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Nestin 抗体 (AA 1484-1500)

Name: Nestin 抗体 (AA 1484-1500)
Brand: antibodies-online
SKU: ABIN104564
Availability: OutOfStock

NES 适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated

Western blot using Affinity Purified anti-Nestin antibody shows detection of a band ~220 kDa corresponding to mouse Nestin (arrowhead). (Nestin 抗体 (AA 1484-1500))

Immunohistochemistry with anti-nestin antibody showing nestin staining in cytoplasm of of ductal epithelium of human salivary gland (B) and in nucleus and cytoplasm of human breast tissue (C). (Nestin 抗体 (AA 1484-1500))

IHC

ELISA

Western blot using Affinity Purified anti-Nestin antibody shows detection of a band ~220 kDa corresponding to human Nestin (arrowhead). (Nestin 抗体 (AA 1484-1500))

Immunohistochemistry with Anti-nestin antibody Tissue: mouse kidney (Left) and human kidney (Right). (Nestin 抗体 (AA 1484-1500))

IHC

5 images

(5 references)

产品编号 ABIN104564

发货至: 中国

Datasheet as PDF

抗原 See all Nestin (NES) 抗体

Nestin (NES)
抗原表位
16

16

15

10

8

7

7

5

4

4

3

3

2

2

2

2

1

1

1

1

1

1

1

1

1
AA 1484-1500
适用
136

73

52

4

3

2

2

2

1

1

1

1
人
宿主
108

36

3

1

1
兔
Compatible Secondaries
克隆类型
113

36
多克隆
标记
61

10

9

7

7

4

3

3

3

3

3

3

3

3

3

3

3

3

3

3

3

3

3
This Nestin antibody is un-conjugated
应用范围
73

48

47

47

41

39

35

27

25

16

9

6

2

2

1

1
Western Blotting (WB), Immunohistochemistry (IHC), ELISA

产品特性

Concentration Definition: by UV absorbance at 280 nm

免疫原

This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a recombinant protein corresponding to amino acids 1484-1500 of human Nestin protein.
Immunogentype:Recombinant

亚型

IgG

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应用备注

Affinity purified Anti-Nestin has been tested for use in ELISA, Immunohistochemistry (IHC) and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 200-220 kDa in size corresponding to Nestin protein by western blotting in the appropriate cell lysate or extract.

限制

仅限研究用
状态

Liquid

浓度

1.0 mg/mL

缓冲液

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

储存液

Sodium azide

注意事项

This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

-20 °C
Mokrý, Cízková, Filip, Ehrmann, Osterreicher, Kolár, English: "Nestin expression by newly formed human blood vessels." in: Stem cells and development, Vol. 13, Issue 6, pp. 658-64, (2005) (PubMed).

Aihara, Sugawara, Torii, Hosaka, Kurihara, Saito, Takeuchi: "Angiogenic endothelium-specific nestin expression is enhanced by the first intron of the nestin gene." in: Laboratory investigation; a journal of technical methods and pathology, Vol. 84, Issue 12, pp. 1581-92, (2005) (PubMed).

Yaworsky, Kappen: "Heterogeneity of neural progenitor cells revealed by enhancers in the nestin gene." in: Developmental biology, Vol. 205, Issue 2, pp. 309-21, (1999) (PubMed).

Dahlstrand, Zimmerman, McKay, Lendahl: "Characterization of the human nestin gene reveals a close evolutionary relationship to neurofilaments." in: Journal of cell science, Vol. 103 ( Pt 2), Issue 1, pp. 589-97, (1993) (PubMed).

Hockfield, McKay: "Identification of major cell classes in the developing mammalian nervous system." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 5, Issue 12, pp. 3310-28, (1986) (PubMed).
抗原

Nestin (NES)

别名

Nestin (NES 产品)

别名

paranemin antibody, NES antibody, AA166324 antibody, C78523 antibody, ESTM46 antibody, nestin antibody, nestin S homeolog antibody, NES antibody, nes.S antibody, Nes antibody, nes antibody

背景

Nestin, is a large intermediate filament protein (class Type VI) that was first identified with a monoclonal antibody by Hockfield and McKay (1985). Nestin is expressed predominantly in stem cells of the central nervous system in the neural tube. Nestin is expressed during development and in myotendinous and neuromuscular junctions. Nestin expression is restricted, typically disappearing by E18. Nestin is thought to be a reasonable neuronal marker; however, recent studies have found nestin expression in other cell types such as endothelial cells. Nestin expression is seen in almost all GBMs (Glioblastoma mulitformes) and many melanomas (both primary and metastatic) but not in any metastatic carcinomas. Upon terminal neural differentiation, nestin is downregulated and replaced by neurofilaments.
Synonyms: nestin, ESTM 46 antibody, FLJ 21841 antibody, FLJ21841 antibody, Intermediate filament protein antibody

基因ID

10763, 38176300

UniProt

P48681