (AA 200-290) antibodyHTR1F 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-5-Hydroxytryptamine (serotonin) Receptor 1F (HTR1F) (N-Term) antibodyHTR1F 适用: 人, 大鼠, 小鼠, Pig WB 宿主: 兔 Polyclonal unconjugated
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- 应用备注
- Approved: IHC, IHC-P (2 - 3 μg/mL)
- 说明
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Target Species of Antibody: Human
- 实验流程
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The IHC-pro Immunohistochemistry Protocol
Tissue Preparation
Formalin fixation and embedding in paraffin wax
Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.
Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.
Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.
Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.
Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip - 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- Lot specific
- 缓冲液
- PBS, less than 0.1 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
- 有效期
- 12 months
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- 抗原
- HTR1F (5-Hydroxytryptamine (serotonin) Receptor 1F (HTR1F))
- 别名
- HTR1F / 5-HT1F Receptor (HTR1F 产品)
- 别名
- 5-HT-1F antibody, 5-HT1F antibody, 5HT6 antibody, HTR1EL antibody, MR77 antibody, Htr1eb antibody, 5htr1e antibody, 5htr1f antibody, 5-hydroxytryptamine receptor 1F antibody, 5-hydroxytryptamine (serotonin) receptor 1F antibody, uncharacterized LOC100406194 antibody, HTR1F antibody, Htr1f antibody, LOC100406194 antibody
- 背景
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Name/Gene ID: HTR1F
Subfamily: Serotonin
Family: GPCR
Synonyms: HTR1F, 5-HT1F, 5-HT1f receptor, 5HT1F Receptor, 5-HT-1F, 5HT6, HTR1EL, MR77, Serotonin 1f receptor, Serotonin 5-HT-1f receptor, Serotonin receptor 1F - 基因ID
- 3355
- 途径
- JAK/STAT Signaling
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