P35 抗体
适用: Borrelia burgdorferi
WB, ELISA, LF
宿主: 兔
Polyclonal
unconjugated
(1 reference)-
- 抗原
- P35
-
适用
- Borrelia burgdorferi
-
宿主
- 兔
-
克隆类型
- 多克隆
-
标记
- 非结合性
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应用范围
- Western Blotting (WB), ELISA, Lateral Flow (LF)
- 特异性
- This product was Protein-A purified and cross-adsorbed against MBP from monospecific antiserum by chromatography. This antibody is specific for Borrelia burgdorferi p35 protein. A BLAST analysis was used to suggest cross-reactivity with p35 from B. burgdorferi, garinii, and afzelii sources based on 100% homology with the immunizing sequence. Cross-reactivity with p35 from other sources has not been determined.
- 产品特性
- The p35 kDa protein of the spirochete Borrelia burgdorferi is being investigated for use as an early diagnostic marker of Lyme Disease. Borrelia may change its antigenic composition in its need for adaptation to stresses imposed by changes in conditions from cycling between its arthropod and mammalian hosts. This group of B. burgdorferi proteins may be induced in the tick midgut during the feeding event. The p35 protein elicits a protective immunity from wild type B. burgdorferi. It has been shown that p35 expression in B. burgdorferi is upregulated in the stationary growth phase, and that a temperature of 34°C but not 24°C influenced the expression. The expression of a majority of the proteins expressed in early Lyme disease is affected pH, being abundantly expressed at pH 7.0 (resembling the tick midgut pH of 6.8 during feeding) but only sparsely at pH 8.0 (a condition closer to that of the unfed tick midgut pH of 7.4). The encoding genes may be coregulated. The 35-kDa antigen has been shown to be a statistically significant marker in IgG immunoblots in a study of patients with early Lyme disease who presented with erythema migrans. Recombinant p35 protein may be useful as a diagnostic reagent, especially in combination with other antigens that have been deemed relevant in serodiagnosis of early Lyme disease.
- 纯化方法
- purified
- 免疫原
-
MBP-fusion protein corresponding to Borrelia burgdorferi p35 protein.
Immunogen Type: RecombinantProtein - 亚型
- IgG
-
anti-P35 (N-Term) antibody
适用: 人, 小鼠, 大鼠 WB, ELISA, IF, IHC 宿主: 兔 Polyclonal unconjugated
-
- 应用备注
- This protein-A purified antibody has been tested for use in Western blotting and ELISA. Specific conditions for reactivity should be optimized by the user. Expect a band approximately 27.1 kDa in size corresponding to Borrelia burgdorferi OspA protein by Western blotting in the appropriate cell lysate or extract.
- 说明
-
Gene Name: BB-H32, bba64
- 限制
- 仅限研究用
-
- 状态
- Lyophilized
- 溶解方式
- Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 100 µL
- 浓度
- 1.0 mg/mL
- 缓冲液
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C/-20 °C
- 储存方法
- Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -20 °C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.
- 有效期
- 12 months
-
-
: "Analysis of the determinants of bba64 (P35) gene expression in Borrelia burgdorferi using a gfp reporter." in: Microbiology (Reading, England), Vol. 154, Issue Pt 1, pp. 275-85, (2008) (PubMed).
-
: "Analysis of the determinants of bba64 (P35) gene expression in Borrelia burgdorferi using a gfp reporter." in: Microbiology (Reading, England), Vol. 154, Issue Pt 1, pp. 275-85, (2008) (PubMed).
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- 抗原
- P35
- 别名
- Anx1 antibody, p35 antibody, CDK5P35 antibody, CDK5R antibody, NCK5A antibody, p23 antibody, p25 antibody, p35nck5a antibody, 2010323F13Rik antibody, GFRP antibody, P35 antibody, annexin A1 antibody, cyclin dependent kinase 5 regulatory subunit 1 antibody, GTP cyclohydrolase I feedback regulator antibody, Anxa1 antibody, CDK5R1 antibody, Gchfr antibody
- 物质类
- Viral Protein
- 背景
-
The p35 kDa protein of the spirochete Borrelia burgdorferi is being investigated for use as an early diagnostic marker of Lyme Disease. Borrelia may change its antigenic composition in its need for adaptation to stresses imposed by changes in conditions from cycling between its arthropod and mammalian hosts. This group of B. burgdorferi proteins may be induced in the tick midgut during the feeding event. The p35 protein elicits a protective immunity from wild type B. burgdorferi. It has been shown that p35 expression in B. burgdorferi is upregulated in the stationary growth phase, and that a temperature of 34°C but not 24°C influenced the expression. The expression of a majority of the proteins expressed in early Lyme disease is affected pH, being abundantly expressed at pH 7.0 (resembling the tick midgut pH of 6.8 during feeding) but only sparsely at pH 8.0 (a condition closer to that of the unfed tick midgut pH of 7.4). The encoding genes may be coregulated. The 35-kDa antigen has been shown to be a statistically significant marker in IgG immunoblots in a study of patients with early Lyme disease who presented with erythema migrans. Recombinant p35 protein may be useful as a diagnostic reagent, especially in combination with other antigens that have been deemed relevant in serodiagnosis of early Lyme disease.
Synonyms: bba64, Borrelia burgdorferi p35 - 基因ID
- 1194146
- NCBI登录号
- NP_045522
- UniProt
- O50687
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