5HT1D 抗体 (Cytoplasmic Domain)
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- 抗原 See all 5HT1D (HTR1D) 抗体
- 5HT1D (HTR1D) (Serotonin Receptor 1D (HTR1D))
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抗原表位
- Cytoplasmic Domain
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适用
- 人, Pig
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This 5HT1D antibody is un-conjugated
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应用范围
- Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 品牌
- IHC-plus™
- 特异性
- Human 5HT1D Receptor. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
- 预测反应
- Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Pig (100%) Marmoset, Bat, Dog, Bovine, Horse, Rabbit, Guinea pig (94%) Mouse, Rat, Panda (88%) Hamster, Elephant, Opossum, Turkey, Chicken (81%).
- 纯化方法
- Immunoaffinity purified
- 免疫原
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Synthetic 16 amino acid peptide from 3rd cytoplasmic domain of human 5HT1D Receptor. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Pig (100%), Marmoset, Bat, Dog, Bovine, Horse, Rabbit, Guinea pig (94%), Mouse, Rat, Panda (88%), Hamster, Elephant, Opossum, Turkey, Chicken (81%).
Type of Immunogen: Synthetic peptide - Top Product
- Discover our top product HTR1D Primary Antibody
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anti-Serotonin Receptor 1D (HTR1D) antibody
HTR1D 适用: 小鼠, 人, 大鼠 WB, ELISA, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Serotonin Receptor 1D (HTR1D) (Internal Region) antibodyHTR1D 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Serotonin Receptor 1D (HTR1D) (Cytoplasmic Domain) antibodyHTR1D 适用: 人, 猴 IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Serotonin Receptor 1D (HTR1D) (AA 150-250) antibodyHTR1D 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
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- 应用备注
- Approved: IHC, IHC-P (5 - 10 μg/mL)
- 说明
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Target Species of Antibody: Human
- 实验流程
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The IHC-pro Immunohistochemistry Protocol
Tissue Preparation
Formalin fixation and embedding in paraffin wax
Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.
Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.
Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.
Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.
Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip - 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- Lot specific
- 缓冲液
- PBS, less than 0.1 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
- 有效期
- 12 months
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- 抗原
- 5HT1D (HTR1D) (Serotonin Receptor 1D (HTR1D))
- 别名
- HTR1D / 5-HT1D Receptor (HTR1D 产品)
- 别名
- AI853647 antibody, Gpcr14 antibody, Htr1db antibody, 5-HT1D antibody, HT1DA antibody, HTR1DA antibody, HTRL antibody, RDC4 antibody, 5HT1D antibody, 5-HT-1D antibody, 5-hydroxytryptamine (serotonin) receptor 1D antibody, 5-hydroxytryptamine receptor 1D antibody, Htr1d antibody, HTR1D antibody
- 背景
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Name/Gene ID: HTR1D
Subfamily: Serotonin
Family: GPCR
Synonyms: HTR1D, 5-HT1d alpha receptor, 5HT1D Receptor, 5-HT1d-type serotonin receptor, 5-HT1D, 5-HT-1D, 5-HT-1D-alpha, 5-HT1d receptor, HT1d receptor, HTR1DA, HTRL, HT1DA, Serotonin receptor 1D, RDC4, Gpcr14, Serotonin 1d receptor, Serotonin 5-HT-1d receptor - 基因ID
- 3352
- 途径
- JAK/STAT Signaling
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