TUBA1B 抗体 (AA 425-451) (Biotin)
(1 reference)
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- 抗原 See all TUBA1B 抗体
- TUBA1B (Tubulin, alpha 1B (TUBA1B))
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抗原表位
- AA 425-451
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适用
- 人, 小鼠, 大鼠, 小鸡, Cow, 山羊, 绵羊
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This TUBA1B antibody is conjugated to Biotin
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应用范围
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Fluorescence Microscopy (FM)
- Supplier Product No.
- 600-406-880
- Supplier
- Rockland
- 原理
- Alpha-Tubulin Antibody Biotin Conjugated
- 交叉反应 （详细）
- Anti-Tubulin Loading Control Antibody is directed against human alpha Tubulin protein.
- 产品特性
- Synonyms: rabbit anti-alpha-Tubulin Antibody biotin conjugation, biotin conjugated rabbit anti-α-tubulin antibody, biotin conjugated rabbit anti-alpha-Tubulin Antibody, rabbit anti-α-tubulin antibody biotin conjugation, Tubulin alpha-1B chain, Tubulin alpha-ubiquitous chain, Alpha-tubulin ubiquitous, Tubulin K-alpha-1, TUBA1B, tubulin loading control, Alpha-tubulin, Tubulin alpha-1A, TUBA1A, TUBA3, LIS3
- 纯化方法
- The Loading Control Antibody was affinity purified from monospecific antiserum by immunoaffinity purification.
- 免疫原
- Optional[Immunogen]: Anti-Tubulin Loading Control Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the C-Terminal region near amino acids 425-451 of Human alpha Tubulin.
- 亚型
- IgG
- Top Product
- Discover our top product TUBA1B Primary Antibody
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anti-Tubulin, alpha 1B (TUBA1B) antibody
TUBA1B 适用: 人 WB, IF, IHC (p), ICC, IHC (wm) 宿主: 兔 Polyclonal unconjugated
anti-Tubulin, alpha 1B (TUBA1B) (AA 1-451) antibodyTUBA1B 适用: 人 WB, ELISA, IF, IHC (p) 宿主: 小鼠 Monoclonal 4D1 unconjugated
anti-Tubulin, alpha 1B (TUBA1B) (AA 1-451) antibodyTUBA1B 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Tubulin, alpha 1B (TUBA1B) (C-Term) antibodyTUBA1B 适用: 人 WB, ELISA, IHC, IF, FM, MA 宿主: 小鼠 Monoclonal 17H11-F10 unconjugated
anti-Tubulin, alpha 1B (TUBA1B) (AA 425-451) antibodyTUBA1B 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, FM 宿主: 兔 Polyclonal unconjugated
anti-Tubulin, alpha 1B (TUBA1B) (C-Term) antibodyTUBA1B 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Tubulin, alpha 1B (TUBA1B) (AA 29-310) antibodyTUBA1B 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Tubulin, alpha 1B (TUBA1B) (AA 1-451) antibodyTUBA1B 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 2E8 unconjugated
anti-Tubulin, alpha 1B (TUBA1B) (C-Term) antibodyTUBA1B 适用: 人, 小鼠, 大鼠, 小鸡, Cow WB, ELISA, IHC, IF, IHC (p) 宿主: 小鼠 Monoclonal 17H11-F10 unconjugated
anti-Tubulin, alpha 1B (TUBA1B) (AA 437-441) antibodyTUBA1B 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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Immunohistochemistry Dilution: 1:1,000-1:5,000
Application Note: Anti-Tubulin Loading Control Antibody has been tested for use in ELISA and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band at ~50 kDa in size corresponding to alpha tubulin by western blotting in the appropriate cell lysate or extract.
Western Blot Dilution: 1:2,000-1:10,000
ELISA Dilution: 1:20,000-1:100,000
IF Microscopy Dilution: 1:500 - 1:2,000
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
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Reconstitution Volume: 100 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- 浓度
- 0.61 mg/ml
- 缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Sodium Azide - 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store Anti-Tubulin Loading Control Antibody at 4° C prior to restoration. For extended storage aliquot Control Antibody and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge Tubulin Antibody if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- 有效期
- 12 months
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: "Structural features and restricted expression of a human alpha-tubulin gene." in: Nucleic acids research, Vol. 13, Issue 1, pp. 207-23, (1985) (PubMed).
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: "Structural features and restricted expression of a human alpha-tubulin gene." in: Nucleic acids research, Vol. 13, Issue 1, pp. 207-23, (1985) (PubMed).
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- 抗原
- TUBA1B (Tubulin, alpha 1B (TUBA1B))
- 别名
- TUBA1B (TUBA1B 产品)
- 背景
- Background: Microtubules are involved in a wide variety of cellular activities ranging from mitosis and transport events to cell movement and the maintenance of cell shape. Tubulin itself is a globular protein consisting of two polypeptides (alpha and beta tubulin). Alpha and beta tubulin dimers are assembled to 13 protofilaments that form a microtubule of 22-nm diameter. Tyrosine ligase adds a C-terminal tyrosine to monomeric alpha tubulin. Assembled microtubules can again be detyrosinated by a cytoskeleton-associated carboxypeptidase. Detyrosinated alpha tubulin is referred to as Glu-tubulin. Another post-translational modification of detyrosinated alpha tubulin is C-terminal polyglutamylation, which is characteristic of microtubules in neuronal cells and the mitotic spindle. This antibody makes an excellent loading control.
- UniProt
- P68363
- 途径
- Microtubule Dynamics, M Phase
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