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Nestin 抗体 (AA 1484-1500)

Rockland 600-401-417 NES 适用: 人, 小鼠 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
Rockland
产品编号 ABIN104564
Supplier Product No.: 600-401-417
发货至: 中国
  • 抗原 See all Nestin (NES) 抗体
    Nestin (NES)
    抗原表位
    • 16
    • 16
    • 15
    • 7
    • 6
    • 5
    • 5
    • 4
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 1484-1500
    适用
    • 125
    • 68
    • 47
    • 4
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    人, 小鼠
    宿主
    • 95
    • 38
    • 3
    • 1
    • 1
    克隆类型
    • 100
    • 38
    多克隆
    标记
    • 62
    • 10
    • 7
    • 6
    • 5
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    This Nestin antibody is un-conjugated
    应用范围
    • 71
    • 45
    • 41
    • 41
    • 40
    • 39
    • 33
    • 29
    • 25
    • 18
    • 9
    • 6
    • 2
    • 2
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC), ELISA
    Supplier Product No.
    600-401-417
    Supplier
    Rockland
    原理
    Nestin Antibody
    交叉反应 (详细)
    This affinity purified antibody is directed against human Nestin protein.
    产品特性
    Synonyms: rabbit anti-Nestin antibody, rabbit anti-NES antibody, Nbla00170
    纯化方法
    The product was affinity purified from monospecific antiserum by immunoaffinity purification.
    过滤
    Sterile filtered
    免疫原

    Immunogen: Anti-Nestin affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 1484-1500 of human Nestin protein.

    Immunogen Type: Conjugated Peptide

    亚型
    IgG
    Top Product
    Discover our top product NES Primary Antibody
  • 应用备注

    Immunohistochemistry Dilution: 5 μg/mL

    Application Note: Affinity purified Anti-Nestin has been tested for use in ELISA, Immunohistochemistry (IHC), and western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 200-220 kDa in size corresponding to Nestin protein by western blotting in the appropriate cell lysate or extract.

    Western Blot Dilution: 1:500 - 1:3,000

    ELISA Dilution: 1:20,000 - 1:80,000

    Other: User Optimized

    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    1.0 mg/mL
    缓冲液

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: None

    Preservative: 0.01 % (w/v) Sodium Azide
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    4 °C,-20 °C
    储存方法
    Store anti-Nestin at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
    有效期
    12 months
  • Mokrý, Cízková, Filip, Ehrmann, Osterreicher, Kolár, English: "Nestin expression by newly formed human blood vessels." in: Stem cells and development, Vol. 13, Issue 6, pp. 658-64, (2005) (PubMed).

    Aihara, Sugawara, Torii, Hosaka, Kurihara, Saito, Takeuchi: "Angiogenic endothelium-specific nestin expression is enhanced by the first intron of the nestin gene." in: Laboratory investigation; a journal of technical methods and pathology, Vol. 84, Issue 12, pp. 1581-92, (2005) (PubMed).

    Yaworsky, Kappen: "Heterogeneity of neural progenitor cells revealed by enhancers in the nestin gene." in: Developmental biology, Vol. 205, Issue 2, pp. 309-21, (1999) (PubMed).

    Dahlstrand, Zimmerman, McKay, Lendahl: "Characterization of the human nestin gene reveals a close evolutionary relationship to neurofilaments." in: Journal of cell science, Vol. 103 ( Pt 2), Issue 1, pp. 589-97, (1993) (PubMed).

    Hockfield, McKay: "Identification of major cell classes in the developing mammalian nervous system." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 5, Issue 12, pp. 3310-28, (1986) (PubMed).

  • 抗原
    Nestin (NES)
    别名
    NES (NES 产品)
    背景
    Background: Nestin, is a large intermediate filament protein (class Type VI) that was first identified with a monoclonal antibody by Hockfield and McKay (1985).  Nestin is expressed predominantly in stem cells of the central nervous system in the neural tube.  Nestin is expressed during development and in myotendinous and neuromuscular junctions. Nestin expression is restricted, typically disappearing by E18. Nestin is thought to be a reasonable neuronal marker, however, recent studies have found nestin expression in other cell types such as endothelial cells.  Nestin expression is seen in almost all GBMs (Glioblastoma multiformes) and many melanomas (both primary and metastatic) but not in any metastatic carcinomas.  Upon terminal neural differentiation, nestin is downregulated and replaced by neurofilaments.
    基因ID
    10763, 38176300
    UniProt
    P48681
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