P35 抗体
(1 reference)
-
- 抗原
- P35
-
适用
- Borrelia burgdorferi
-
宿主
- 兔
-
克隆类型
- 多克隆
-
标记
- 非结合性
-
应用范围
- Western Blotting (WB), ELISA
- Supplier Product No.
- 200-401-c12
- Supplier
- Rockland
- 原理
- p35 Antibody
- 交叉反应 （详细）
- This antibody is specific for Borrelia burgdorferi p35 protein.
- 产品特性
- Synonyms: rabbit anti-p35 Antibody, bba64, Borrelia burgdorferi p35
- 纯化方法
- This product was Protein-A purified and cross-adsorbed against MBP from monospecific antiserum by chromatography.
- 免疫原
-
Immunogen: MBP-fusion protein corresponding to Borrelia burgdorferi p35 protein.
Immunogen Type: Recombinant Protein
- 亚型
- IgG
-
anti-P35 (N-Term) antibody
适用: 人, 小鼠, 大鼠 WB, ELISA, IF, IHC 宿主: 兔 Polyclonal unconjugated
-
- 应用备注
-
Application Note: This protein-A purified antibody has been tested for use in Western blotting and ELISA. Specific conditions for reactivity should be optimized by the user. Expect a band approximately 27.1 kDa in size corresponding to Borrelia burgdorferi OspA protein by Western blotting in the appropriate cell lysate or extract.
Western Blot Dilution: 1:1,000
ELISA Dilution: >1:5,000
Other: User Optimized
- 限制
- 仅限研究用
-
- 状态
- Lyophilized
- 溶解方式
-
Reconstitution Volume: 100 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- 浓度
- 1.0 mg/mL
- 缓冲液
-
Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide - 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- 有效期
- 12 months
-
-
: "Analysis of the determinants of bba64 (P35) gene expression in Borrelia burgdorferi using a gfp reporter." in: Microbiology (Reading, England), Vol. 154, Issue Pt 1, pp. 275-85, (2008) (PubMed).
-
: "Analysis of the determinants of bba64 (P35) gene expression in Borrelia burgdorferi using a gfp reporter." in: Microbiology (Reading, England), Vol. 154, Issue Pt 1, pp. 275-85, (2008) (PubMed).
-
- 抗原
- P35
- 背景
- Background: The p35 kDa protein of the spirochete Borrelia burgdorferi is being investigated for use as an early diagnostic marker of Lyme Disease. Borrelia may change its antigenic composition in its need for adaptation to stresses imposed by changes in conditions from cycling between its arthropod and mammalian hosts. This group of B. burgdorferi proteins may be induced in the tick midgut during the feeding event. The p35 protein elicits a protective immunity from wild type B. burgdorferi. It has been shown that p35 expression in B. burgdorferi is upregulated in the stationary growth phase, and that a temperature of 34 °C but not 24 °C influenced the expression. The expression of a majority of the proteins expressed in early Lyme disease is affected pH , being abundantly expressed at pH 7.0 (resembling the tick midgut pH of 6.8 during feeding) but only sparsely at pH 8.0 (a condition closer to that of the unfed tick midgut pH of 7.4). The encoding genes may be coregulated. The 35- kDa antigen has been shown to be a statistically significant marker in IgG immunoblots in a study of patients with early Lyme disease who presented with erythema migrans. Recombinant p35 protein may be useful as a diagnostic reagent, especially in combination with other antigens that have been deemed relevant in serodiagnosis of early Lyme disease.
- 基因ID
- 1194146
- NCBI登录号
- WP_010256558
- UniProt
- O50687
-
