IL-18 抗体 (AA 1-157)
(3 references)-
- 抗原 See all IL-18 (IL18) 抗体
- IL-18 (IL18) (Interleukin 18 (IL18))
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抗原表位
- AA 1-157
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适用
- 小鼠
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This IL-18 antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA, Immunoprecipitation (IP), Flow Cytometry (FACS), Radioimmunoassay (RIA), Neutralization (Neut)
- 特异性
- This is an IgG preparation of whole rabbit serum purified by protein A chromatography using a low endotoxin methodology. This antibody is primarily directed against mature 18,000 MW mouse IL-18 and is useful in determining its presence in various assays. This antibody will also recognize the 24,000 inactive precursor form of mouse IL-18. In general, this antibody also detects rat IL-18 in the same formats using similar dilutions. A control of similarly diluted LOW ENDOTOXIN CONTROL RABBIT IgG (code # 011-001-297) is recommended.
- 产品特性
- Interleukin-18 (IL-18) is a member of the IL-1 cytokine family and was initially identified as an Interferon-g (IFN-g) inducing factor (IGIF). The IL-18 gene was originally cloned from liver cells and has since been shown to be produced by activated monocytes/ macrophages, Kupffer cells, keratinocytes, glucocorticoid-secreting adrenal cortex cells, osteoblasts and dendritic cells. IL-18 is a 24 kDa, non-glycosylated polypeptide that lacks a classical signal sequence and possesses a structure recognizably similar to IL-1. IL-18 is synthesized as a bio-inactive propeptide that undergoes proteolytic cleavage by either ICE (interleukin-1 beta converting enzyme) or another caspase to generate a mature, bioactive, 18 kDa molecule. In both the mature and propeptide forms, IL-18 shows 64% aa sequence identity from mouse to human. IL-18 does not appear to show any primary sequence similarity to any other known cytokines. Rat IL-18 has also been isolated, and found to be 194 aa in length with a 91% aa sequence identity to mouse IL-18. Human IL-18 has been found to induce the production of IFN-g and GM-CSF while inhibiting the production of IL-10 by PBMC. With respect to human T cells, IL-18 enhances Th1 cytokine production and stimulates cell proliferation via an IL-2-dependent pathway. Human IL-18 can also inhibit the synthesis of IgE by B cells. Thus, IL-18 plays an important role in immunological and inflammatory reactions. Currently, the bioactivity of human IL-18 is often determined by its capacity to augment the levels of IFN-g produced by T cells as measured in tissue culture supernatants.
- 过滤
- Sterile filtered
- 免疫原
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The whole rabbit serum used to produce this IgG fraction antibody was prepared by repeated immunizations with native 157 aa mouse IL-18 produced in E.coli.
Immunogen Type: Recombinant Protein - 亚型
- IgG
- Top Product
- Discover our top product IL18 Primary Antibody
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anti-Interleukin 18 (IL18) (AA 37-194) antibody
IL18 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 18 (IL18) (AA 155-188), (C-Term) antibodyIL18 适用: 人 WB 宿主: 兔 Polyclonal RB48154 unconjugated
anti-Interleukin 18 (IL18) (AA 37-193) antibodyIL18 适用: 人 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal C14 unconjugated
anti-Interleukin 18 (IL18) (Internal Region) antibodyVerified IL18 适用: 人 WB, ELISA, IF, FACS 宿主: 山羊 Polyclonal unconjugated
anti-Interleukin 18 (IL18) (AA 37-193) antibodyIL18 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 18 (IL18) (AA 36-192) antibodyIL18 适用: Pig WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 18 (IL18) (AA 94-193) antibodyIL18 适用: 人 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal C5 unconjugated
anti-Interleukin 18 (IL18) (AA 32-192) antibodyIL18 适用: 兔 WB, IHC, IP, ICC 宿主: 豚鼠 Polyclonal unconjugated
anti-Interleukin 18 (IL18) antibodyIL18 适用: 人, 小鼠, 大鼠 IF 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 18 (IL18) (AA 32-192) antibodyIL18 适用: 兔 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal C3 unconjugated
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- 应用备注
- This IgG fraction antibody of anti-Mouse IL-18 has been tested for use in neutralizations, ELISA, immunohistochem-istry and immunoblotting. Although untested, this reagent may be useful for radioimmunoassays, flow cytometry and immunoprecip-itation. It recognizes the 18,000 MW mature (active) IL-18. Reactivity in other immunoassays is unknown.
- 说明
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Gene Name: IL18
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1.0 mg/mL
- 缓冲液
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
- 储存液
- Without preservative
- 储存条件
- 4 °C/-20 °C
- 储存方法
- Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -20 °C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.
- 有效期
- 12 months
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: "A truncating mutation in the autophagy gene UVRAG drives inflammation and tumorigenesis in mice." in: Nature communications, Vol. 10, Issue 1, pp. 5681, (2020) (PubMed).
: "Extracellular TDP-43 aggregates target MAPK/MAK/MRK overlapping kinase (MOK) and trigger caspase-3/IL-18 signaling in microglia." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 31, Issue 7, pp. 2797-2816, (2017) (PubMed).
: "TRAF3IP2 mediates aldosterone/salt-induced cardiac hypertrophy and fibrosis." in: Molecular and cellular endocrinology, Vol. 429, pp. 84-92, (2016) (PubMed).
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: "A truncating mutation in the autophagy gene UVRAG drives inflammation and tumorigenesis in mice." in: Nature communications, Vol. 10, Issue 1, pp. 5681, (2020) (PubMed).
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- 抗原
- IL-18 (IL18) (Interleukin 18 (IL18))
- 别名
- IL-18 (IL18 产品)
- 别名
- Igif antibody, Il-18 antibody, IL-18 antibody, IGIF antibody, ChIL-18 antibody, IL-1g antibody, IL1F4 antibody, IL18 antibody, interleukin 18 antibody, Il18 antibody, IL18 antibody
- 背景
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Interleukin-18 (IL-18) is a member of the IL-1 cytokine family and was initially identified as an Interferon-g (IFN-g) inducing factor (IGIF). The IL-18 gene was originally cloned from liver cells and has since been shown to be produced by activated monocytes/ macrophages, Kupffer cells, keratinocytes, glucocorticoid-secreting adrenal cortex cells, osteoblasts and dendritic cells. IL-18 is a 24 kDa, non-glycosylated polypeptide that lacks a classical signal sequence and possesses a structure recognizably similar to IL-1. IL-18 is synthesized as a bio-inactive propeptide that undergoes proteolytic cleavage by either ICE (interleukin-1 beta converting enzyme) or another caspase to generate a mature, bioactive, 18 kDa molecule. In both the mature and propeptide forms, IL-18 shows 64% aa sequence identity from mouse to human. IL-18 does not appear to show any primary sequence similarity to any other known cytokines. Rat IL-18 has also been isolated, and found to be 194 aa in length with a 91% aa sequence identity to mouse IL-18. Human IL-18 has been found to induce the production of IFN-g and GM-CSF while inhibiting the production of IL-10 by PBMC. With respect to human T cells, IL-18 enhances Th1 cytokine production and stimulates cell proliferation via an IL-2-dependent pathway. Human IL-18 can also inhibit the synthesis of IgE by B cells. Thus, IL-18 plays an important role in immunological and inflammatory reactions. Currently, the bioactivity of human IL-18 is often determined by its capacity to augment the levels of IFN-g produced by T cells as measured in tissue culture supernatants.
Synonyms: Iboctadekin antibody, IFN gamma inducing factor antibody, IGIF antibody, IL 1 gamma antibody, IL 18 antibody, IL 1g antibody, IL-1F4, IL 18, Interleukin 18, IL18, Interleukin18, IL1 F4, IL1F4 - 基因ID
- 16173
- UniProt
- P70380
- 途径
- Cellular Response to Molecule of Bacterial Origin, Activated T Cell Proliferation, Cancer Immune Checkpoints, Inflammasome
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