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AKT2 抗体 (Internal Region)

Name: AKT2 抗体 (Internal Region)
Brand: antibodies-online
SKU: ABIN1043774
Availability: OutOfStock

AKT2 适用: 人 WB, ELISA, IHC 宿主: 大鼠 Monoclonal 16G11-A7 unconjugated

Western Blot of Rat Anti-AKT2 antibody. (AKT2 抗体 (Internal Region))

Flow Cytometry of Rat anti-AKT2 antibody. (AKT2 抗体 (Internal Region))

FACS

Immunohistochemistry of Rat monoclonal anti AKT2 Antibody in human breast carcinoma. (AKT2 抗体 (Internal Region))

IHC

4 images

(1 reference)

产品编号 ABIN1043774

发货至: 中国

Datasheet as PDF

抗原 See all AKT2 抗体

AKT2 (V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2))
抗原表位
22

21

17

15

15

15

12

8

7

5

5

5

4

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1
Internal Region
适用
180

99

65

17

14

14

13

13

10

9

9

9

6

4

4

3

2

1
人
宿主
161

27

4

2

1

1
大鼠
Compatible Secondaries
克隆类型
163

31
单克隆
标记
95

16

16

11

9

9

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1
This AKT2 antibody is un-conjugated
应用范围
166

101

73

35

28

26

26

18

17

9

8

8

7

6

4

3

2

1

1
Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

特异性

Anti-AKT2 antibody is directed against human AKT2. The antibody detects both unphosphorylated and phosphorylated forms of the protein. Anti-AKT2 was purified from ascites by Protein A chromatography. Cross reactivity with AKT2 from other species has not been determined, however, the sequence of the immunogen shows 88% identity to mouse and 90% identity with rat, therefore, cross reactivity is expected.

产品特性

AKT2 Antibody detects AKT2 which is a component of the PI-3 kinase pathway and is activated by phosphorylation at Ser 473 and Thr 308. AKT is a cytoplasmic protein also known as Protein Kinase B (PKB) and rac (related to A and C kinases). AKT is a key regulator of many signal transduction pathways. AKT Exhibits tight control over cell proliferation and cell viability. Overexpression or inappropriate activation of AKT is noted in many types of cancer. AKT mediates many of the downstream events of PI 3-kinase (a lipid kinase activated by growth factors, cytokines and insulin). PI 3-kinase recruits AKT to the membrane, where it is activated by PDK1 phosphorylation. Once phosphorylated, AKT dissociates from the membrane and phosphorylates targets in the cytoplasm and the cell nucleus. AKT has two main roles: (i) inhibition of apoptosis, (ii) promotion of proliferation. Anti-AKT2 Antibody is ideal for investigators involved in Cell Signaling, Neuroscience, Signal Transduction research.

过滤

Sterile filtered

免疫原

Anti-AKT2 Antibody was produced by repeated immunizations with a synthetic peptide corresponding to internal residues of human AKT2 protein.
Immunogen Type: Peptide

克隆位点

16G11-A7

亚型

IgG2a kappa

Top Product

Discover our top product AKT2 Primary Antibody
anti-V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2) (AA 100-189) antibody
AKT2 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 1D9 unconjugated

anti-V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2) (AA 100-189) antibody
AKT2 适用: 人 WB, ELISA, IF, IHC (p) 宿主: 小鼠 Monoclonal X1 unconjugated

anti-V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2) (AA 100-189) antibody
AKT2 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 1F8 unconjugated

anti-V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2) (AA 93-123) antibody
AKT2 适用: 人 WB, ELISA, IHC, IF, FACS 宿主: 兔 Polyclonal unconjugated

anti-V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2) (AA 416-444) antibody
AKT2 适用: 人 WB, ELISA, IHC, IF, FACS 宿主: 兔 Polyclonal unconjugated

anti-V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2) (AA 142-412) antibody
AKT2 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2) (C-Term) antibody
AKT2 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2) (pSer474) antibody
AKT2 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2) antibody
AKT2 适用: 人 WB, FACS, IP 宿主: 小鼠 Monoclonal 8D9 unconjugated

anti-V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2) antibody
AKT2 适用: 人, 大鼠, 猴 WB, ELISA, IHC, ICC 宿主: 小鼠 Monoclonal 1B6 unconjugated

应用备注

Anti-AKT2 Antibody is suitable for ELISA, immunohistochemistry, and western blotting. Expect a band approximately 56 kDa in size corresponding to AKT2 protein by western blotting in the appropriate cell lysate or extract. This monoclonal antibody reacts with human AKT. Specific conditions for reactivity should be optimized by the end user. For immunohistochemistry we recommend the use of fresh frozen tissues. Attempts at staining paraffin-embedded formalin fixed tissues were negative. No pre-treatment of sample is required.

说明

Gene Name: AKT2

限制

仅限研究用
状态

Liquid

缓冲液

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

储存液

Sodium azide

注意事项

This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C/-20 °C

储存方法

Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -20 °C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.

有效期

12 months
Altomare, Kozak, Sonoda, Testa: "Chromosome mapping of the mouse Akt2 gene and Akt2 pseudogene." in: Cytogenetics and cell genetics, Vol. 74, Issue 4, pp. 248-51, (1997) (PubMed).
抗原

AKT2 (V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2))

别名

AKT2 (AKT2 产品)

别名

AKT2 antibody, pkbb antibody, pkbbeta antibody, pkbss antibody, prkbb antibody, rac-beta antibody, v-akt2 antibody, 2410016A19Rik antibody, AW554154 antibody, PKB antibody, PKBbeta antibody, HIHGHH antibody, PKBB antibody, PKBBETA antibody, PRKBB antibody, RAC-BETA antibody, cb945 antibody, akt2-a antibody, AKT serine/threonine kinase 2 antibody, v-akt murine thymoma viral oncogene homolog 2 antibody, thymoma viral proto-oncogene 2 antibody, v-akt murine thymoma viral oncogene homolog 2 L homeolog antibody, AKT2 antibody, akt2 antibody, Akt2 antibody, akt2.L antibody

背景

AKT2 Antibody detects AKT2 which is a component of the PI-3 kinase pathway and is activated by phosphorylation at Ser 473 and Thr 308. AKT is a cytoplasmic protein also known as Protein Kinase B (PKB) and rac (related to A and C kinases). AKT is a key regulator of many signal transduction pathways. AKT Exhibits tight control over cell proliferation and cell viability. Overexpression or inappropriate activation of AKT is noted in many types of cancer. AKT mediates many of the downstream events of PI 3-kinase (a lipid kinase activated by growth factors, cytokines and insulin). PI 3-kinase recruits AKT to the membrane, where it is activated by PDK1 phosphorylation. Once phosphorylated, AKT dissociates from the membrane and phosphorylates targets in the cytoplasm and the cell nucleus. AKT has two main roles: (i) inhibition of apoptosis; (ii) promotion of proliferation. Anti-AKT2 Antibody is ideal for investigators involved in Cell Signaling, Neuroscience, Signal Transduction research.
Synonyms: AKT 2 antibody, AKT-2, PKB antibody, PKB beta antibody, PKBBETA antibody, PRKBB antibody, Protein kinase Akt 2 antibody, Protein kinase B beta antibody, RAC-beta serine/threonine-protein kinase, RAC-PK-beta

基因ID

208

UniProt

P31751

途径

PI3K-Akt Signaling, RTK signaling, AMPK Signaling, TLR signaling, Cellular Glucan Metabolic Process, Regulation of Carbohydrate Metabolic Process, Hepatitis C, VEGF Signaling