VEGFA 抗体 (Biotin)
(3 references)
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- 抗原 See all VEGFA 抗体
- VEGFA (Vascular Endothelial Growth Factor A (VEGFA))
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适用
- 人
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This VEGFA antibody is conjugated to Biotin
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应用范围
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
- Supplier Product No.
- 209-406-b99
- Supplier
- Rockland
- 原理
- VEGF Antibody Biotin Conjugated
- 交叉反应 （详细）
- This purified antibody has been heated to 56 °C for 30 minutes.
- 产品特性
- Synonyms: rabbit anti-VEGF Biotin Conjugated antibody, rabbit anti-VEGF-165 Biotin Conjugated antibody, Vascular endothelial growth factor A, VEGF-A, VEGF-165, VEGF165, VEGF isoform L, Vascular permeability factor, VPF
- 纯化方法
- Biotin Conjugated VEGF Antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
- 免疫原
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Immunogen: VEGF Antibody Biotin Conjugated was prepared from whole rabbit serum produced by repeated immunizations with full length recombinant human VEGF-165 protein.
Immunogen Type: Recombinant Protein
- 亚型
- IgG
- Top Product
- Discover our top product VEGFA Primary Antibody
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anti-Vascular Endothelial Growth Factor A (VEGFA) (AA 1-147) antibody
VEGFA 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Vascular Endothelial Growth Factor A (VEGFA) (AA 28-147) antibodyVEGFA 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Vascular Endothelial Growth Factor A (VEGFA) (AA 27-190) antibodyVEGFA 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Vascular Endothelial Growth Factor A (VEGFA) (AA 27-214) antibodyVEGFA 适用: 大鼠 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal C2 unconjugated
anti-Vascular Endothelial Growth Factor A (VEGFA) antibodyVEGFA 适用: 小鼠 WB, ELISA, Neut 宿主: 兔 Polyclonal unconjugated
anti-Vascular Endothelial Growth Factor A (VEGFA) (AA 27-126) antibodyVEGFA 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Vascular Endothelial Growth Factor A (VEGFA) (AA 207-371) antibodyVEGFA 适用: 人 WB, ELISA, ICC 宿主: 小鼠 Monoclonal 6G5B5 unconjugated
anti-Vascular Endothelial Growth Factor A (VEGFA) (AA 27-191) antibodyVEGFA 适用: 人, 大鼠 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Vascular Endothelial Growth Factor A (VEGFA) (AA 33-48), (N-Term) antibodyVEGFA 适用: 人, 大鼠, 小鼠 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Vascular Endothelial Growth Factor A (VEGFA) (AA 27-130) antibodyVEGFA 适用: 人 WB, ELISA, PLA 宿主: 小鼠 Monoclonal 3F7 unconjugated
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- 应用备注
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Immunohistochemistry Dilution: 1:500-1:2,500
Application Note: VEGF conjugated antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 22 kDa in size corresponding to monomeric human VEGF-165 protein by western blotting in the appropriate cell lysate or extract.
Western Blot Dilution: 1:2,000 - 1:10,000
ELISA Dilution: 1:10,000 - 1:50,000
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
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Reconstitution Volume: 100 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- 缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Sodium Azide - 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store Biotin Conjugated VEGF Antibody at 4° C prior to restoration. For extended storage aliquot Antibody and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- 有效期
- 12 months
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: "Deregulation of EGFR/VEGF/HIF-1a signaling pathway in colon adenocarcinoma based on tissue microarrays analysis." in: Journal of B.U.ON. : official journal of the Balkan Union of Oncology, Vol. 15, Issue 1, pp. 107-15, (2010) (PubMed).
: "Brain-derived neurotrophic factor induces proliferation, migration, and VEGF secretion in human multiple myeloma cells via activation of MEK-ERK and PI3K/AKT signaling." in: Tumour biology, Vol. 31, Issue 2, pp. 121-8, (2010) (PubMed).
: "New vascular endothelial growth factor isoform generated by internal ribosome entry site-driven CUG translation initiation." in: Molecular endocrinology (Baltimore, Md.), Vol. 15, Issue 12, pp. 2197-210, (2001) (PubMed).
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: "Deregulation of EGFR/VEGF/HIF-1a signaling pathway in colon adenocarcinoma based on tissue microarrays analysis." in: Journal of B.U.ON. : official journal of the Balkan Union of Oncology, Vol. 15, Issue 1, pp. 107-15, (2010) (PubMed).
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- 抗原
- VEGFA (Vascular Endothelial Growth Factor A (VEGFA))
- 别名
- VEGFA (VEGFA 产品)
- 背景
- Background: VEGF (Vascular Endothelial Growth Factor A) is a homodimeric, disulfide-linked glycoprotein involved in angiogenesis which promotes tumor progression and metastasis. It exhibits potent mitogenic and permeability inducing properties specific for the vascular endothelium. Of the four isoforms of VEGF, the smaller two, VEGF 165 and VEGF 121, are secreted proteins and act as diffusible agents, whereas the larger two (VEGF 189 and VEGF 206) remain cell associated. The sequence of this isoform differs from the canonical sequence as follows: 141-141: K → N and 142-182: missing. This isoform is often found as a disulfide linked homodimer.
- 基因ID
- 7422
- NCBI登录号
- NP_001165097
- UniProt
- P15692
- 途径
- RTK signaling, Glycosaminoglycan Metabolic Process, Regulation of Cell Size, Tube Formation, Signaling Events mediated by VEGFR1 and VEGFR2, Platelet-derived growth Factor Receptor Signaling, VEGFR1 Specific Signals, VEGF Signaling
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