Ubiquitin 抗体
(1 reference)-
- 抗原 See all Ubiquitin 抗体
- Ubiquitin
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适用
- Various Species
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This Ubiquitin antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP)
- 特异性
- This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum.
- 产品特性
- Ubiquitin (Ub) is a small, 76-residue, protein (8.5 kDa) found both as free monomer and covalently attached to itself and other proteins in eukaryotic cells. Free Ub is a very compact and stable molecule that is easily refolded after being denatured. It is therefore recommended that for detection of free Ub on Westerns, the Tris-Tricine SDS-PAGE is used and nitrocellulose filters are autoclaved after the transfer and before blocking and addition of anti-Ub antibodies. The C-terminus of ubiquitin forms an isopeptide bond with the e-amino group of a lysine side chain in a target protein. In this way proteins can be covalently modified by the addition of ubiquitin which may alter the target protein's function. Monoubiquitination generally targets proteins for internalization, endocytosis and lysosomal degradation, or modifies the surface charge of histones and affects chromatin compaction. Conjugation of ubiquitin (Ub) involves a three-step mechanism whereby specific enzymes (or enzyme complexes) activate and covalently link Ub to their substrates.
- 纯化方法
- purified
- 过滤
- Sterile filtered
- 免疫原
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This purified antibody was prepared from rabbit serum after repeated immunizations with ubiquitin coupled to rabbit IgG using glutaraldehyde.
Immunogen Type: NativeProtein - 亚型
- IgG
- Top Product
- Discover our top product Ubiquitin Primary Antibody
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anti-Ubiquitin (Ubiquitin) antibody
Ubiquitin 适用: Cow WB, IHC, IF, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-Ubiquitin (Ubiquitin) (AA 1-229) antibodyUbiquitin 适用: 人, 小鼠, 大鼠, Cow, Pig, 兔, 犬, 马 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-Ubiquitin (Ubiquitin) (AA 1-229) antibodyUbiquitin 适用: 人, 大鼠 WB, IHC, ICC, IP 宿主: 小鼠 Monoclonal D2 unconjugated
anti-Ubiquitin (Ubiquitin) (acLys27) antibodyUbiquitin 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Ubiquitin (Ubiquitin) antibody (HRP)Ubiquitin 适用: Cow WB, IHC, IF, ICC, IP 宿主: 兔 Polyclonal HRP
anti-Ubiquitin (Ubiquitin) antibody (Alkaline Phosphatase (AP))Ubiquitin 适用: Cow WB, IHC, IF, ICC, IP 宿主: 兔 Polyclonal Alkaline Phosphatase (AP)
anti-Ubiquitin (Ubiquitin) antibody (FITC)Ubiquitin 适用: Cow WB, IHC, IF, ICC, IP 宿主: 兔 Polyclonal FITC
anti-Ubiquitin (Ubiquitin) antibody (Biotin)Ubiquitin 适用: Cow WB, IHC, IF, ICC, IP 宿主: 兔 Polyclonal Biotin
anti-Ubiquitin (Ubiquitin) antibody (PE)Ubiquitin 适用: Cow WB, IHC, IF, ICC, IP 宿主: 兔 Polyclonal PE
anti-Ubiquitin (Ubiquitin) antibody (PerCP)Ubiquitin 适用: Cow WB, IHC, IF, ICC, IP 宿主: 兔 Polyclonal PerCP
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- 应用备注
- This purified polyclonal antibody reacts with ubiquitin by ELISA and western blot. Although not tested, this antibody is likely functional in immunohistochemistry and immunoprecipitation. For detection of free Ub by western blotting use Tris-Tricine SDS-PAGE and autoclaved nitrocellulose filters after the transfer and before blocking and addition of anti-Ub antibodies. Details on western blotting procedures are found in Mimnaugh et al., (1999 and 2002).
- 说明
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Gene Name: UBB
Post Translational Modification: Phosphorylation - 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1.0 mg/mL
- 缓冲液
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C/-20 °C
- 储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.
- 有效期
- 12 months
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: "The measurement of ubiquitin and ubiquitinated proteins." in: Electrophoresis, Vol. 20, Issue 2, pp. 418-28, (1999) (PubMed).
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: "The measurement of ubiquitin and ubiquitinated proteins." in: Electrophoresis, Vol. 20, Issue 2, pp. 418-28, (1999) (PubMed).
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- 抗原
- Ubiquitin
- 别名
- Ubiquitin (Ubiquitin 产品)
- 背景
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Ubiquitin (Ub) is a small, 76-residue, protein (8.5 kDa) found both as free monomer and covalently attached to itself and other proteins in eukaryotic cells. Free Ub is a very compact and stable molecule that is easily refolded after being denatured. It is therefore recommended that for detection of free Ub on Westerns, the Tris-Tricine SDS-PAGE is used and nitrocellulose filters are autoclaved after the transfer and before blocking and addition of anti-Ub antibodies. The C-terminus of ubiquitin forms an isopeptide bond with the e-amino group of a lysine side chain in a target protein. In this way proteins can be covalently modified by the addition of ubiquitin which may alter the target protein's function. Monoubiquitination generally targets proteins for internalization, endocytosis and lysosomal degradation, or modifies the surface charge of histones and affects chromatin compaction. Conjugation of ubiquitin (Ub) involves a three-step mechanism whereby specific enzymes (or enzyme complexes) activate and covalently link Ub to their substrates.
Synonyms: FLJ25987 antibody, MGC8385 antibody, Polyubiquitin B antibody, RPS27A antibody, UBA52 antibody, UBA80 antibody, UBB antibody, UBC antibody, UBCEP1 antibody - 途径
- Mitotic G1-G1/S Phases, Ubiquitin Proteasome Pathway
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