GST 抗体 (FITC)
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- 抗原 See all GST 抗体
- GST (Glutathione S Transferase (GST))
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适用
- Schistosoma japonicum
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This GST antibody is conjugated to FITC
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应用范围
- Western Blotting (WB), ELISA, Flow Cytometry (FACS), Fluorescence Microscopy (FM)
- Supplier Product No.
- 600-402-200
- Supplier
- Rockland
- 原理
- GST Antibody Fluorescein Conjugated
- 交叉反应 (详细)
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-fluorescein, anti-Rabbit Serum as well as purified and partially purified Glutathione-S-Transferase [Schistosoma japonicum].
- 产品特性
- Synonyms: rabbit anti-GST antibody fluorescein conjugation, FITC conjugated rabbit anti-GST antibody, Glutathione-S-Transferase
- 纯化方法
- FITC conjugated GST Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using GST coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities and extensive dialysis against the buffer stated above.
- 免疫原
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Immunogen: The immunogen is full length GST isolated from Schistosoma japonicum.
Immunogen Type: Native Protein
- 亚型
- IgG
- Labeling Ratio
- 3.0
- Top Product
- Discover our top product GST Primary Antibody
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- 应用备注
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Flow Cytometry Dilution: User Optimized
Application Note: FITC conjugated GST Antibody has been tested by western blot and is ideal for microscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Western Blot Dilution: 1:2,000 - 1:10,000
ELISA Dilution: 1:60,000
IF Microscopy Dilution: User Optimized
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
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Reconstitution Volume: 100 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- 缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Sodium Azide - 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store FITC conjugated GST Antibody at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Antibody is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- 有效期
- 12 months
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- 抗原
- GST (Glutathione S Transferase (GST))
- 别名
- GST (GST 产品)
- 背景
- Background: GST (Glutathione-S-Transferase) is a protein expression tag commonly used in molecular biology. Anti-GST will react with synthetic construct present in most known GST containing cloning or expression vectors. GST is responsible for the conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. The amino acid sequence GST is highly conserved in most organisms including mammals. GST exists as a 26 kDa homodimer. This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
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