(3 references)-
- 抗原 See all Myc Tag products
- Myc Tag
- 适用
- 请咨询
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This Myc Tag antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunoprecipitation (IP), ELISA
- 序列
- EQKLISEEDL
- 产品特性
- Concentration Definition: by UV absorbance at 280 nm
- 免疫原
- This antibody was purified from whole rabbit serum prepared by repeated immunizations with Myc epitope tag peptide, E-Q-K-L-I-S-E-E-D-L, conjugated to KLH using maleimide. The sequence corresponds to amino acids 410-419 of human c-Myc.
- 亚型
- IgG
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anti-Myc Tag antibody
WB, IP, ELISA, IF 宿主: 小鼠 Monoclonal unconjugated
anti-Myc Tag (C-Term) antibody适用: 人 WB, IP, IHC (p), FACS 宿主: 小鼠 Monoclonal 9E10 unconjugated
anti-Myc Tag (C-Term) antibody (FITC)适用: 人 FACS 宿主: 小鼠 Monoclonal 9E10 FITC
anti-Myc Tag (C-Term) antibody (Biotin)适用: 人 WB, IP, IHC (p), FACS 宿主: 小鼠 Monoclonal 9E10 Biotin
anti-Myc Tag antibodyWB, IP, ELISA, IF 宿主: 小鼠 Monoclonal 2G8D5 unconjugated
anti-Myc Tag (AA 408-439) antibodyWB, ELISA 宿主: 小鼠 Monoclonal 9E10- unconjugated
anti-Myc Tag antibodyWB, IP, IF 宿主: 小鼠 Monoclonal 3B8 unconjugated
anti-Myc Tag (AA 410-419) antibodyWB 宿主: 山羊 Polyclonal unconjugated
anti-Myc Tag (AA 408-439) antibody适用: 人 WB, IP, IF, IHC (fro), IHC (p) 宿主: 小鼠 Monoclonal 9E10 unconjugated Recombinant Antibody
anti-Myc Tag (AA 410-419) antibodyWB, IP, IF, IHC, ICC 宿主: 小鼠 Monoclonal Myc-A7 unconjugated
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- 应用备注
- Anti-Myc has utility to detect the fusion protein of the Myc epitope cloned along with the target gene. As such, anti-Myc/Myc can be used to identify fusion proteins containing the Myc epitope. The antibody recognizes the Myc tag fused either to the AMINO- or CARBOXY- termini of targeted proteins. This antibody is suitable for ELISA and western blotting and was tested against both the immunizing peptide and Myc-tagged recombinant proteins. Although not tested, this antibody is likely functional for immunoprecipitation and immunocytochemistry.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1.0 mg/mL
- 缓冲液
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- -20 °C
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: "SARS-Coronavirus Open Reading Frame-3a drives multimodal necrotic cell death." in: Cell death & disease, Vol. 9, Issue 9, pp. 904, (2018) (PubMed).
: "Specific dephosphorylation at tyr-554 of git1 by ptprz promotes its association with paxillin and hic-5." in: PLoS ONE, Vol. 10, Issue 3, pp. e0119361, (2016) (PubMed).
: "γ-secretase-dependent cleavage initiates notch signaling from the plasma membrane." in: Traffic (Copenhagen, Denmark), Vol. 11, Issue 9, pp. 1234-45, (2011) (PubMed).
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: "SARS-Coronavirus Open Reading Frame-3a drives multimodal necrotic cell death." in: Cell death & disease, Vol. 9, Issue 9, pp. 904, (2018) (PubMed).
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- 抗原
- Myc Tag
- Abstract
- Myc Tag 产品
- 物质类
- Tag
- 背景
- Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the biochemical properties of the tagged protein. Most often, sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag-containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures. Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells. Supplier produces anti-epitope tag antibodies against many common epitope tags including Myc, GST, GFP, 6X His, MBP, FLAG and HA. Supplier also produces antibodies to other tags including FITC, Rhodamine (TRITC), DNP and biotin.
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