Serotonin Receptor 1E 抗体 (Cytoplasmic Domain)
-
- 抗原 See all Serotonin Receptor 1E (HTR1E) 抗体
- Serotonin Receptor 1E (HTR1E)
-
抗原表位
- Cytoplasmic Domain
-
适用
- 人, 马, Cow, 豚鼠, 猴, Bat
-
宿主
- 兔
-
克隆类型
- 多克隆
-
标记
- This Serotonin Receptor 1E antibody is un-conjugated
-
应用范围
- Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC)
- 品牌
- IHC-plus™
- 特异性
- Human 5HT1E Receptor. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
- 预测反应
- Percent identity with other species by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Elephant, Bovine, Bat, Horse, Guinea pig (100%) Panda, Opossum, Turkey, Sparrow, Chicken (94%) Lizard (88%).
- 纯化方法
- Immunoaffinity purified
- 免疫原
-
Synthetic 16 amino acid peptide from 3rd cytoplasmic domain of human 5HT1E Receptor. Percent identity with other species by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Elephant, Bovine, Bat, Horse, Guinea pig (100%), Panda, Opossum, Turkey, Sparrow, Chicken (94%), Lizard (88%).
Type of Immunogen: Synthetic peptide - Top Product
- Discover our top product HTR1E Primary Antibody
-
anti-Serotonin Receptor 1E (HTR1E) (AA 203-291) antibody
HTR1E 适用: 人 ELISA, WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Serotonin Receptor 1E (HTR1E) (Cytoplasmic Domain) antibodyHTR1E 适用: 人, 马, Cow, 豚鼠, 猴, 小鸡 IHC (p), IHC 宿主: 兔 Polyclonal unconjugated
anti-Serotonin Receptor 1E (HTR1E) (AA 223-258) antibodyHTR1E 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal RB53325 unconjugated
anti-Serotonin Receptor 1E (HTR1E) (AA 206-276) antibodyHTR1E 适用: 人 ELISA, WB 宿主: 小鼠 Monoclonal 2E9 unconjugated
anti-Serotonin Receptor 1E (HTR1E) (Cytoplasmic Domain) antibodyHTR1E 适用: 人, 马, 豚鼠, 猴, Bat ELISA, IHC (p), IHC 宿主: 兔 Polyclonal unconjugated
anti-Serotonin Receptor 1E (HTR1E) (3rd Cytoplasmic Domain) antibodyHTR1E 适用: 人 IHC (p) 宿主: 兔 Polyclonal unconjugated
-
- 应用备注
- Approved: IHC, IHC-P (16 - 24 μg/mL)
- 说明
-
Target Species of Antibody: Human
- 实验流程
-
The IHC-pro Immunohistochemistry Protocol
Tissue Preparation
Formalin fixation and embedding in paraffin wax
Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.
Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.
Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.
Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.
Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip - 限制
- 仅限研究用
-
- 状态
- Liquid
- 浓度
- Lot specific
- 缓冲液
- PBS, less than 0.1 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
- 有效期
- 12 months
-
- 抗原
- Serotonin Receptor 1E (HTR1E)
- 别名
- HTR1E / 5-HT1E Receptor (HTR1E 产品)
- 别名
- Htr1ea antibody, HTR1E antibody, 5HT1E antibody, 5-HT1E antibody, 5-HT-1E antibody, 5-hydroxytryptamine (serotonin) receptor 1E antibody, 5-hydroxytryptamine receptor 1E antibody, 5-hydroxytryptamine (serotonin) receptor 1E, G protein-coupled antibody, Htr1e antibody, HTR1E antibody, htr1e antibody
- 背景
-
Name/Gene ID: HTR1E
Subfamily: Serotonin
Family: GPCR
Synonyms: HTR1E, 5-HT1E, 5-HT1e receptor, 5-HT-1E, 5HT1E Receptor, HT1e receptor, S31, Serotonin 5-HT-1e receptor, Serotonin 1e receptor, Serotonin receptor 1E - 基因ID
- 3354
- 途径
- JAK/STAT Signaling, cAMP Metabolic Process
-
