Biotin 抗体 (Texas Red (TR))
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- 抗原 See all Biotin products
- Biotin
- 适用
- 请咨询
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This Biotin antibody is conjugated to Texas Red (TR)
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应用范围
- Fluorescence Microscopy (FM), Dot Blot (DB)
- Supplier Product No.
- 600-409-098
- Supplier
- Rockland
- 原理
- Biotin Antibody Texas Red™ Conjugated
- 交叉反应 (详细)
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Biotin conjugated IgG and Biotin conjugated Bovine Serum Albumin.
- 产品特性
- Synonyms: rabbit anti-Biotin Antibody Texas Red™ Conjugated, rabbit anti Biotin Antibody Texas Red™ Conjugated
- 纯化方法
- Rabbit Anti-Biotin was prepared from monospecific antiserum by immunoaffinity chromatography using Biotin coupled to sepharose beads.
- 免疫原
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Immunogen: Biotin conjugated to Keyhole Limpet Hemocyanin (KLH)
Immunogen Type: Other
- 亚型
- IgG
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- 应用备注
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Application Note: Anti-Biotin Texas Red Conjugated Antibody has been tested by Dot blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
IF Microscopy Dilution: 1:500-1:2,500
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
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Reconstitution Volume: 100 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- 缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Sodium Azide - 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- 有效期
- 12 months
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- 抗原
- Biotin
- Abstract
- Biotin 产品
- 物质类
- Chemical
- 背景
- Background: Biotin Antibody detects Biotin. Biotin is a water-soluble B-complex vitamin (vitamin B7). It is composed of a ureido (tetrahydroimidizalone) ring fused with a tetrahydrothiophene ring. A valeric acid substituent is attached to one of the carbon atoms of the tetrahydrothiophene ring. Biotin is a coenzyme for carboxylase enzymes, involved in the synthesis of fatty acids, isoleucine, and valine, and in gluconeogenesis. Biotin is necessary for cell growth, the production of fatty acids, and the metabolism of fats and amino acids. Anti-Biotin Texas Red Conjugated Antibody is ideal for investigators involved in Cell Signaling and Cell Biology research.
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