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NYS48/HAUS8 抗体 (Ser70)

Rockland 600-401-c53 HAUS8 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
Rockland
产品编号 ABIN1043902
Supplier Product No.: 600-401-c53
发货至: 中国
  • 抗原 See all NYS48/HAUS8 (HAUS8) 抗体
    NYS48/HAUS8 (HAUS8) (HAUS Augmin-Like Complex, Subunit 8 (HAUS8))
    抗原表位
    • 8
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Ser70
    适用
    • 24
    • 5
    • 2
    • 1
    宿主
    • 23
    • 2
    克隆类型
    • 25
    多克隆
    标记
    • 20
    • 1
    • 1
    • 1
    • 1
    • 1
    This NYS48/HAUS8 antibody is un-conjugated
    应用范围
    • 23
    • 13
    • 5
    • 4
    • 4
    • 3
    • 3
    • 2
    • 1
    • 1
    Western Blotting (WB), ELISA
    Supplier Product No.
    600-401-c53
    Supplier
    Rockland
    原理
    HICE1 Antibody
    交叉反应 (详细)
    Anti-Hice1 Affinity purified antibody is directed against human Hice1 protein.
    产品特性
    Synonyms: rabbit anti-HICE1 antibody, HICE-1, HICE 1, HAUS8, HAUS-8, HAUS 8, HAUS augmin-like complex subunit 8, HEC1/NDC80-interacting centrosome-associated protein 1, Sarcoma antigen NY-SAR-48
    纯化方法
    The product was affinity purified from monospecific antiserum by immunoaffinity purification.
    过滤
    Sterile filtered
    免疫原

    Immunogen: Anti-Hice1 Antibody was prepared by repeated immunizations with a synthetic peptide corresponding to the region of amino acids containing serine 70 of human Hice1.

    Immunogen Type: Conjugated Peptide

    亚型
    IgG
    Top Product
    Discover our top product HAUS8 Primary Antibody
  • 应用备注

    Application Note: Hice1 antibody has been tested for use in ELISA and by western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 44.9 kDa in size corresponding to human Hice1 protein by western blotting in the appropriate stimulated tissue or cell lysate or extract.

    Western Blot Dilution: 0.45 μg/mL

    ELISA Dilution: 1:30,000-1:45,000

    Other: User Optimized

    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    0.45 mg/mL
    缓冲液

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: None

    Preservative: 0.01 % (w/v) Sodium Azide
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    4 °C,-20 °C
    储存方法
    Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
    有效期
    12 months
  • Wu, Lin, Wei, Chen, Shan, Lee: "Hice1, a novel microtubule-associated protein required for maintenance of spindle integrity and chromosomal stability in human cells." in: Molecular and cellular biology, Vol. 28, Issue 11, pp. 3652-62, (2008) (PubMed).

  • 抗原
    NYS48/HAUS8 (HAUS8) (HAUS Augmin-Like Complex, Subunit 8 (HAUS8))
    别名
    HAUS8 (HAUS8 产品)
    背景
    Background: Hice1 is designed, produced, and validated as part of a collaboration with the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. Hice1 contributes to the mitotic spindle assembly, maintenance of centrosome integrity and completion of cytokinesis as part of the HAUS augmin-like complex. Normal bipolar spindle formation is critical for accurate chromosome segregation and proper mitotic progression. Failure in this event leads to spindle checkpoint activation and chromosome missegregation that ultimately leads to aneuploidy. Hice1 binds to microtubules directly, and promotes spindle integrity and chromosome stability. Hice1 has also shown to play an important role in targeting the gamma TuRC complex to the mitotic spindle, a step that appears to be required for spindle-mediated microtubule generation and normal chromosome segregation. The HAUS augmin-like complex's interaction with microtubules is strong during mitosis, while it is weak or absent during interphase. During interphase, it is primarily cytoplasmic, associating with centrosomes and with the mitotic spindles, preferentially at the spindle pole vicinity. During anaphase and telophase, it additionally associates with the spindle midzone and midbody, respectively. Further characterization of the function of Hice1 will likely be important for better understanding the mechanism of normal mitotic progression and high fidelity chromosome segregation.
    基因ID
    93323
    NCBI登录号
    NP_219485
    UniProt
    Q9BT25
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